The omicization of agrochemical research

Wheelock, Craig E.; Miyagawa, Hisashi

doi:10.1584/jpestics.31.240

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…Intensive efforts have been undertaken in the last decades to discover new compounds with favorable environmental and safety features to selectively control weeds. Recently, this aim has been pursued with new strategies, switching from the classical testing of chemicals for efficacy on whole plants toward either the use of in vitro assays against a given molecular target (e.g., 1, 2) or a proteomic/metabolomic approach to rapidly identify the unknown mode of action of a given compound endowed with biological activity (e.g., 3,4). Among these substances, the most remarkable examples to date are provided by a naturally occurring glutamate analogue, phosphinothricin (PPT) (5), and a synthetic inhibitor of the shikimate pathway enzyme 5-enol-pyruvyl-shikimate-3-phosphate (EPSP) synthase (EC 2.5.…”

Section: Introductionmentioning

confidence: 99%

Plant P5C Reductase as a New Target for Aminomethylenebisphosphonates

Forlani

Giberti

Berlicki

et al. 2007

J. Agric. Food Chem.

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A series of N-substituted derivatives of aminomethylenebisphosphonic acid were evaluated as potential inhibitors of delta1-pyrroline-5-carboxylate reductase (EC 1.5.1.2), the enzyme that catalyzes the last step in proline biosynthesis, partially purified from Arabidopsis thaliana suspension cultured cells. At millimolar concentrations, three compounds out of 26 were found to interfere with the catalytic mechanism. One of them, namely, 3,5-dichloropyridyl-aminomethylenebisphosphonic acid, retained such inhibitory activity in the micromolar range. Kinetic analyses ruled out the possibility that the inhibition could simply rely upon the chelating properties of bisphosphonates and showed mechanisms of a noncompetitive type against NADH and an uncompetitive type against delta1-pyrroline-5-carboxylic acid, with KI values of 199 +/- 6 and 10.3 +/- 1.5 microM, respectively. A computer-aided docking analysis, performed on the basis of the crystal structure of the enzyme from Streptococcus pyogenes, suggested that this phosphonate may interact with amino acid residues near the binding site of delta1-pyrroline-5-carboxylic acid, thus blocking the substrate in a pocket and preventing its interaction with NADH. Because in higher plants the step catalyzed by delta1-pyrroline-5-carboxylate reductase is shared by all pathways leading to proline synthesis, such a compound may represent a lead structure to be exploited for the design of new substances endowed with herbicidal activity.

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Section: Introductionmentioning

confidence: 99%

Plant P5C Reductase as a New Target for Aminomethylenebisphosphonates

Forlani

Giberti

Berlicki

et al. 2007

J. Agric. Food Chem.

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“…The continued evolution of agrochemistry depends upon the adoption of novel methods to target discovery, mode of action and lead compound identification. The use of "omics" technologies is a logical approach to expanding the arsenal of tools available in this important industry [150].…”

Section: Integrated "Omics" Approaches In the Study Of Plant Responsementioning

confidence: 99%

Metabolomics - Useful Tool for Study of Plant Responses to Abiotic Stresses

Kráľová¹,

Jampílek²,

Ostrovský³

2012

Ecological Chemistry and Engineering S

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Abiotic stresses are produced by inappropriate levels of physical components of the environment and cause plant injury through unique mechanisms that result in specific responses. Metabolomics is a relatively new approach aimed at improved understanding of metabolic networks and the subsequent biochemical composition of plants and other biological organisms. The paper is focused on the use of metabolomics, metabolic profiling and metabolic fingerprinting to study plant responses to some environmental stresses (eg elevated temperature, chilling and freezing, drought, high salinity, UV radiation, high ozone levels, nutrient deficiency, oxidative stress, herbicides and heavy metals). Attention is also devoted to the effects of some environmental factors on plants such as high or low levels of CO2 or different levels of irradiance. Alterations of plants metabolites due to multiple abiotic stresses (drought-heat, drought-salinity, elevated CO2-salinity) are analysed as well. In addition, metabolomic approach to study plant responses to some artificial abiotic stresses, mechanical stress or pulsed electric field-induced stress is discussed. The most important analytical methods applied in metabolomics are presented and perspectives of metabolomics exploitation in the future are outlined, too.

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“…Another important technology is OMICS (Wheelock and Miyagawa 2006 ) , where genes, gene expression, proteins as well as their metabolites (metabolome) are comprehensively studied. OMICS information seems to be clinically helpful in tailormade remedies and the individualization of drug therapies.…”

Section: Informaticsmentioning

confidence: 99%