The odontogenic performance of human dental pulp stem cell in 3-dimensional chitosan and nano-bioactive glass-based scaffold material with different pores size

Morsy, Reham A.A.; Beherei, Hanan H.; Ellithy, Marwa M; Tarek, Heba E; Mabrouk, Mostafa

doi:10.4103/jasmr.jasmr_18_19

Cited by 7 publications

(3 citation statements)

References 3 publications

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“…These data indicate that higher pore size facilitates increased DPSCs migration and proliferation, in agreement with a previous report on culturing DPSCs into 3D poly-L-lactic acid-based scaffolds [51]. In addition, the mechanical properties of scaffolds with overly large pores are compromised, whereas higher cellular proliferation within large pore sizes can have implications for differentiation [52]. Our cytotoxicity findings are consistent with several other studies using GO layer cultured with mesenchymal stromal cells and GO/chitosan scaffolds seeded with human adipose-derived stem cells [53,54].…”

Section: Discussionsupporting

confidence: 91%

Biodegradable and Biocompatible Graphene-based Scaffolds for Functional Neural Tissue Engineering: A Strategy Approach Using Dental Pulp Stem Cells and Biomaterials

Mansouri

Al-Sarawi

Lošić

et al. 2021

Preprint

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Neural tissue engineering aims to restore function of nervous system tissues using biocompatible cell-seeded scaffolds. Graphene-based scaffolds combined with stem cells deserve special attention to enhance tissue regeneration in a controlled manner. However, it is believed that minor changes in scaffold biomaterial composition, internal porous structure, and physicochemical properties can impact cellular growth and adhesion. The current work aims to investigate in vitro biological effects of 3D graphene oxide (GO)/sodium alginate (GOSA) and reduced GOSA (RGOSA) scaffolds on dental pulp stem cells (DPSCs) in terms of cell viability and cytotoxicity. Herein, the effects of the 3D scaffolds, coating conditions, and serum supplementation on DPSCs functions are explored extensively. Biodegradation analysis revealed that addition of GO enhanced the degradation rate of composite scaffolds. Compared to the 2D surface, the cell viability of 3D scaffolds was higher (p <0.0001), highlighting the optimal initial cell adhesion to the scaffold surface and cell migration through pores. Moreover, the cytotoxicity study indicated that the incorporation of graphene supported higher DPSCs viability. It is also shown that when the mean pore size of scaffold increases, DPSCs activity decreases. In terms of coating conditions, poly-l-lysine (PLL) was the most robust coating reagent that improved cell-scaffold adherence and DPSCs metabolism activity. The cytotoxicity of GO-based scaffolds showed that DPSCs can be seeded in serum-free media without cytotoxic effects. This is critical for human translation as cellular transplants are typically serum-free. These findings suggest that proposed 3D GO-based scaffolds have favourable effects on the biological responses of DPSCs.

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Section: Discussionsupporting

confidence: 91%

Biodegradable and Biocompatible Graphene-based Scaffolds for Functional Neural Tissue Engineering: A Strategy Approach Using Dental Pulp Stem Cells and Biomaterials

Mansouri

Al-Sarawi

Lošić

et al. 2021

Preprint

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show abstract

“…These data indicate that higher pore size facilitates increased DPSCs migration and proliferation, in agreement with a previous report on culturing DPSCs into 3D PLL acid‐based scaffolds (Conde et al, 2015). In addition, the mechanical properties of scaffolds with overly large pores are compromised, whereas higher cellular proliferation within large pore sizes can have implications for differentiation (Morsy et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Biodegradable and biocompatible graphene‐based scaffolds for functional neural tissue engineering: A strategy approach using dental pulp stem cells and biomaterials

Mansouri

Al-Sarawi

Lošić

et al. 2021

Biotech & Bioengineering

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Neural tissue engineering aims to restore the function of nervous system tissues using biocompatible cell-seeded scaffolds. Graphene-based scaffolds combined with stem cells deserve special attention to enhance tissue regeneration in a controlled manner. However, it is believed that minor changes in scaffold biomaterial composition, internal porous structure, and physicochemical properties can impact cellular growth and adhesion. The current work aims to investigate in vitro biological effects of three-dimensional (3D) graphene oxide (GO)/sodium alginate (GOSA) and reduced GOSA (RGOSA) scaffolds on dental pulp stem cells (DPSCs) in terms of cell viability and cytotoxicity. Herein, the effects of the 3D scaffolds, coating conditions, and serum supplementation on DPSCs functions are explored extensively. Biodegradation analysis revealed that the addition of GO enhanced the degradation rate of composite scaffolds. Compared to the 2D surface, the cell viability of 3D scaffolds was higher (p < 0.0001), highlighting the optimal initial cell adhesion to the scaffold surface and cell migration through pores. Moreover, the cytotoxicity study indicated that the incorporation of graphene supported higher DPSCs viability. It is also shown that when the mean pore size of the scaffold increases, DPSCs activity decreases. In terms of coating conditions, poly-L-lysine was the most robust coating reagent that improved cell-scaffold adherence and DPSCs metabolism activity. The cytotoxicity of GO-based scaffolds showed that DPSCs can be seeded in serum-free media without cytotoxic effects. This is critical for human translation as cellular transplants

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“…Due to the low strength of chitosan, bioactive glasses (BGs) have been widely added to pure chitosan to improve the mechanical properties. According to the literature [26,27,29,44] increasing of bioactive glass, increased mechanical properties. Pure chitosan scaffold showed 0.11 MPa compressive strength, while enhancing bioactive glass to pure chitosan increased compressive strength (2-12 MPa)[7].…”

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confidence: 99%

Composition optimization of Bioactive glass /Chitosan /Zeolite ternary bio- composite

Moghaddam

Oroujzadeh

Salehirad

2022

Preprint

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To achieve the desired mechanical and biological properties, the weight percentage of each component in bioactive glass/chitosan/zeolite bio-composite fabricated via the liquid phase method was optimized. In the first step: the weight percentage of zeolite was kept constant and the amounts of the other two components were modified considering the mechanical properties. In the second step, to optimize the weight percentage of the zeolite, the best sample of the first step was re-fabricated with different zeolite percentages (5 to 20 wt.%). All the bio-composites were identified by FT-IR, XRD, FE-SEM, and EDS, then their mechanical properties were measured. To determine the biological activity, samples were immersed in SBF for 1, 3, and 7 days and the hydroxyapatite growth rate was measured by XRD. Results specified that the sample with 65 wt.% bioactive glass/20 wt.% chitosan/15 wt.% zeolite had the best mechanical properties and had excellent hydroxyapatite growth in three days.

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The odontogenic performance of human dental pulp stem cell in 3-dimensional chitosan and nano-bioactive glass-based scaffold material with different pores size

Cited by 7 publications

References 3 publications

Biodegradable and Biocompatible Graphene-based Scaffolds for Functional Neural Tissue Engineering: A Strategy Approach Using Dental Pulp Stem Cells and Biomaterials

Biodegradable and Biocompatible Graphene-based Scaffolds for Functional Neural Tissue Engineering: A Strategy Approach Using Dental Pulp Stem Cells and Biomaterials

Biodegradable and biocompatible graphene‐based scaffolds for functional neural tissue engineering: A strategy approach using dental pulp stem cells and biomaterials

Composition optimization of Bioactive glass /Chitosan /Zeolite ternary bio- composite

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