The octadecanoid signaling pathway participates in the chilling-induced transcription of ω-3 fatty acid desaturases in Arabidopsis

Shi, Yulan; An, Lizhe; Li, Xinrong; Huang, Caihong; Chen, Guoxiong

doi:10.1016/j.plaphy.2010.11.013

Cited by 16 publications

(23 citation statements)

References 48 publications

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“…Together, these studies indicate that FAD7 has contrasting effects on different stress responses, helping defend against some stresses but increasing susceptibility to others. Consistent with this, levels of FAD7 gene expression are responsive to changing conditions, including temperature, wounding, and other stresses (Nishiuchi et al, 1997;Yaeno et al, 2006;Shi et al, 2011).…”

Section: Introductionsupporting

confidence: 59%

Loss of Function of Fatty Acid Desaturase 7 in Tomato Enhances Photosynthetic Carbon Fixation Efficiency

et al. 2020

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Fatty Acid Desaturase 7 (FAD7) generates polyunsaturated fatty acids, promoting the desaturation of chloroplast membranes; it also provides an essential precursor for the synthesis of jasmonic acid (JA), a phytohormone that can influence plant growth, development, and primary metabolism. This study examined the effects of spr2, a null mutation in SlFAD7, on the growth, morphology, and photosynthetic traits of tomato, Solanum lycopersicum. Although the spr2 mutant had a lower density of stomata than wild type plants, the two genotypes had comparable stomatal conductance, transpiration rates, and intracellular CO 2 levels; in addition, spr2 had significantly thinner leaf blades, which may help maintain normal levels of CO 2 diffusion despite the lower number of stomata. Surprisingly, spr2 also had significantly higher carbon assimilation (A) and maximum quantum efficiency of PSII (F v /F m) than wild type plants at both of the light intensities tested here (220 or 440 µmol m −2 s −1), despite having lower levels of chlorophyll than wild type plants under low light (220 µmol m −2 s −1). Furthermore, CO 2 response curves indicated higher in vivo Rubisco activity (V cmax) in spr2 compared to wild type plants, as well as an enhanced maximum rate of electron transport used in the regeneration of ribulose-1,5-bisphosphate (J max). These data indicate that loss of function of FAD7 can enhance the efficiency of both light-dependent and light-independent reactions in photosynthesis. Consistent with this, the spr2 mutant also displayed enhanced growth, with significantly more leaves and a more compact growth habit. In contrast to spr2, another tomato mutant impaired in JA synthesis (acx1) showed no enhancements in growth or photosynthetic efficiency, suggesting that the enhancements observed in spr2 are independent of the effects of this mutation on JA synthesis. These data demonstrate that loss of function of FAD7 can enhance photosynthesis and growth, potentially through its impacts on the chloroplast membranes.

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Section: Introductionsupporting

confidence: 59%

Loss of Function of Fatty Acid Desaturase 7 in Tomato Enhances Photosynthetic Carbon Fixation Efficiency

et al. 2020

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“…S3). Moreover, Arabidopsis cold acclimation results in the induction of crucial genes in the biosynthesis of unsaturated fatty acids such as FAD3, FAD7 and FAD8 (Shi et al 2011), and an enhancement of membrane lipid desaturation (Uemura et al 1995). We failed, however, to find an upregulation of these genes in tomato plants exposed to 10°C (Supporting Information Fig.…”

Section: Discussionmentioning

confidence: 84%

“…Moreover, Arabidopsis cold acclimation results in the induction of crucial genes in the biosynthesis of unsaturated fatty acids such as FAD3 , FAD7 and FAD8 (Shi et al . ), and an enhancement of membrane lipid desaturation (Uemura et al . ).…”

Section: Discussionmentioning

confidence: 99%

Tomato plants increase their tolerance to low temperature in a chilling acclimation process entailing comprehensive transcriptional and metabolic adjustments

Barrero-Gil

Huertas

Rambla

et al. 2016

Plant Cell & Environment

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Low temperature is a major environmental stress that seriously compromises plant development, distribution and productivity. Most crops are from tropical origin and, consequently, chilling sensitive. Interestingly, however, some tropical plants, are able to augment their chilling tolerance when previously exposed to suboptimal growth temperatures. Yet, the molecular and physiological mechanisms underlying this adaptive process, termed chilling acclimation, still remain practically unknown. Here, we demonstrate that tomato plants can develop a chilling acclimation response, which includes comprehensive transcriptomic and metabolic adjustments leading to increased chilling tolerance. More important, our results reveal strong resemblances between this response and cold acclimation, the process whereby plants from temperate regions raise their freezing tolerance after exposure to low, non-freezing temperatures. Both chilling and cold acclimation are regulated by a similar set of transcription factors and hormones, and share common defence mechanisms, including the accumulation of compatible solutes, the mobilization of antioxidant systems and the rearrangement of the photosynthetic machinery. Nonetheless, we have found some important divergences that may account for the freezing sensitivity of tomato plants. The data reported in this manuscript should foster new research into the chilling acclimation response with the aim of improving tomato tolerance to low temperature.

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“…Motif #2 (Fig. 5 b) contains two genes involved in the response to cold, MDP0000205588, encoding an osmotic fatty acid desaturase [ 56 ] and MDP0000249561, the BZIP44 transcription factor also putatively involved in the control of fruit maturation through cell wall loosening [ 57 , 58 ]; and two genes involved in response to oxidative stress: MDP0000309881, similar to the hypoxia-induced gene domain 1 [ 59 ], and MDP0000920069, encoding a respiratory burst oxidase protein F [ 60 ] also responsive to ethylene and abscisic acid. Within this motif, only the gene MDP0000252890 encoding a rubisco subunit 3B, could not be directly related to the studied process according to scientific literature.…”

Section: Resultsmentioning

confidence: 99%

Large scale study of anti-sense regulation by differential network analysis

et al. 2018

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BackgroundSystems biology aims to analyse regulation mechanisms into the cell. By mapping interactions observed in different situations, differential network analysis has shown its power to reveal specific cellular responses or specific dysfunctional regulations. In this work, we propose to explore on a large scale the role of natural anti-sense transcription on gene regulation mechanisms, and we focus our study on apple (Malus domestica) in the context of fruit ripening in cold storage.ResultsWe present a differential functional analysis of the sense and anti-sense transcriptomic data that reveals functional terms linked to the ripening process. To develop our differential network analysis, we introduce our inference method of an Extended Core Network; this method is inspired by C3NET, but extends the notion of significant interactions. By comparing two extended core networks, one inferred with sense data and the other one inferred with sense and anti-sense data, our differential analysis is first performed on a local view and reveals AS-impacted genes, genes that have important interactions impacted by anti-sense transcription. The motifs surrounding AS-impacted genes gather transcripts with functions mostly consistent with the biological context of the data used and the method allows us to identify new actors involved in ripening and cold acclimation pathways and to decipher their interactions. Then from a more global view, we compute minimal sub-networks that connect the AS-impacted genes using Steiner trees. Those Steiner trees allow us to study the rewiring of the AS-impacted genes in the network with anti-sense actors.ConclusionAnti-sense transcription is usually ignored in transcriptomic studies. The large-scale differential analysis of apple data that we propose reveals that anti-sense regulation may have an important impact in several cellular stress response mechanisms. Our data mining process enables to highlight specific interactions that deserve further experimental investigations.Electronic supplementary materialThe online version of this article (10.1186/s12918-018-0613-7) contains supplementary material, which is available to authorized users.

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The octadecanoid signaling pathway participates in the chilling-induced transcription of ω-3 fatty acid desaturases in Arabidopsis

Cited by 16 publications

References 48 publications

Loss of Function of Fatty Acid Desaturase 7 in Tomato Enhances Photosynthetic Carbon Fixation Efficiency

Loss of Function of Fatty Acid Desaturase 7 in Tomato Enhances Photosynthetic Carbon Fixation Efficiency

Tomato plants increase their tolerance to low temperature in a chilling acclimation process entailing comprehensive transcriptional and metabolic adjustments

Large scale study of anti-sense regulation by differential network analysis

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