The occurrence of treponemes in contagious ovine digital dermatitis and the characterisation of associated

Moore, Lindsey; Woodward, MJ; Grogono-Thomas, Rosemary

doi:10.1016/j.vetmic.2005.10.016

Cited by 67 publications

(68 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To ensure validity in each assay, water was used as a negative control, and positive controls included genomic DNA from each of the three treponeme groups. All biopsy extractions were further subjected to a Treponema genusspecific PCR assay as originally described (21). All PCR primers used are listed in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

et al. 2009

View full text Add to dashboard Cite

This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes.

show abstract

Section: Methodsmentioning

confidence: 99%

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

et al. 2009

View full text Add to dashboard Cite

show abstract

“…putidum-like"), as described previously (32,34), with the resulting PCR products encompassing 300 to 500 bp of the 16S rRNA gene. All foot samples were also subjected to the Treponema genus PCR assay which detects all Treponema species, both pathogenic and commensal (37). To ensure validity in each assay, water was used as a negative control, and positive controls included genomic DNA from each of the three treponeme phylogroups.…”

Section: Genus-and Phylogroup-specific Treponeme Pcr Assaysmentioning

confidence: 99%

“…There is a clear association with certain phylogroups of treponemes for BDD, and these treponemes are present only in BDD lesions and are completely absent from healthy bovine tissues and most environmental and fecal samples (32,39). Therefore, the previous CODD study (37) provides no specific data on the presence/association of BDD-associated Treponema species with CODD lesions. Similarly, the remaining bacteriological data on CODD lesions comprised of a study based entirely on culturedependent methods, using only 10 samples; only 7 treponemal cultures were produced from the 10 samples, and only 2 pure treponeme isolates were obtained (20).…”

mentioning

confidence: 98%

“…Previous studies have investigated the association of Treponema bacteria with CODD lesions (20,37) and hypothesized that CODD was derived from BDD lesions in dairy cows (38). To date, little substantiating molecular evidence has been produced, and the possible involvement of other organisms such as Dichelobacter nodosus and Fusobacterium necrophorum has been proposed (37).…”

mentioning

confidence: 99%

“…To date, little substantiating molecular evidence has been produced, and the possible involvement of other organisms such as Dichelobacter nodosus and Fusobacterium necrophorum has been proposed (37). In a previous study, D. nodosus, F. necrophorum, and also treponemal bacteria were detected in a considerable proportion of CODD lesions (74%, 86%, and 70%, respectively) (37), but these bacteria were also detected in a substantial proportion of healthy foot tissue (31%, 46%, and 38%, respectively).…”

mentioning

confidence: 99%

See 2 more Smart Citations

High-Level Association of Bovine Digital Dermatitis Treponema spp. with Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium necrophorum and Dichelobacter nodosus

Sullivan¹,

Clegg²,

Angell

et al. 2015

J Clin Microbiol

View full text Add to dashboard Cite

Digital dermatitis (DD) is an infectious lameness commonly found in dairy cattle worldwide, and it is known as bovine digital dermatitis (BDD) or papillomatous digital dermatitis (PDD). The disease was first reported in 1980 in the United States (1) and in the late 1980s in the United Kingdom (2). BDD has also been confirmed in beef cattle (3, 4), and over the last 30 years, the disease has been recognized as an important cause of bovine lameness (5).Lameness in cattle and sheep has serious animal welfare and economic implications (6-9). The effects of lameness in cattle include a decrease in milk yield (9, 10) and fertility (8, 11-13) and an increase in rate of culling (12,14). This has been found to be particularly true for cattle suffering from BDD (8, 15, 16) with a recent study of the cost of lameness in the United States estimating that on average, BDD costs $133 per case (17).BDD is now a worldwide problem, and controlling BDD on dairy operations has proven difficult. Moreover, in the last 20 years, sheep in the United Kingdom have been identified with a form of DD termed contagious ovine digital dermatitis (CODD), which is rapidly emerging as a severe infectious foot disease since it was first reported in the United Kingdom in 1997 (18-20). Now, CODD has spread into the Republic of Ireland (20), and it was recently reported in dairy goats in the United Kingdom (21), indicating further cross-species transmission. The contagious nature of DD is also evident by the reports of a manifestation of the disease in a wildlife host, North American elk (Cervus elaphus) from Washington State (22). The reports of DD in previously unaffected species, including U.S. wildlife, suggests a much greater global threat of the disease than previously considered.BDD in cattle manifests in several forms, but most frequently as an ulcerative lesion of the digital skin located immediately above the coronary band between the heel bulbs which results in severe lameness (23). The clinical features of CODD in sheep are slightly different, mainly because the initial lesion site on the sheep foot is different. CODD lesions commence at the coronary band and then run under the hoof horn capsule dorsally and abaxially (24). CODD frequently presents a particularly severe outcome where the whole horn capsule can be lost (18,(25)(26)(27). As a result of the severity of the lesions, sheep can be extremely lame, impacting the welfare of the affected sheep (28). This is concurrent with the lesion pathology identified in elk, described as erosive lesions on

show abstract

Impact of research on contagious ovine digital dermatitis on the knowledge and practices of UK sheep farmers and veterinarians

Duncan

Angell²,

Grove‐White

et al. 2021

Veterinary Record

View full text Add to dashboard Cite

Background: Contagious ovine digital dermatitis (CODD) is a common foot disease of sheep which causes a severe form of lameness and can be difficult to control. Recent research has provided evidence-based guidance on diagnosis, treatment and farm management control. The aim of this study was to determine the uptake of this guidance on the knowledge and practices of UK sheep farmers and veterinarians and identify priorities for future research. Methods: Data were collected in 2019-2020 by electronic surveys of UK sheep veterinarians and farmers distributed through sheep industry organisations and social media. Results: Two hundred eight-four sheep farmers and 77 veterinarians responded to the surveys. Fifty-two per cent of farmers and 70% of vets considered that their management of CODD had improved as a direct result of recent research evidence on the disease. The principle areas improved for both sectors were biosecurity advice and use of antibiotic treatments. Farmers and veterinarians reported that the priorities for future research should be in therapeutics, vaccine development and the understanding of disease transmission. Conclusion: There has been strong uptake of recent evidence-based veterinary medicine by farmers and veterinarians for the management of CODD, particularly in the areas of biosecurity and responsible antibiotic use.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

The occurrence of treponemes in contagious ovine digital dermatitis and the characterisation of associated

Cited by 67 publications

References 14 publications

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

High-Level Association of Bovine Digital Dermatitis Treponema spp. with Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium necrophorum and Dichelobacter nodosus

Impact of research on contagious ovine digital dermatitis on the knowledge and practices of UK sheep farmers and veterinarians

Contact Info

Product

Resources

About