The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China)

Yang, Dong; Qiu, Zhigang; Shen, Zhiqiang; Zhao, Hong; Jin, Min; Li, Huaying; Liu, Weili; Lǐ, Jùnwén

doi:10.3390/ijerph14060576

Cited by 47 publications

(31 citation statements)

References 33 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A systematic screening of the gene in colistin-resistant strains was performed [150] (Figure 2). For such purposes, scientists have used the primers of the original study [6], or have designed their own primers for standard PCR [132, 151–160] or RT-PCR, based on SYBR Green assays [64, 151, 161], TaqMan probe [66, 72, 152, 162], or other FAM-labelled probe [9, 71, 163, 164] or HEX-labelled probe [165] (Table 4).…”

Section: Genotyping and Molecular Screeningmentioning

confidence: 99%

Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains

Bardet

Rolain

2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

The recent discovery of the plasmid-mediated mcr-1 gene conferring resistance to colistin is of clinical concern. The worldwide screening of this resistance mechanism among samples of different origins has highlighted the urgent need to improve the detection of colistin-resistant isolates in clinical microbiology laboratories. Currently, phenotypic methods used to detect colistin resistance are not necessarily suitable as the main characteristic of the mcr genes is the low level of resistance that they confer, close to the clinical breakpoint recommended jointly by the CLSI and EUCAST expert systems (S ≤ 2 mg/L and R > 2 mg/L). In this context, susceptibility testing recommendations for polymyxins have evolved and are becoming difficult to implement in routine laboratory work. The large number of mechanisms and genes involved in colistin resistance limits the access to rapid detection by molecular biology. It is therefore necessary to implement well-defined protocols using specific tools to detect all colistin-resistant bacteria. This review aims to summarize the current clinical microbiology diagnosis techniques and their ability to detect all colistin resistance mechanisms and describe new tools specifically developed to assess plasmid-mediated colistin resistance. Phenotyping, susceptibility testing, and genotyping methods are presented, including an update on recent studies related to the development of specific techniques.

show abstract

Section: Genotyping and Molecular Screeningmentioning

confidence: 99%

Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains

Bardet

Rolain

2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

show abstract

“…have also been documented. Furthermore, mcr-1 has been observed in bacteria from wild animals and water samples, indicating the resistance determinant has also disseminated into the environment 194 , 224 , 226 – 230 .…”

Section: Colistinmentioning

confidence: 99%

Illustrative examples of probable transfer of resistance determinants from food animals to humans: Streptothricins, glycopeptides, and colistin

Webb

Angulo

Granier³

et al. 2017

F1000Res

View full text Add to dashboard Cite

Use, overuse, and misuse of antimicrobials contributes to selection and dissemination of bacterial resistance determinants that may be transferred to humans and constitute a global public health concern. Because of the continued emergence and expansion of antimicrobial resistance, combined with the lack of novel antimicrobial agents, efforts are underway to preserve the efficacy of current available life-saving antimicrobials in humans. As a result, uses of medically important antimicrobials in food animal production have generated debate and led to calls to reduce both antimicrobial use and the need for use. This manuscript, commissioned by the World Health Organization (WHO) to help inform the development of the WHO guidelines on the use of medically important antimicrobials in food animals, includes three illustrations of antimicrobial use in food animal production that has contributed to the selection—and subsequent transfer—of resistance determinants from food animals to humans. Herein, antimicrobial use and the epidemiology of bacterial resistance are described for streptothricins, glycopeptides, and colistin. Taken together, these historical and current narratives reinforce the need for actions that will preserve the efficacy of antimicrobials.

show abstract

“…To date, mcr-1 has been observed on a wide variety of plasmid types, including IncI2, IncHI2, and IncX4 17 . Intensive screening efforts for mcr-1 have found it to be highly prevalent in a number of environmental settings, including the Haihe River in China 18 , recreational water at public urban beaches in Brazil 19 , and fecal samples from otherwise healthy individuals 20 , 21 . Although both Brazil and China have now banned the use of colistin in agriculture, the evidence that mcr-1 can spread within hospital environments even in the absence of colistin use 22 as well as in the community 21 raises the possibility that the spread of mcr-1 will not be contained by these bans.…”

Section: Introductionmentioning

confidence: 99%

The global distribution and spread of the mobilized colistin resistance gene mcr-1

et al. 2018

View full text Add to dashboard Cite

Colistin represents one of the few available drugs for treating infections caused by carbapenem-resistant Enterobacteriaceae. As such, the recent plasmid-mediated spread of the colistin resistance gene mcr-1 poses a significant public health threat, requiring global monitoring and surveillance. Here, we characterize the global distribution of mcr-1 using a data set of 457 mcr-1-positive sequenced isolates. We find mcr-1 in various plasmid types but identify an immediate background common to all mcr-1 sequences. Our analyses establish that all mcr-1 elements in circulation descend from the same initial mobilization of mcr-1 by an ISApl1 transposon in the mid 2000s (2002–2008; 95% highest posterior density), followed by a marked demographic expansion, which led to its current global distribution. Our results provide the first systematic phylogenetic analysis of the origin and spread of mcr-1, and emphasize the importance of understanding the movement of antibiotic resistance genes across multiple levels of genomic organization.

show abstract

The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China)

Cited by 47 publications

References 33 publications

Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains

Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains

Illustrative examples of probable transfer of resistance determinants from food animals to humans: Streptothricins, glycopeptides, and colistin

The global distribution and spread of the mobilized colistin resistance gene mcr-1

Contact Info

Product

Resources

About