The occurrence of polyadenylate sequences in bacteria and yeast

Edmonds, Mary; Kopp, David W.

doi:10.1016/0006-291x(70)90561-9

Cited by 21 publications

(4 citation statements)

References 9 publications

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“…The occurrence of polyriboadenylic acid and of an enzyme capable of synthesizing this polymer in eukaryotic cells has been known for years (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). In general, poly(A) isolated from several animal cells had a base composition of greater than 85% adenine and was about 200 nucleotides in length.…”

mentioning

confidence: 99%

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Sheldon

Jurale

Kates

1972

Proc. Natl. Acad. Sci. U.S.A.

208

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A rapid and specific technique to detect polyriboadenylic acid sequences in RNA is described. The method depends upon the ability of RNAs that contain poly(A) sequences to associate specifically with poly(U) that has been immobilized on fiberglass filters by ultraviolet irradiation. A high proportion of the transcripts synthesized in vivo and in vitro from the vaccinia virus genome contain poly(A) sequences and bind to the poly(U) filters. Similarly, DNA-like RNA from the nucleus and from the cytoplasmic polyribosomes of HeLa cells is rich in species that bind to poly(U) filters. Poly(U) immobilized on cellulose powder is useful to make columns with a high capacity for the binding and purification of poly(A)-containing RNAs.

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confidence: 99%

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Sheldon

Jurale

Kates

1972

Proc. Natl. Acad. Sci. U.S.A.

208

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“…In spite of a report claiming the absence of poly(A)+ RNA in E. coli (30), an increasing number of recent reports of its presence support the pioneering findings first reported from Edmonds' laboratory (9). For example, not only are these species of RNAs present, but they are heterodisperse, migrating from 20S to 4S regions when analyzed by denaturing formamide gel electrophoresis (25), and they can be varied from 15 to 1.5% as a function of the phosphate content of the growth media (38).…”

Section: Discussionmentioning

confidence: 85%

“…The importahce of these residues is also reflected by the fact that they play a significant role in the preparation of DNA complemenitary to the mRNA with reverse transcriptase (5,31,42). In the past few years, a number of studies have shown that a population of RNAs in the non-photosynthetic procaryotes also contains these residues at the 3'-OH ends (9,25,27,33,38), and that such RNAs exhibit messenger properties when assayed under optimal conditions (11,17).…”

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confidence: 99%

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Majumdar¹,

McFadden²

1984

J Bacteriol

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Total cellular RNA extracted fromh Rhodospirillum rubrum cultured in butyrate-containing medium under strict photosynthetic conditions to the stationary phase of growth has been fractionated on an oligodeoxythymidylic acid-cellulose column into polyadenylated [poly(A)'] RNA and poly(A)-RNA fractions. The poly(A)+ fraction was 9 to 10% of the total bulk RNA isolated. Atnalysis of the poly(A)+ RNA on a denaturing urea-polyacrylamide gel revealed four sharp bands of RNA distributed in heterodisperse fashion between 16S and 9S. Similar fractionation of the poly(A)-RNA resulted in the separation of 23, 16, and 5S rRNAs and 4S tRNA. Poly(A)+ fragments isolated after combined digestion with pancreatic A and T1 RNases and analysis by denaturing gel electrophoresis demonstrated two major components of 80 and 100 residues. Alkaline hydrolysis of the nuclease-resistant, purified residues showed AMP-rich nucleotides. Through the use of snake venom phosphodiesterase, poly(A) tracts were placed at the 3' end of poly(A)+ RNA. Stimtlation of [3H]leucine incorporation into hot trichloroacetic acid-precipitable polypeptides in a cell-free system from wheat germ primed by the poly(A)+ RNA mixture was found to be 220-fold higher than that for poly(A)-RNAs (on a unit mass basis), a finding which demonstrated that poly(A)+ RNAs in R. rubrum are mRNAs. Gel electrophoretic analysis of the translation mixture revealed numerous 3H-labeled products including a major band (Mr, 52,000). The parent protein was precipitated by antibodies to ribulose bisphosphate carboxylase-oxygenase and comprised 6.5% of the total translation products.

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“…10 for other references). This process of mRNA maturation involving poly(A) addition appears to occur generally in eukaryotes but not in bacteria (11,12).…”

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confidence: 99%

Poly(A) Polymerase and Poly(G) Polymerase in Wheat Chloroplasts

Burkard

Keller

1974

Proc. Natl. Acad. Sci. U.S.A.

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Extracts of wheat chloroplasts contain a poly(A) polymerase which can polymerize AMP residues from ATP onto an RNA primer. Whole extracts of wheat leaves also contain another poly(A) polymerase which is present in much larger amount and is probably derived from the nuclei. Both polymerases can utilize as primer poly(A), poly(C), transfer RNA, and ribosomal RNA, but only the chloroplast polymerase can utilize poly(U) and poly(G). Both enzymes have a specific requirement for ATP. Extracts of wheat chloroplasts contain, in addition to the poly(A) polymerase, a poly(G) polymerase which can polymerize GMP residues from GTP onto primers such as poly(G), poly(A), or ribosomal RNA. The poly(G) polymerase cannot utilize ATP but can slowly polymerize CMP from CTP. When the two chloroplast polymerases are present together in an in vitro incubation with ATP plus GTP and poly(A), the polymerization product is a mixed poly(A,G) tract.

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The occurrence of polyadenylate sequences in bacteria and yeast

Cited by 21 publications

References 9 publications

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA

Polyadenylated mRNA from the photosynthetic procaryote Rhodospirillum rubrum

Poly(A) Polymerase and Poly(G) Polymerase in Wheat Chloroplasts

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