The occurrence of callose during the process of local lesion formation

Shimomura, Toshiyasu; Dijkstra, Jeanne

doi:10.1007/bf01999861

Cited by 58 publications

(24 citation statements)

References 17 publications

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“…The localized deposition and speed of callose formation may be important in the first line of defense against pathogens as callose is also a major component of papillae or wall appositions which are formed at sites of attempted penetration by invading fungal hyphae (1,4). Callose is similarly found around lesions in certain virus-infected plants where it may help to prevent spreading of the virus (4,20).…”

mentioning

confidence: 99%

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca²⁺-Dependent Process

Köhle¹,

Jeblick²,

Poten³

et al. 1985

Plant Physiol.

342

143

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A new method for the rapid and quantitative fluorometric determination of callose is described. In suspension-cultured cells of Glycine max, synthesis of callose starts within 20 minutes of treatment with chitosan and parallels over hours the accumulation of 1,3-linked glucose in the wall. Poly-L-lysine also elicits callose synthesis. The effect of chitosan is enhanced by Polymyxin B at low concentrations; this antibiotic alone at higher concentrations can also induce callose synthesis. Callose synthesis is immediately stopped when external Ca2l is bound by ethylene glycolbis-(2-aminoethyl ether)-N,N'-tetraacetate or cation exchange beads, and partly recovers upon restoration of 15 micromolar Ca21.Callose synthesis is observed only when membrane perturbation causing electrolyte leakage from the cells is induced by one of the above treatments. It does not appear to be due to de novo synthesis or proteolytic activation of 1,3-#-D-glucan synthase. It is concluded that this Ca24-dependent enzyme is directly activated by the influx of Ca24 occurring concomitantly with the leakage of cell constituents. This suggestion is also discussed in conjunction with the chitosan-induced synthesis of phytoalexin in the same cells.

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mentioning

confidence: 99%

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca²⁺-Dependent Process

Köhle¹,

Jeblick²,

Poten³

et al. 1985

Plant Physiol.

342

143

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show abstract

“…Callose is produced after every mitotic division, and it is accumulated in small amounts in primary walls during somatic-type cytokinesis of meristem cells in various plants (Waterkeyn 1971;Longly and Waterkeyn 1977). Fluorescence characteristic of callose is detected in plant cells also in response to various stressors, such as pathogenous causing tissue damage (Shimoura and Dijkstra 1975). In sporocytes of flowering plants, callose is frequently observed in microsporocyte and megasporocyte walls where clear differences are noted in callose distribution patterns.…”

Section: Discussionmentioning

confidence: 99%

Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv.

Domaciuk¹,

Leszczuk²,

Szczuka³

et al. 2016

Polish Polar Research

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Abstract:The development of megasporocytes and the functional megaspore formation in Deschampsia antarctica were analyzed with the use of microscopic methods. A single archesporial cell was formed directly under the epidermis in the micropylar region of the ovule without producing a parietal cell. In successive stages of development, the meiocyte was transformed into a megaspore tetrad after meiosis. Most megaspores were arranged in a linear fashion, but some tetrads were T-shaped. Only one of the 60 analyzed ovules contained a cell in the direct proximity of the megasporocyte, which could be an aposporous initial. Most of the evaluated D. antarctica ovules featured monosporic embryo sacs of the Polygonum type. Approximately 30% of ovules contained numerous megaspores that were enlarged. The megaspores were located at chalazal and micropylar poles, and some ovules featured two megaspores -terminal and medial -in the chalazal region, or even three megaspores at the chalazal pole. In those cases, the micropylar megaspore was significantly smaller than the remaining megaspores, and it did not have the characteristic features of functional megaspores. Meiocytes and megaspores of D. antarctica contained polysaccharides that were detectable by PAS-reaction and aniline blue staining. Starch granules and cell walls of megasporocytes, megaspores and nucellar cells were PAS-positive. Fluorescent callose deposits were identified in the micropylar end of the megasporocytes. During meiosis and after its completion, thick callose deposits were also visible in the periclinal walls and in a small amount in the anticlinal walls of megaspores forming linear and T-shaped tetrads. Callose deposits fluorescence was not observed in the walls of the nucellar cells.

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“…Las proteínas R actúan como receptores y las proteínas inductoras como ligando (Keen, 1990;Gabriel y Rolfe 1990;Ellis et al, 2000b) y su interacción inicia una cascada de señales que desencadenan la respuesta hipersensible (HR), caracterizada por una muerte celular programada que induce lesiones necróticas en el sitio de la infección (Staskawicz et al, 1995;Heath, 2000;Shirasu y Schulze-Lefert, 2003, Soosaar et al, 2005. La HR va acompañada por una respuesta sistémica adquirida (Soosaar et al, 2005) en tejidos lejanos de la zona de infección, caracterizada por i) la producción de especies químicas oxidantes (Lamb y Dixon, 1997), ii) síntesis de callosa (Shimomura y Dijkstra, 1975) y lignina, iii) aumento de los niveles de ácido salicílico (Malamy et al, 1990;Naylor et al, 1998) y iv) producción de proteínas relacionadas con la patogénesis (Yalpani et al, 1991). De este modo la planta limita el movimiento a corta y larga distancia del patógeno.…”

Section: Genes De Resistencia (R)unclassified

Estudio de la interacción entre naranjo amargo y el virus de la tristeza de los cítricos

Serra¹

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The occurrence of callose during the process of local lesion formation

Cited by 58 publications

References 17 publications

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca²⁺-Dependent Process

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca²⁺-Dependent Process

Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv.

Estudio de la interacción entre naranjo amargo y el virus de la tristeza de los cítricos

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The occurrence of callose during the process of local lesion formation

Cited by 58 publications

References 17 publications

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca2+-Dependent Process

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca2+-Dependent Process

Female sporogenesis in the native Antarctic grass Deschampsia antarctica Desv.

Estudio de la interacción entre naranjo amargo y el virus de la tristeza de los cítricos

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Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca²⁺-Dependent Process

Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca²⁺-Dependent Process