One hundred and fifty-three strains of Escherichia coli were isolated from sporadic cases of gastroenteritis from cows, calves, buffaloes, buffalocalves, goats, and kids. Thirty-six biochemical tests were conducted. On the basis of fermentation reaction of six selected sugars (rhamnose, sucrose, dulcitol, raffinose, salicin, and starch), 153 strains were classified into 28 biotypes. Biotypes I, V, VI, XI, XVIII, and XX appeared more common than the others. These six biotypes accounted for over 60% of the total (153) strains isolated from diarrhoea, dysentery, or white scours. Biotype XX predominated in buffaloes and buffalocalves, and Type VI in cows and calves, and in goats and kids. Again Type VI predominated in diarrhoea and dysentery and Type XI in white scour. Only 137 strains could be identified serologically.