The occurrence of antimicrobial residues and antimicrobial resistance genes in urban drinking water and sewage in Southern Brazil

Ramalho, Rafaela; Mezzomo, Lisiane Cervieri; Machado, William C. Polônio; Hein, Camila da Silva Morais; Müller, Camila Zanfelice; Silva, Thaisla Cristiane Borella da; Jank, Louíse; Lamas, Alex Elias; Ballestrin, Rogério Antônio da Costa; Wink, Priscila Lamb; Lima, Anderson Araújo de; Corção, Gertrudes; Martins, Andreza Francisco

doi:10.1007/s42770-022-00786-2

Cited by 5 publications

(1 citation statement)

References 35 publications

(39 reference statements)

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“…One of the main routes for the dissemination of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) is the aquatic environment [36,37]. Unfortunately, conventional water treatment processes cannot entirely eliminate the determinants of resistance to AB from raw water, and for this reason the water bodies represent an important source of ARG dissemination [38]. Consequently, wastewater reused for irrigation not only in the rice fields, but also in the surrounding agricultural land, can have a return in the cycle and reach animals and humans through the food chain [39].…”

Section: Phenotypic Characterization Of Antibiotic Resistancementioning

confidence: 99%

Evaluation of the Microbiological Quality of Water in the Rice-Growing System of the Lis Valley, Portugal

et al. 2022

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Society’s demand for the preservation of water resources has stimulated technological advances and improved agricultural management, to limit its negative impacts, favor socio-economic development and promote the conservation of natural resources. This study assessed the impact of a rice farming system in the Mediterranean area on the microbiological quality of water, representing the irrigation and cultivation conditions of the Lis Valley Irrigation District (LVID). Indicators used include the counting of total coliforms (TC), fecal coliforms (FC), fecal enterococci (FE) and ampicillin-resistant Enterobacteriaceae (Ampr), and a phenotypic characterization of antibiotic (AB) resistances. The results show that the water at the LVID inlets contained high microbiological contamination due to sources outside the perimeter. The rice paddy agroecosystem had a beneficial impact on the load of TC, FC and FE of the respective drainage water, with a decrease of two orders of magnitude between the values of these counts at the inlets and at the outlets of the rice fields. However, the relative frequency of Enterobacteriaceae Ampr increased in the rice paddy soil, possibly as a result of the favorable conditions provided by this wetland ecosystem for the transfer of AB resistance genes. The analysis of AB resistance patterns revealed high relative resistance percentages for both β-lactams and non-β-lactams in all samples. This study made it possible to assess the risks of microbiological contamination of irrigation water and the beneficial effect of the rice-growing system in controlling the applied indicators.

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Section: Phenotypic Characterization Of Antibiotic Resistancementioning

confidence: 99%

Evaluation of the Microbiological Quality of Water in the Rice-Growing System of the Lis Valley, Portugal

et al. 2022

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Occurrence and risk assessment of pharmaceuticals in hospital wastewater in Costa Rica

et al. 2023

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Hospital sewage in Brazil: a reservoir of multidrug-resistant carbapenemase-producing Enterobacteriaceae

Gonçalves,

Chang,

Nobrega

et al. 2024

Braz. J. Biol.

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The One Health concept recognizes that human health is clearly linked to the health of animals and the environment. Infections caused by bacteria resistant to carbapenem antibiotics have become a major challenge in hospitals due to limited therapeutic options and consequent increase in mortality. In this study, we investigated the presence of carbapenem-resistant Enterobacteriaceae in 84 effluent samples (42 from hospital and 42 from non-hospital) from Campo Grande, midwest Brazil. First, sewage samples were inoculated in a selective culture medium. Bacteria with reduced susceptibility to meropenem and ertapenem were then identified and their antimicrobial susceptibility was determined using the Vitek-2 system. The blaKPC genes were detected using PCR and further confirmed by sequencing. Carbapenem-resistant Enterobacteriaceae (CRE) were identified in both hospital (n=32) and non-hospital effluent (n=16), with the most common being Klebsiella pneumoniae and of the Enterobacter cloacae complex species. This is the first study to indicate the presence of the blaKPC-2 gene in carbapenem-resistant Enterobacteriaceae, classified as a critical priority by the WHO, in hospital sewage in this region. The dissemination of carbapenem antibiotic-resistant genes may be associated with clinical pathogens. Under favorable conditions and microbial loads, resistant bacteria and antimicrobial-resistance genes found in hospital sewage can disseminate into the environment, causing health problems. Therefore, sewage treatment regulations should be implemented to minimize the transfer of antimicrobial resistance from hospitals.

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The occurrence of antimicrobial residues and antimicrobial resistance genes in urban drinking water and sewage in Southern Brazil

Cited by 5 publications

References 35 publications

Evaluation of the Microbiological Quality of Water in the Rice-Growing System of the Lis Valley, Portugal

Evaluation of the Microbiological Quality of Water in the Rice-Growing System of the Lis Valley, Portugal

Occurrence and risk assessment of pharmaceuticals in hospital wastewater in Costa Rica

Hospital sewage in Brazil: a reservoir of multidrug-resistant carbapenemase-producing Enterobacteriaceae

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