The Occurrence of Amphizoic Amebae in Domestic Animals

Kadlec, V

doi:10.1111/j.1550-7408.1978.tb04403.x

Cited by 20 publications

(10 citation statements)

References 11 publications

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“…Although Fields et al (1989) mentioned temperatures reaching 52ºC, ATCC Hartmannella strains, including one deposited originally as H. thermophila (strain LCD-1A), are cultured at 25ºC. The culturing experiments made by Kadlec (1978) with several Hartmannella vermiformis strains isolated from domestic animals showed that amoebae adapted to room temperatures degenerate upon prolonged culturing at 37ºC. The extreme high temperature tolerance was proved in four Hartmannella strains isolated from hot-water systems by culturing them at 53ºC (Rohr et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The data collected on a variety of bacterial endocytobionts in Hartmannella vermiformis Page, 1967, and the recognition of its special role in the propagation of Legionnaires' disease, provide evidence that these amoebae are more important as hosts of true or potential pathogens of humans than as primary agents of human diseases (Fields et al 1989, Kuchta et al 1993, Brieland et al 1996, Fields 1996, Horn et al 2000. Scarce data available on Hartmannella infections in animals including fish point to a similar situation (Kadlec 1978, Bomhard et al 2002, Telford and Bursey 2003, Dyková and Lom 2004. Nevertheless, pathogenicity of Hartmannella spp.…”

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confidence: 99%

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Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

Dyková¹,

Pindova²,

Fiala³

et al. 2005

FOLIA PARASIT

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Abstract. Based on morphological and molecular characterisation, four amoeba strains isolated from organs of freshwater fish were identified as Hartmannella vermiformis Page, 1967. Small subunit rRNA gene sequences of these strains expand the set of corresponding complete and almost complete sequences of this species to twelve. A new species-specific oligonucleotide probe inferred from recently available SSU rRNA gene sequences was designed and successfully tested in tissue lesions produced by one strain of H. vermiformis in experimentally infected fish.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

Dyková¹,

Pindova²,

Fiala³

et al. 2005

FOLIA PARASIT

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“…These amoebae can survive severe conditions by forming resistant cysts. Some are of medical importance as causative agents of infections and disease in humans (Cerva 1980;Martinez 1985;Ma et al 1990) and animals (Kadlek 1978). Naegleria fowleri produces primary amoebic meningoencephalitis (PAM).…”

Section: Introductionmentioning

confidence: 99%

The identification of free-living environmental isolates of amoebae from Bulgaria

Tsvetkova

Schild

Panaiotov

et al. 2004

Parasitology Research

141

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A survey was carried out in Bulgaria to determine the presence of free-living amoebae (FLA) from environmental sources. In 171 (61.1%) of 280 samples, isolates of Acanthamoeba with group II or III morphology, as well as Hartmannella spp. were recovered. Five isolates named "6" (artificial lake), Ep (lake), G2 (soil), R4* (river) and PK (spring water)--all exhibiting a highly efficient proliferation in axenic cultures--were subsequently cloned and subjected to molecular analyses for identification and genotyping In accordance with morphological findings, PCR-based analyses identified four isolates (6, Ep, G2, R4*) belonging to the genus Acanthamoeba. Confirmation of these findings was obtained by phylogenetic analysis using partial sequencing of the 18S rDNA (ASA.S1) Acanthamoeba-gene. Comparison of these sequences with corresponding regions from other Acanthamoeba strains available from GenBank sorted all four isolates into the sequence type group T4 that contains most of the pathogenic Acanthamoeba strains already identified. The fifth isolate (PK) exhibited morphological characteristics matching those of Hartmannella, and scored negative in the Naegleria fowleri and Acanthamoeba PCRs.

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“…1). Alternatively, it is also plausible that the oxidative DNA glycosylases in Mimivirus were either acquired from or transferred to a higher eukaryote since the mimiviral host, Acanthamoeba, is an opportunistic pathogen in vertebrates including humans [41,42] and the mimiviral Nei proteins share sequence homology to the NEIL proteins in vertebrates (Fig. 1). …”

Section: Origin Of the Oxidative Dna Glycosylases In Mimivirusmentioning

confidence: 99%

Human endonuclease VIII-like (NEIL) proteins in the giant DNA Mimivirus

et al. 2007

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Endonuclease VIII (Nei), which recognizes and repairs oxidized pyrimidines in the Base Excision Repair (BER) pathway, is sparsely distributed among both the prokaryotes and eukaryotes. Recently, we and others identified three homologs of E. coli endonuclease VIII-like (NEIL) proteins in humans. Here, we report identification of human NEIL homologs in Mimivirus, a giant DNA virus that infects Acanthamoeba. Characterization of the two mimiviral homologs, MvNei1 and MvNei2, showed that they share not only sequence homology but also substrate specificity to the human NEIL proteins, that is, they recognize oxidized pyrimidines in duplex DNA and in bubble substrates and as well show 5′2-deoxyribose-5-phosphate lyase (dRP lyase) activity. However, unlike MvNei1 and the human NEIL proteins, MvNei2 preferentially cleaves oxidized pyrimidines in single stranded DNA forming products with a different end chemistry. Interestingly, opposite base specificity of MvNei1 resembles human NEIL proteins for pyrimidine base damages whereas it resembles E. coli formamidopyrimidine DNA glycosylase (Fpg) for guanidinohydantoin (Gh), an oxidation product of 8-oxoguanine. Finally, a conserved arginine residue in the "zincless finger" motif, previously identified in human NEIL1, is required for the DNA glycosylase activity of MvNeil. Thus, Mimivirus represents the first example of a virus to carry oxidative DNA glycosylases with substrate specificities that resemble human NEIL proteins. Based on the sequence homology to the human NEIL homologs and novel bacterial NEIL homologs identified here, we predict that Mimivirus may have acquired the DNA glycosylases through the host-mediated lateral transfer from either a bacterium or from vertebrates.

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The Occurrence of Amphizoic Amebae in Domestic Animals

Cited by 20 publications

References 11 publications

Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

Fish-isolated strains of Hartmannella vermiformis Page, 1967: morphology, phylogeny and molecular diagnosis of the species in tissue lesions

The identification of free-living environmental isolates of amoebae from Bulgaria

Human endonuclease VIII-like (NEIL) proteins in the giant DNA Mimivirus

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