The occurrence and determination of N-Nitroso compounds

Processing of O6-methylguanine into DNA double-strand breaks requires two rounds of replication whereas apoptosis is also induced in subsequent cell cycles

“…One of the DNA lesions that have been identified to activate the DNA damage response is O 6 -methylguanine (O 6 MeG). This damage, induced in DNA by many environmental carcinogens 1 and anticancer drugs such as temozolomide, procarbazine, dacarbazine and streptozotocine, 2 is a highly cytotoxic, apoptotic, mutagenic, recombinogenic and clastogenic DNA adduct. 3 Once induced in the DNA the damage is either repaired by MGMT 4 or becomes converted by replication into the mispair O 6 MeG/T, which gives rise to transition mutations.…”

Novel approaches to improving the chemical safety of the meat chain towards toxicants

“…N-nitroso compounds (National Toxicology Program -Department of Health and Human Services, 2014) in diet mainly come from cured meat products (Bartsch & Montesano, 1984;Fiddler, 1975;Rywotycki, 2007), but as heat-processed toxicants, they can be formed by nitrosation of proline during the frying of pork meat (Walker, 1990). It is also important to note that they can be formed in vivo by simultaneous ingestion of nitrite or nitrogen oxides and a nitrosable substrate such as a secondary amine (Wogan & Tannenbaum, 1975).…”

Oximation and Nitrosation of Adrenalone

“…NMR spectra were obtained with a Hitachi Perkin Elmer R-20 NMR spectrometer using deuterated dimethyl sulfoxide (DMSO-d 6 ) as solvent and tetramethyl sirane (TMS) as an internal standard. Mass spectra were recorded on a Hitachi RMS-4 Mass spectrometer.…”