The occurence of 1-methyladenine in ribonucleic acid

Dunn, David B.

doi:10.1016/0006-3002(61)90668-0

Cited by 176 publications

(110 citation statements)

References 13 publications

Supporting

Mentioning

103

Contrasting

Order By: Relevance

“…The RNA modification m 1 A was discovered many years ago 14 and is prevalent in rRNA and tRNA where it maintains tertiary structure and effects translation. 15,16 m 1 A is found in all regions of transcripts including the coding sequence (CDS), as well as 5’ and 3’ untranslated regions (UTRs).…”

Section: Rna Modificationsmentioning

confidence: 99%

Role of RNA modifications in brain and behavior

Jung

Goldman

2018

Genes Brain and Behavior

View full text Add to dashboard Cite

Much progress in our understanding of RNA metabolism has been made since the first RNA nucleoside modification was identified in 1957. Many of these modifications are found in noncoding RNAs but recent interest has focused on coding RNAs. Here, we summarize current knowledge of cellular consequences of RNA modifications, with a special emphasis on neuropsychiatric disorders. We present evidence for the existence of an “RNA code,” similar to the histone code, that fine-tunes gene expression in the nervous system by using combinations of different RNA modifications. Unlike the relatively stable genetic code, this combinatorial RNA epigenetic code, or epitranscriptome, may be dynamically reprogrammed as a cause or consequence of psychiatric disorders. We discuss potential mechanisms linking disregulation of the epitranscriptome with brain disorders and identify potential new avenues of research.

show abstract

Section: Rna Modificationsmentioning

confidence: 99%

Role of RNA modifications in brain and behavior

Jung

Goldman

2018

Genes Brain and Behavior

View full text Add to dashboard Cite

show abstract

“….for Wheai Efalbryo .YAM-'-: iRNA Methj~ltrnn~eruses Becieuse it is devoid of two of tlae modified nucleotide constituents that are found in eukaryotic transfer RNA, bulk tRNA from E. coli is a relatively good substrate for the SAMt :tRNA methyltransferases in an 52'9 fraction from a wheat embryo homogenate (I I). Accordingly, at pH 7.5 and in the absence of naagnesium ion, there is roughly equivalent incorporation of methyl groups into two principal products, 1 -methyladenylate and N2-dimethylguanylate, both of which are present in wheat embryo tRNA (2) and absent from E. coli tRNA (5,15,16). By manipulating the pH value and magnesiuna ion concentration of the reaction medium, it is possible to suppress synthesis of 1-methyladenylate, and thereby to achieve selective synthesis of N"dirnethylguaany1ate (I 1).…”

Section: Selective Gerwrrriion Qf' N2-ditlacd/g-andg~~~tty/d1~c9mentioning

confidence: 99%

Wheat Embryo Ribonucleates. V. Generation of N²-Dimethylgnanylate When 'Fully Sequenced' Homogeneous Species of Transfer RNA are Used as Substrates for Wheat Embryo Methyltransferases

Kwong¹,

Lane²

1975

Can. J. Biochem.

View full text Add to dashboard Cite

When S-adenosly[methyl-14-C]methionine and various species of transfer RNA are used as substrates for wheat embryo methyltransferases, the principal site of guanylate-N-2 methylation can be shown to be a G-residue between the stems of the dihydrouridine and anticodon loops. This common site of guanylate-N-2 methylation is referred to as the interstem target site. 2. When the interstem target site is the non-terminal G-residue in a G-C-G-C sequence, as in the cases of Escherichia coli tRNA1-Leu, tRNA-Ile, and tRNA3-Ser, there is preponderant dimethylation to yield N-2-dimethylguanylate. 3. When the interstem target site is part of a U-C-G-U sequence, as in the case of E. coli tRNAf-Met, there is diminished dimethylation and correspondingly increased monomethylation to yield N-2-monomethylguanylate. 4. When the interstem target site is the non-terminal G-residue in an A-U-G-G sequence, as in the case of yeast tRNA-Asp, there is negligible dimethylation and almost exclusive monomethylation to yield N-2-monomethylguanylate. 5. The concerted way in which the primary, secondary, and tertiary structures of tRNA molecules might influence the efficacy of these methylations is the subject of a brief discussion. Attention is also focused on the evolutionary and molecular basis for the generally non-random distributions of methylated oligonucleotide sequences in ribosomal and transfer ribonucleates.

show abstract

“…One RNA modification present toward the 5 ′ ends of mRNA transcripts is N 1 -methyladenosine (m 1 A) (Dominissini et al 2016;Li et al 2016), which was initially identified in total RNA and rRNAs (Dunn 1961;Hall 1963;Klootwijk and Planta 1973). Intriguingly, the position of m 1 A modifications has been shown to be more correlated with the position of the first intron than with transcriptional or translational start sites (Fig.…”

Section: Transcripts Harboringmentioning

confidence: 99%

A common class of transcripts with 5′-intron depletion, distinct early coding sequence features, and N¹-methyladenosine modification

Cenik

Chua

Singh

et al. 2016

RNA

View full text Add to dashboard Cite

Introns are found in 5′ untranslated regions (5 ′ UTRs) for 35% of all human transcripts. These 5 ′ UTR introns are not randomly distributed: Genes that encode secreted, membrane-bound and mitochondrial proteins are less likely to have them. Curiously, transcripts lacking 5 ′ UTR introns tend to harbor specific RNA sequence elements in their early coding regions. To model and understand the connection between coding-region sequence and 5′ UTR intron status, we developed a classifier that can predict 5′ UTR intron status with >80% accuracy using only sequence features in the early coding region. Thus, the classifier identifies transcripts with 5 ′ proximal-intron-minus-like-coding regions ("5IM" transcripts). Unexpectedly, we found that the early coding sequence features defining 5IM transcripts are widespread, appearing in 21% of all human RefSeq transcripts. The 5IM class of transcripts is enriched for non-AUG start codons, more extensive secondary structure both preceding the start codon and near the 5 ′ cap, greater dependence on eIF4E for translation, and association with ER-proximal ribosomes. 5IM transcripts are bound by the exon junction complex (EJC) at noncanonical 5 ′ proximal positions. Finally, N 1 -methyladenosines are specifically enriched in the early coding regions of 5IM transcripts. Taken together, our analyses point to the existence of a distinct 5IM class comprising ∼20% of human transcripts. This class is defined by depletion of 5 ′ proximal introns, presence of specific RNA sequence features associated with low translation efficiency, N 1 -methyladenosines in the early coding region, and enrichment for noncanonical binding by the EJC.

show abstract

The occurence of 1-methyladenine in ribonucleic acid

Cited by 176 publications

References 13 publications

Role of RNA modifications in brain and behavior

Role of RNA modifications in brain and behavior

Wheat Embryo Ribonucleates. V. Generation of N²-Dimethylgnanylate When 'Fully Sequenced' Homogeneous Species of Transfer RNA are Used as Substrates for Wheat Embryo Methyltransferases

A common class of transcripts with 5′-intron depletion, distinct early coding sequence features, and N¹-methyladenosine modification

Contact Info

Product

Resources

About

The occurence of 1-methyladenine in ribonucleic acid

Cited by 176 publications

References 13 publications

Role of RNA modifications in brain and behavior

Role of RNA modifications in brain and behavior

Wheat Embryo Ribonucleates. V. Generation of N2-Dimethylgnanylate When 'Fully Sequenced' Homogeneous Species of Transfer RNA are Used as Substrates for Wheat Embryo Methyltransferases

A common class of transcripts with 5′-intron depletion, distinct early coding sequence features, and N1-methyladenosine modification

Contact Info

Product

Resources

About

Wheat Embryo Ribonucleates. V. Generation of N²-Dimethylgnanylate When 'Fully Sequenced' Homogeneous Species of Transfer RNA are Used as Substrates for Wheat Embryo Methyltransferases

A common class of transcripts with 5′-intron depletion, distinct early coding sequence features, and N¹-methyladenosine modification