The Nutritional Requirements of Haemophilus pertussis

Growth Idiosyncrasies of Some Bordetella pertussis Strains, and the Intracerebral Virulence of these Strains in Mice

“…The medium described by Jebb & Tomlinson (1955) was found satisfactory for this work. It was essential to boil the medium during charcoal absorption or to stir efficiently when the process was done in the steamer.…”

“…The storage of organisms, and their recovery from the dried state for the preparation of suspensions, was as previously described (Brown, 1958). In this work all organisms were washed once with saline and resuspended in the medium described by Jebb & Tomlinson (1955). The estimation of total numbers in each suspension was made by opacity measurements as before.…”

The Minimal Amino Acid Requirements of Haemophilus pertussis

“…Because most of the amino acids used by Jebb & Tomlinson (1955) were natural L-isomers it was possible that cystine or cysteine was present as an impurity in one of them, and glutamic acid, being present in the highest concentration (0.01 M), was most suspect. When tested by the cyanide-nitroprusside reaction, the sample of L-glutamic acid which had been used was found to contain traces of cystine.…”

“…Imamura (1952), however, obtained growth with a wider variety of sulphur sources, including some inorganic compounds. The observations of Jebb & Tomlinson (1955), who used small inocula, suggested that cystine itself was not an essential requirement for growth. There are also conflicting results regarding the other amino acid requirements of H. pertussis.…”

“…There are also conflicting results regarding the other amino acid requirements of H. pertussis. Imamura (1952) and Jebb & Tomlinson (1955) showed that H. pertussis would grow in media containing cystine + glutamic acid as the only amino acids. On the other hand, Proom (1955) stated that a t least eight amino acids must be present in a medium if it were to support the growth of H .…”