The Nutrition of Monosporium apiospermum

Wolf, Frederick A.; Bryden, Robert R.; Maclaren, J. A.

doi:10.2307/3755435

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…Although considerable research has been done on A. boydii, one can question the value of conclusions made from data accumulated with a single isolate. Wolf et al (38) demonstrated that a single strain of M. apiospermum was autotrophic for its vitamins, whereas Villela and Cury (37) studied a strain of A. boydii which was biotin deficient and unable to grow at 37 C. In other studies involving more than a single isolate of A. boydii or M. apiospermum (4,5,14), considerable difference has been noted among individual strains with respect to growth and sporulation. Assuming that differences might also exist in the virulence of individual isolates, the pathogenic potential of three strains of A. boydii and three strains of M. apiospermum were examined in this study.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Allescheria boydii for Mice

Lupan

Cazin

1973

Infect Immun

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Allescheria boydii and its imperfect state, Monosporium apiospermum, were studied to determine whether asexual or sexual strains might exhibit different pathogenic potentials for mice. Six different strains of the fungus were inoculated into mice by the intravenous, intracerebral, intraperitoneal, and intranasal routes. Cortisone-treated mice regularly developed infections after inoculation by any of the routes tested. Mice that had not been treated with cortisone were most susceptible to infection by the intravenous route and least susceptible to infection by the intranasal or intraperitoneal route; nevertheless, all animals that did not receive cortisone were considerably more resistant to infection by the fungus than were comparable groups of cortisone-treated animals. Pathogenicity of the fungus appears to be strain dependent and entirely unrelated to its sexual or asexual form. Studies made to determine accurate viable spore counts of the fungus revealed that the highest viable spore count was generally observed using Sabouraud dextrose agar or potato dextrose agar at an incubation temperature of 37 C for a period of 5 days.

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of Allescheria boydii for Mice

Lupan

Cazin

1973

Infect Immun

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“…The purple and orange pigments of strain A376, which were analyzed chemically by Villela and Cury in another paper (1950b), and which were evident in our cultures of this isolate, GORDON are characteristic of Cephalosporium and do not correspond to the "greenish-ochraceous" and "smoky-brown" coloration observed by Shear. In addition, the optimum temperature and pH requirements determined by Villela and Cury (1950a) for their strain differ markedly from those determined by Wolf et al (1950) with their culture of M. apiospermum.…”

Section: Discussionmentioning

confidence: 77%

NUTRITION AND SPORULATION OF ALLESCHERIA BOYDII

Gordon

1957

J Bacteriol

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There have been few reports concerning the nutritional requirements of the pathogenic ascomycete, Allescheria boydii, or of its imperfect form, Monosporium apiospermum. Benham and Georg (1948) found that a strain of A. boydii isolated by Gay and Bigelow (1930) from a case of mycetoma was autotrophic for growth factors. They cultured it on a dextrose agar containing asparagine as the sole nitrogen source and observed formation of fertile perithecia. Wolf et al. (1950) conducted a detailed nutritional study of a single strain of M. apiospermum and found that it grew well in Czapek's solution without the addition of growth factors. These authors also investigated carbon and nitrogen utilization by this mold, as well as pH and temperature requirements. However, de Area Leao and Cury (1950) and Villela and Cury (1950a) reported a biotin deficiency in a strain of A. boydii whose cultural history dated back to the original isolate of Shear (1922). They obtained no growth in a glucose-asparagine medium unless biotin was added. A single strain of M. apiospermum tested by them was found to have no vitamin deficiency. Villela and Cury (1951) subsequently stated that four additional strains of A. boydii tested grew without the addition of biotin. The study here reported originated partly as an attempt to resolve the above discrepancies and also to determine the effect of nutritional variation upon the formation of perithecia and coremia by different strains of A. boydii and M. apiospermum.

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“…Because this enzyme is usually considered to be located intracellularly, it may be that strain 806 lacks a permease for sucrose. Wolf et al (1950) tested only one strain of the organism in their study, and it may be that their single strain had a similar deficiency in its ability to utilize mannitol. As a result of our studies, we feel that these organisms sporulate so well on mannitol that we have begun a study to determine whether, by using this sole carbohydrate in the medium, we can rejuvenate some of our old stock strains which at present produce ascocarps only sparsely.…”

Section: Discussionmentioning

confidence: 99%

“…He also confirmed the results of Benham and Georg (1948) that rich sources of organic nitrogen stimulate the production of conidia, but inhibit the production of ascocarps. Wolf, Bryden, and MacLaren (1950) were the first to demonstrate that M. apiospermum was autotrophic for its vitamins; they also showed that the organism is able to utilize a wide variety of sugars, sugar alcohols, amino acids, and organic acids as carbon sources during a 2-week test period. Furthermore, they found that the organism was nonexacting in its nitrogen requirement, being able to utilize nitrate, ammonium, or amino nitrogen.…”

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confidence: 99%

CARBOHYDRATE NUTRITION AND SPORULATION OF ALLESCHERIA BOYDII

Cazin

Decker

1964

J Bacteriol

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The influence of various carbohydrates on the growth and sporulation of Allescheria boydii was studied by incorporating the test substance in a medium consisting of the inorganic salts mixture contained in Czapek Dox medium with 2% agar. The data indicate that a wide variety of carbohydrates serve adequately as the sole source of carbon for A. boydii, and for its imperfect form, Monosporium apiospermum. Of those compounds tested, only raffinose and inulin were not assimilated by any of the organisms; one strain differed from all others tested in not being able to utilize sucrose. Of the ascigerous strains tested, those maintained in stock culture for a long period of time were able to grow somewhat more profusely on the various carbohydrates; recently isolated strains produced ascocarps in greater abundance. In contrast to reports appearing in the literature, these organisms are capable of assimilating mannitol, maltose, and lactose.

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The Nutrition of Monosporium apiospermum

Cited by 7 publications

References 10 publications

Pathogenicity of Allescheria boydii for Mice

Pathogenicity of Allescheria boydii for Mice

NUTRITION AND SPORULATION OF ALLESCHERIA BOYDII

CARBOHYDRATE NUTRITION AND SPORULATION OF ALLESCHERIA BOYDII

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