The nutrition of <i>Staphylococcus aureus</i>; nicotinic acid and vitamin B1

Knight, B. C. J. G.

doi:10.1042/bj0310731

Cited by 118 publications

(32 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The specificity of nicotinic acid in the nutrition of pea roots is in substantial agreement with the specificity of this substance in the nutrition of Staphylococcus aureus (7,8,9), dysentery bacillus (5,6) and. the dog (11), with the particular exception that ,B-picoline is claimed to possess curative activity for black tongue.…”

Section: Discussionsupporting

confidence: 55%

Specificity of Nicotinic Acid as a Growth Factor For Isolated Pea Roots1

Bonner

1940

Plant Physiology

View full text Add to dashboard Cite

It is known that nicotinic acid is essential to the growth of numerous species of isolated roots (ADDICOTT and BONNER, 1, BONNER and DEVIRIAN, 4). The present paper is concerned with the chemical specificity of nicotinic acid as a root growth factor. Methods The pea roots (variety Perfection) which were used exclusively in the present experiments were cultured according to the methods outlined in earlier papers (BONNER and ADDICOTT,3; BONNER and DEVIRIAN, 4). Fourmm. tips were cut from the roots of aseptic pea seedlings and transferred to nutrient medium containing per liter of redistilled water 236 mg.Ca (NO3)2 4H20; 36 mg. MgSO4 7H20; 81 mg. KNO3; 65 mg. KCl; 20 mg. KH2P04; 1.5 mg. ferric tartrate; and 40 gm. of sucrose. After the tips had remained in the medium for one week at 250 C., and had grown into roots several cm. long, they were subcultured by tranferring 1-cm. tips to fresh nutrient solution. This process of subculture was repeated weekly. Vitamin B1, which is essential to the continued growth of isolated pea roots, was added at the rate of 0.1 mg. per liter to all of the nutrient solution for transfers later than the first. Nicotinic acid, or related substance to be tested for ability to replace nicotinic acid, was added at the rate of 0.5 mg. per liter, sinee this concentration has been found to be approximately optimal for pea roots (ADDICOTT and DEVIRIAN, 2).A rigid selection for uniformity in root growth rate was carried out at the end of the first week of culture in order to assure the greatest possible uniformity in the roots actually used in each experiment. In a single experiment 400-500 roots were cultured and 20-50 roots were used for testing the activity of each substance reported below. In each experiment the roots were maintained through 5 or more weekly transfers. ExperimentationThe ability of decreasing concentrations of nicotinic acid to support the continued growth of isolated pea roots is shown in figure 1. Although 0.5 y nicotinic acid per liter of nutrient solution supports modest growth, a ten times higher concentration is needed to support growth at a significant level. Still ten times higher concentration (50 y per liter) supports growth

show abstract

Section: Discussionsupporting

confidence: 55%

Specificity of Nicotinic Acid as a Growth Factor For Isolated Pea Roots1

Bonner

1940

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…The niacin auxotrophy is due to a lack of nicotinate-nucleotide diphosphorylase (EC 2.4.2.19). These auxotrophies have been experimentally documented for S. aureus in the past (33).…”

Section: Known S Aureus Virulence Factors Are Unequally Distributed mentioning

confidence: 99%

“…These models lacked the ability to synthesize additional compounds including the nucleotide guanine and the vitamin riboflavin as well as the amino acids leucine, arginine, histidine, tryptophan, phenylalanine, methionine, proline, and tyrosine (Table 1). Previous work has demonstrated that some strains of S. aureus are able to grow in minimal media devoid of amino acids after a long period of training or weaning away from amino acid addition (33,34). We experimentally confirmed that four strains (USA300, N315, 8325, and Mu50) could grow in minimal media supplemented with proline, serine leucine, threonine, thiamin, and niacin (Fig.…”

Section: Known S Aureus Virulence Factors Are Unequally Distributed mentioning

confidence: 99%

Comparative genome-scale modelling of Staphylococcus aureus strains identifies strain-specific metabolic capabilities linked to pathogenicity

Bosi

Monk

Aziz

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

219

196

View full text Add to dashboard Cite

Staphylococcus aureus is a preeminent bacterial pathogen capable of colonizing diverse ecological niches within its human host. We describe here the pangenome of S. aureus based on analysis of genome sequences from 64 strains of S. aureus spanning a range of ecological niches, host types, and antibiotic resistance profiles. Based on this set, S. aureus is expected to have an open pangenome composed of 7,411 genes and a core genome composed of 1,441 genes. Metabolism was highly conserved in this core genome; however, differences were identified in amino acid and nucleotide biosynthesis pathways between the strains. Genome-scale models (GEMs) of metabolism were constructed for the 64 strains of S. aureus. These GEMs enabled a systems approach to characterizing the core metabolic and panmetabolic capabilities of the S. aureus species. All models were predicted to be auxotrophic for the vitamins niacin (vitamin B3) and thiamin (vitamin B1), whereas strain-specific auxotrophies were predicted for riboflavin (vitamin B2), guanosine, leucine, methionine, and cysteine, among others. GEMs were used to systematically analyze growth capabilities in more than 300 different growth-supporting environments. The results identified metabolic capabilities linked to pathogenic traits and virulence acquisitions. Such traits can be used to differentiate strains responsible for mild vs. severe infections and preference for hosts (e.g., animals vs. humans). Genome-scale analysis of multiple strains of a species can thus be used to identify metabolic determinants of virulence and increase our understanding of why certain strains of this deadly pathogen have spread rapidly throughout the world.systems biology | mathematical modeling | pathogenicity | core genome | pangenome

show abstract

“…The second thing was quite different and it was that someone had shown that compounds that were known to be vitamins for man were also growth factors for certain bacteria (Knight, 1937). I was interested in vitamins as well as enzymes and particularly interested in the role of vitamins as coenzymes.…”

mentioning

confidence: 99%

Adaptation The Fifteenth Marjory Stephenson Memorial Lecture

Clarke

1981

Microbiology

View full text Add to dashboard Cite

The nutrition of Staphylococcus aureus; nicotinic acid and vitamin B1

Cited by 118 publications

References 4 publications

Specificity of Nicotinic Acid as a Growth Factor For Isolated Pea Roots1

Specificity of Nicotinic Acid as a Growth Factor For Isolated Pea Roots1

Comparative genome-scale modelling of Staphylococcus aureus strains identifies strain-specific metabolic capabilities linked to pathogenicity

Adaptation The Fifteenth Marjory Stephenson Memorial Lecture

Contact Info

Product

Resources

About