Toxoplasma gondii
infects most warm-blooded animals, including humans. Felids can serve as both intermediate and definitive hosts for
T. gondii
. However, there is no direct evidence to prove the caracal (
Caracal caracal
) is an intermediate host for
T. gondii
. Here, we report
T. gondii
infection in two caracals in a zoo from China. Antibodies against
T. gondii
were found in both caracals by modified agglutination test (MAT) (cut-off titer: 1:25). Tissue cysts were observed in the leg and tongue muscles of caracal case
#
1. These cysts were confirmed as
T. gondii
by immunohistochemical staining and
T. gondii
was detected by polymerase chain reaction (PCR). Viable
T. gondii
strain was isolated from the striated muscles of caracal case
#
2 and designated as TgCaracalCHn1. DNA from tachyzoites obtained from cell cultures was characterized by RFLP-PCR using ten markers (
SAG1, SAG3, SAG2, BTUB, c22-8, GRA6, c29-2, PK1, L358,
and
Apico
) and the virulence genes (
ROP5
and
ROP18
). The results indicate that this isolate belongs to ToxoDB genotye #2 (Type III). The virulence of this isolate was evaluated in BALB/c mice. A dose of 10
4
TgCaracalCHn1 tachyzoites was non-lethal to mice. Tissue cysts were found in brain tissues of infected mice. This result confirmed that the TgCaracalCHn1 is non-virulent to mice. Current study documents first isolation of viable
T. gondii
strain from caracal and also indicates that caracal can act as new intermediate host for
T. gondii
.