The number of oocytes and the rate of atresia in unilaterally ovariectomized mice up to 8 months after surgery

Baker, T. G.; Challoner, S; Burgoyne, Paul S.

doi:10.1530/jrf.0.0600449

Cited by 18 publications

(8 citation statements)

References 21 publications

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“…It is concluded that growth in length of the uterus occurs during the implantation reaction and this may assist in the accommodation of blastocysts along the length of the uterus. [10] A single horn of the mice uterus is provided for the development of as many as 12 young pups, after 3 weeks[11] and the single remaining ovary in unilaterally ovariectomized animals (assuming the number of corpora lutea to be an index of ovulation) produces as many ova as both ovaries in normal females.…”

Section: Discussionmentioning

confidence: 99%

Ovulation and rate of implantation following unilateral ovariectomy in mice

Bhattacharya

2013

J Hum Reprod Sci

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OBJECTIVES:Removal of an ovary of any side of the body is called the unilateral ovariectomy (ULO). ULO models are used to analyze the existence of functional and physiological asymmetries between the ovaries, including the ovum implantation.MATERIALS AND METHODS:ULO was done in mice without any specification on their stages of the estrous cycle. The animals were mated between 3rd and 15th day after ULO to observe the consequence of ULO on ovulation and ovum implantation.RESULTS:Ovulation and number of implantation were decreased in proportion to single contralateral ovary if the animals were mated within 36 hrs of ULO. It resulted in increased weight of contralateral ovary due to hypertrophy, and also in compensatory ovulation, formation of corpus luteum (CL) that reached a peak after 14th and 15th day of ULO (sacrificed on 5th day of pregnancy i.e., 19th and 20th day of ULO) compared to animals undergone sham-surgery. Numbers of implantations were also increased progressively on the contralateral ovarian side and get to a peak after similar days of compensation.DISCUSSION AND CONCLUSION:Following ULO, the remaining ovary started to compensate within 5th to 6th day of ULO even during pregnancy. Ovarian histology also showed increased formation of CL which is a marker of rate of ovulation.

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Section: Discussionmentioning

confidence: 99%

Ovulation and rate of implantation following unilateral ovariectomy in mice

Bhattacharya

2013

J Hum Reprod Sci

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“…At this age ‘growing oocytes’ (in stage II-VI follicles) have a single nucleolus that can be used as a counting marker; ‘pool oocytes’ (in stage I follicles) still may have multiple nucleoli and were counted when the nucleus was judged to be cut near the middle (Baker et al, 1980; Jones and Krohn, 1961). …”

Section: Methodsmentioning

confidence: 99%

The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

Vernet

Szot

Mahadevaiah³

et al. 2014

Development

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Outbred XYSry- female mice that lack Sry due to the 11 kb deletion Srydl1Rlb have very limited fertility. However, five lines of outbred XYd females with Y chromosome deletions YDel(Y)1Ct-YDel(Y)5Ct that deplete the Rbmy gene cluster and repress Sry transcription were found to be of good fertility. Here we tested our expectation that the difference in fertility between XO, XYd-1 and XYSry- females would be reflected in different degrees of oocyte depletion, but this was not the case. Transgenic addition of Yp genes to XO females implicated Zfy2 as being responsible for the deleterious Y chromosomal effect on fertility. Zfy2 transcript levels were reduced in ovaries of XYd-1 compared with XYSry- females in keeping with their differing fertility. In seeking the biological basis of the impaired fertility we found that XYSry-, XYd-1 and XO,Zfy2 females produce equivalent numbers of 2-cell embryos. However, in XYSry- and XO,Zfy2 females the majority of embryos arrested with 2-4 cells and almost no blastocysts were produced; by contrast, XYd-1 females produced substantially more blastocysts but fewer than XO controls. As previously documented for C57BL/6 inbred XY females, outbred XYSry- and XO,Zfy2 females showed frequent failure of the second meiotic division, although this did not prevent the first cleavage. Oocyte transcriptome analysis revealed major transcriptional changes resulting from the Zfy2 transgene addition. We conclude that Zfy2-induced transcriptional changes in oocytes are sufficient to explain the more severe fertility impairment of XY as compared with XO females.

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“…The sections were stained with hematoxylin and eosin and analyzed under a light microscope (Olympus, Tokyo, Japan; type CH2). The nucleoli point counting method of Mandel and Zuckerman ('50,'51) modified by Baker et al (1980) was used to calculate the number of oocytes in the analyzed females. In this method each oocyte (all oocytes except class I and II) with a clearly visible nucleolus is scored.…”

Section: Histological Analysis Of the Ovaries And Kidneysmentioning

confidence: 99%

Changes in germ cells population and in vitro oocyte maturation in female mice with imperforated vagina

Lenartowicz¹,

Brzęk²,

Polanski³

et al. 2008

J. Exp. Zool.

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This study characterized female mice with a congenital defect in their reproductive tract. In females derived from an outbred colony maintained in the Department of Genetics and Evolution, the frequency of the imperforated vaginae was approximately 2%. A consequence of this defect is infertility. Affected animals developed hydrometrocolpos (the uterus and vagina were greatly distended by fluid). Morphology of the ovary and oviduct was normal and similar to that of control mice. In the females with an imperforated vagina numerous corpora lutea were observed. Accordingly, we have found that both spontaneous as well as exogenous gonadotrophin-induced ovulation occurs in such females. Nevertheless, oocytes derived from ovaries of occluded females exhibited a partial block during in vitro maturation. Histological analysis of ovarian tissue revealed an increase in the number of primary follicles (type I follicles) and a decrease in the number of secondary and antral follicles (type IV and type VI follicles) when compared with control mice. The concentration of androgens in the ovarian tissue was higher in the affected females. Our data show that females with an imperforated vagina can be a useful model for studying the mechanism of genetic control of the development of the urogenital tract in mammals.

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The number of oocytes and the rate of atresia in unilaterally ovariectomized mice up to 8 months after surgery

Abstract: Summary. Random

Cited by 18 publications

References 21 publications

Ovulation and rate of implantation following unilateral ovariectomy in mice

Ovulation and rate of implantation following unilateral ovariectomy in mice

The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

Changes in germ cells population and in vitro oocyte maturation in female mice with imperforated vagina

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