The Number of False Mounting Affects The Quality of Semen in Bulls

Şahin, Derya

doi:10.46897/lahaed.701388

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…In addition, discarding the pre-sperm fraction as in boar semen collection (Goldberg et al 2013 ) could have an effect on the bacterial load. Furthermore, allowing the male to make several false mounts before collecting the semen, as in bull semen collection (Şahin et al, 2020 ) should, theoretically, help to flush bacteria out of the urethra before the ejaculate is collected. However, this practice is seldom used when collecting stallion semen.…”

Section: Discussionmentioning

confidence: 99%

Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing

Malaluang,

Niazi,

Guo

et al. 2024

Vet Res Commun

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The microbiome plays a significant role in shaping the health and functioning of the systems it inhabits. The seminal microbiome of stallions has implications for the health of the reproductive tract, sperm quality during preservation and antibiotic use in semen extenders. Diverse bacteria are present on the external genital tract and a mix of commensal microorganisms populates various parts of the reproductive tract, influencing the seminal bacterial content. Other sources of bacteria include the environment, semen collection equipment, and personnel. The bacterial load can adversely affect sperm quality and fertility, particularly in artificial insemination, where semen is extended and stored before use. Antibiotics are frequently used to inhibit bacterial growth, but their effectiveness varies depending on the bacterial strains present. The aim of this study was to assess the bacterial diversity in semen from 37 healthy stallions across three European nations (Germany, Portugal, and Sweden) using 16S sequencing. Semen samples were collected from individual stallions at three AI centers; DNA extraction, sequencing, and bioinformatic analysis were performed. Differences in bacterial diversity among the stallions were seen; although bacterial phyla were shared across the regions, differences were observed at the genus level. Climate, husbandry practices, and individual variability likely contribute to these differences. These findings underscore the importance of tailoring antibiotic strategies for semen preservation based on regional bacterial profiles. The study presents a comprehensive approach to understanding the intricacies of the stallion seminal microbiome and its potential implications for reproductive technologies and animal health.

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Section: Discussionmentioning

confidence: 99%

Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing

Malaluang,

Niazi,

Guo

et al. 2024

Vet Res Commun

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“…12.2. software version was used to perform the analysis of motility. Sperm motility features were assessed at 30 frames/s and 60 Hz; spermatozoa with straight line velocity (VSL) 70% and average path velocity (VAP) 50 μm/s were classified as progressive by using an objective at 37°C and 10× magnification and using a 5 μL post‐thawed semen sample on a prewarmed 20 μm‐deep semen analysis Leja 4‐chamber slide (Leja) (Şahi̇n et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Reaction time as a libido indicator and its relation to pre‐freeze and post‐thaw sperm quality in bulls

KORKMAZ

Baştan

Şahin

et al. 2023

Reprod Domestic Animals

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Libido and sperm quality output relationship is already not clear in farm animals. The present study compared reaction time (RT) as a libido indicator and the pre‐freeze and post‐thaw sperm quality of AI bulls. Before the collection of ejaculates (n = 53, from 22 AI bulls [4.2 ± 1 years of age]), RTs were collected using a chronometer as the interval between the bull's arrival at the semen collection area and his first false mount (FM) on another male. The ejaculates were examined for their volume, concentration and motility. Subsequently, all aliquots were diluted with a commercial semen extender and equilibrated for 3 h before freezing. Frozen semen samples were thawed and examined for sperm kinematics using CASA, plasma membrane and acrosome integrity of sperm (PMAI) by flow cytometry. Additionally, the temperature humidity index (THI) values were assessed during the study. Multiple linear regression analysis was used to analyse the data. The results indicated that THI had a significant effect on libido (p < .001). However, libido had no effect on either pre‐ or post‐thaw sperm quality parameters except for the velocity of the average pathway (VAP) (p < .05). Therefore, relying solely on RT –libido‐ as an indicator of bull sperm quality at AI stations may not be reliable, as it is a complex behavioural assessment.

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“…Other kinematics values, namely VAP (µm/s), VSL (µm/s), curvilinear velocity (VCL, µm/s), linearity [LIN = (VSL/ VCL) × 100], straightness [STR = (VSL/VAP) × 100], the amplitude of lateral head displacement (ALH, µm), and beat-cross frequency (BCF, Hz), were also evaluated and expressed with their units. Approximately 400 spermatozoa per sample were evaluated in five microscopic fields (5,28).…”

Section: Semen Collection and Dilutionmentioning

confidence: 99%

First dose optimization study on freezing Anatolian buffalo semen

BAŞTAN,

ŞAHİN,

KORKMAZ

et al. 2023

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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The main objective of sperm production centers is to produce as many straws as possible from the obtained ejaculates using the optimal dilution rate. To this end, this study is the first to evaluate the effect of different semen extender rates on Anatolian buffalo semen quality. Ejaculates were collected by artificial vagina from three Anatolian buffalo bulls (n=105). These ejaculates were divided into three aliquots and filled into 0.25 ml straws with soy-based extenders at the concentrations of 35, 25, and 15 million spermatozoa/straw. The straw samples of different spermatozoa concentrations were frozen. The quality of spermatozoa was evaluated after thawing (37 °C, 30 sec) and following the thermoresistance test (37 °C, 3 h). The post-thaw total motility and progressive motility values were similar between the groups. However, following the thermoresistance test, there was a significant decrease in total motility in the 35 million spermatozoa/straw group, and the progressive motility was significantly higher in the 25 million spermatozoa/straw group. There was no statistically significant difference between the groups in terms of sperm kinetic parameters, except VSL after thawing except the VAP and LIN values following the thermoresistance test. In conclusion, it is recommended to dilute the Anatolian buffalo semen at a concentration of 25 or 100 million spermatozoa/ml per straw when freezing it with a soy-based semen extender. In addition, it is considered that soy-based extenders compensate for cryo-damage to sperm motility for a short time, and the thermoresistance test should be applied for objective evaluation in dose optimization studies.

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The Number of False Mounting Affects The Quality of Semen in Bulls

Cited by 4 publications

References 29 publications

Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing

Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing

Reaction time as a libido indicator and its relation to pre‐freeze and post‐thaw sperm quality in bulls

First dose optimization study on freezing Anatolian buffalo semen

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