The nucleotide sequences of the 5 S rRNAs of seven moids and a yeast and their use in studying ascomycete phylogeny

Abstract

The sequences of the 5 S rRNAs isolated from 8 ascomycete species belonging to the genera Aspergillus, Penicillium, Acremonium and Candida are reported. Two of the examined strains each yielded a mixture of 3 slightly different 5 S RNAs, which were individually sequenced after fractionation. A previously published sequence for Aspergillus nidulans 5 S RNA was found to contain errors. Reconstruction of an evolutionary tree based on 5 S RNA sequences showed that the 16 presently examined ascomycetes form three clusters. The same threefold partition can be observed in the secondary structure pattern, each cluster showing a slightly different variant of the general 5-helix model for 5 S rRNA (De Wachter, Chen and Vandenberghe (1982) Biochimie 64, 311-329), and different sets of secondary structure equilibrium forms in helices C and E of the aforementioned model.

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

“…thaliana minor 5S RNAs differ from the major 5S RNA by one or two base substitutions, as has been described for Escherichia coli (Jarry and Rosset, 1971), and we found many different 5S RNA, as reported in Neurospora (Chen et al 1984). However, only in Escherichia coli, Xenopus, and Neurospora (Selker et al 1985), has it been shown that heterogeneous 5S RNA exist in polysomes.…”

“…In addition to the major 5S RNA species, minor RNA species represent 18% of total 5S RNA. Heterogeneity in 5S RNA has also been reported for a wide range of organisms including bacteria (Jarry and Rosset, 1971), fungi (Wildelman and Nazar, 1982;Chen et al 1984), echinoderms (Lu et al 1980), arthropods (Komiya et al 1980), amphibia (Ford and South- …”

“…Heterogeneity of 5S RNA transcripts has been reported for a wide range of organisms including bacteria (Jarry and Rosset, 1971), fungi (Wildeman and Nazar, 1982;Chen et al 1984), echinoderms (Lu et al 1980), arthropods (Komiya et al 1980), amphibia (Ford and Southern, 1973), birds, and mammals (Ro-Choi et al 1971). Furthermore, in Escherichia coli, Xenopus, and Neurospora it is known that heterogeneous 5S RNA exist in ribosomes.…”

Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.

“…This pattern differed strikingly, in several instances, from that observed in a compilation of genes from the single-celled ascomycete S. cerevisiae (5); differences in codon preference were most evident in Glu, Gln, Cys, Pro, and Arg codons. We speculate that these differences may reflect evolutionary distance between the yeasts and the filamentous ascomycetes (13).…”

A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the a1-alpha 2 repressor.

“…C. albicans has been placed in the group Fungi Imperfecti, the group of fungi that are not known to have arty mating phase. However, sequence analyses of small ribosomal subunit RNAs and 5S rRNA reveal that C. albicans belongs to the order Endomycetales, to which the family Saccharomycetaceae also belongs (7,18). Significant degrees of DNA sequence homology between a number of C. albicans genes and the corresponding S. cerevisiae genes have been reported (2,36,44).…”