The Nucleotide Sequence of Tomato Black Ring Virus RNA-2

Abstract

SUMMARYThe sequence of the 4662 nucleotides [excluding poly(A)] of RNA-2 of tomato black ring virus (TBRV) has been determined. Most of the sequence (4074 nucleotides) encodes a polypeptide of mol. wt. 150000 (150K). The 5' and Y non-coding sequences are 287 and 301 nucleotides in length, differ from the coding sequence in base composition, and contain repeated sequences, some of which resemble oligonucleotides in the 3' non-coding sequence of M-RNA of cowpea mosaic virus (CPMV). From its amino acid composition, the coat protein of TBRV was tentatively located in the Cterminal third of the 150K polypeptide. The amino acid sequence of the 150K polypeptide immediately N-terminal to the putative coat protein sequence was found to resemble parts of the 30K polypeptides of tobamoviruses and, to a lesser extent, part of the 105K polypeptide translation product of CPMV M-RNA.

The Complete Nucleotide Sequence of Plum Pox Virus RNA

“…70 to 90 nucleotides upstream of the poly(A) tail in TVMV, TEV, PeMV and PPV-NAT may also represent a polyadenylation signal as demonstrated for yeasts, where this region is located 30 to 40 nucleotides in front of the poly(A) tail (Zaret & Sherman, 1982). This sequence was also found in the 3' non-coding region of tomato black ring virus RNA-1 (Greif et al, 1988) and RNA-2 (Meyer et al, 1986), and CPMV (van Wezenbeek et al, 1983;Lomonossoff & Shanks, 1983); these viruses also contain plus-sense, monocistronic polyadenylated RNAs.…”

Nucleotide sequence of tomato ringspot virus RNA-2

“…Further analysis was performed using programs and databases available through the Canadian National Research Council's Scientific Numeric Database Service on a DEC VAX. We would like to thank Bill Ronald and Jim Purves of Agriculture Canada for writing a program used to test the goodness of fit between the amino acid composition of the TomRSV coat protein (Tremainc & StaceSmith, 1968) and the calculated amino acid composition of segments of the deduced polyprotein sequence encoded by RNA-2 (see Meyer et aL, 1986). The raspberry isolate of TomRSV used by Tremaine & StaceSmith for the coat protein amino acid composition analysis and the raspberry isolate we have sequenced were both obtained from the British Columbia Frazer Valley growing area and are therefore probably very similar.…”

“…The 5' non-coding region of TomRSV RNA-2 was analysed and compared to the corresponding regions of the two RNA components of TBRV (Meyer et al, 1986;Greif et al, 1988) and GCMV Le Gall et al, 1989), RNA-2 of GFLV (Serghini et al, 1990) and the B and M components of the comovirus CPMV (Lomonossoff & Shanks, 1983;van Wezenbeek et al, 1983). It has been reported previously that TBRV, GCMV, GFLV and CPMV share a conserved UGAAAAAU sequence downstream from the 5' terminus (Serghini et al, 1990).…”

Nucleotide sequence of the coat protein genes of strawberry latent ringspot virus: lack of homology to the nepoviruses and comoviruses

“…Although most of the nepoviruses and comoviruses have 3' UTRs shorter than that of SLRV RNA-2 [nepoviruses, TBRV, 301 nt (Meyer et al, 1986), GCMV, 251 nt (Brault et al, 1989), GFLV, 212 nt (Serghini et al, 1990) and RRV, 397 nt (Blok et al, 1992); comoviruses, RCMV, 263 nt (Shanks et al, 1986), CPSMV, 471 nt (Chen& Bruening, 1992), CPMV, 179 nt (van Wezenbeek et al, 1983), BPMV, 455 nt (MacFarlane et al, 1991)], TomRSV (Rott et al, 1991) has a significantly longer 3' UTR (1550 nt).…”