The nucleotide sequence of the right-hand terminus of adenovirus type 5 DNA: Implications for the mechanism of DNA replication

Steenbergh, P. H.; Sussenbach, J.S.

doi:10.1016/0378-1119(79)90071-4

Cited by 31 publications

(12 citation statements)

References 36 publications

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“…Dinucleotides G-G, T-A, T-G, and T-T were found to be the "strongest" contributors to-the variations. Summing the autocorrelation functions for only these dinucleotides for 32 sequences, we obtained a distribution (Fig. 4) in which the distances scored extend up to 100 bases and some additional small peaks are seen-for example, at about 54, 72, 84, and 93-96 bases.…”

Section: Methods and Resultsmentioning

confidence: 99%

“…A correlation analysis of available sequences of eukaryotic DNAs and mRNAs (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26) as well as of some viral DNAs (27)(28)(29)(30)(31)(32)(33)(34)(35) that are known to be folded in chromatin-like structures (36,37) shows that some of the dinucleotides indeed exhibit this periodicity, although it is well hidden. The period is estimated to be 10.5 + 0.2 bases, which is consistent with recent measurements and estimations of the pitch of DNA in dilute solution and in chromatin (6,38).…”

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confidence: 99%

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The pitch of chromatin DNA is reflected in its nucleotide sequence.

Trifonov¹,

Sussman²

1980

Proc. Natl. Acad. Sci. U.S.A.

525

299

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Section: Methods and Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

The pitch of chromatin DNA is reflected in its nucleotide sequence.

Trifonov¹,

Sussman²

1980

Proc. Natl. Acad. Sci. U.S.A.

525

299

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“…Each end of the viral genome carries an identical sequence of 103 bp (25,35), called the inverted terminal repetition (ITR). The origin of replication has been localized to sequences lying within the ITR (reviewed in reference 4).…”

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confidence: 99%

Rescue of functional replication origins from embedded configurations in a plasmid carrying the adenovirus genome.

Hanahan¹,

Gluzman²

1984

Mol. Cell. Biol.

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A variant of the adenovirus type 5 genome which lacks EcoRI sites has been cloned in a bacterial plasmid after the addition of EcoRI oligonucleotide linkers to its ends. Closed circular forms of the recombinant viral genome were not infectious upon their introduction into permissive eucaryotic cells. The linear genome released by digestion of the 39-kilobase recombinant plasmid (pXAd) with EcoRI produced infectious virus at about 5% of the level of wild-type controls. The viruses which arose were indistinguishable from the parental strain, and the normal termini of the viral genome had been restored. Marker rescue experiments demonstrate that provision of a DNA fragment with a normal viral end improves infectivity. When a small fragment carrying a wild-type left end (the 0 to 2.6% ClaI-B fragment) was ligated to Clal-linearized pXAd, virus was produced with efficiencies comparable to a similar reconstitution of the two ClaI fragments of the wild-type genome. These viruses stably carry the left-end fragment at both ends, leaving the normal right end embedded in 950 base pairs of DNA. The embedded right origin is inactive. The consensus of the analyses reported here is that a free end is a necessary configuration for the sequences which make up the adenovirus origin of replication.Human adenovirus type 5 (AdS) has a double-stranded DNA genome of 36 kilobase pairs (kbp) (33). The 5' ends of the viral DNA are covalently linked to a terminal protein through a deoxycytosine-to-serine phosphoryl bond (5, 23). The 55-kilodalton (kDa) terminal protein is a proteolytic cleavage product of an 80-kDa protein which participates in the initiation of DNA replication (2,4,16,26). The terminal protein precursor forms a complex with a 140-kDa adenovirus-encoded polymerase (15, 27), after which the terminal protein precursor becomes modified through its covalent linkage to a deoxycytosine, which will subsequently become the first nucleotide of the daughter strand. During morphogenesis of the virion, the 80-kDa terminal protein precursor is specifically cleaved by an adenovirus-encoded protease to produce the 55-kDa terminal protein (3,26).Isolation of viral DNA commonly includes a nonspecific proteolytic digestion step (with pronase), which removes all but a few amino acids of the terminal protein from the DNA.

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“…fig.3). The resulting sequence showed a perfect homology with the Ad5 DNA sequence between bp 262 and 63 of the left hand viral end (17) and bp 108 and 250 of the right hand viral end (18), the nucleotides corresponding to the left hand end bp 63 and the right hand end bp 108 being directly linked (Fig.4). No sequences essential for transcription of either early region El or early region E4 appear to be deleted from the linkage site of the viral insertion of 5RK20 cells.…”

Section: Restriction Mappin_ Of_the_viral_dna_containing Clonesmentioning

confidence: 99%

Nucleotide sequence analysis of the linked left and right hand terminal regions of adenovirus type 5 DNA present in the transformed rat cell line 5RK20

et al. 1982

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A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 transformed rat cell line 5RK20 has been cloned in the lambda vector Charon2lA, including the segment containing the linked terminal regions. Sequence analysis of the linkage region showed a perfect homology with the Ad5 DNA sequence and a direct linkage of basepair (bp) 63 of the left hand end of the viral genome to bp 108 of the right hand end. No cellular or rearranged viral sequences were present. Our findings suggest that the joining of the terminal viral regions preceeded the integration of the viral sequences into the cellular genome.

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The nucleotide sequence of the right-hand terminus of adenovirus type 5 DNA: Implications for the mechanism of DNA replication

Cited by 31 publications

References 36 publications

The pitch of chromatin DNA is reflected in its nucleotide sequence.

The pitch of chromatin DNA is reflected in its nucleotide sequence.

Rescue of functional replication origins from embedded configurations in a plasmid carrying the adenovirus genome.

Nucleotide sequence analysis of the linked left and right hand terminal regions of adenovirus type 5 DNA present in the transformed rat cell line 5RK20

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