The nucleotide sequence of mouse OCT-1 cDNA

Степченко, А. Г.

doi:10.1093/nar/20.6.1419

Cited by 37 publications

(20 citation statements)

References 1 publication

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“…VP16 does not activate transcription as effectively in mouse cells as in human cells, probably because the murine Oct-1 homeo domain differs from the human Oct-1 homeo domain at four positions (Goldsborough et al 1990;Stepchenko 1992). As a result, when the human Oct-1 POU domain, but not an Oct-1 POU domain carrying the Oct-2 homeo domain, is expressed in murine NIH-3T3 cells, VP16 activation is stimulated -10-fold, thus establishing that the Oct-1 homeo domain is involved in positive control in vivo (M. Cleary, S. Stern, M. Tanaka, and W. Herr, in prep.…”

Section: A Single Amino Acid Difference Is Sufficient For Selective Pmentioning

confidence: 99%

A single amino acid exchange transfers VP16-induced positive control from the Oct-1 to the Oct-2 homeo domain.

Lai¹,

Cleary²,

Herr³

1992

Genes Dev.

135

125

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The selective association of the herpesvirus trans-activator VP16 with the human Oct-1 homeo domain is a model for differential positive transcriptional control by homeo domains. VP16 discriminates between the closely related homeo domains of Oct-1 and Oct-2 by distinguishing among their seven amino-acid differences; these differences lie on the surface that is thought to be accessible when the homeo domain is bound to DNA. Only two of these seven differences are recognized by VP16, one in each of the first two ~-helices of the tri-~-helical homeo domain. The major determinant for selective association with VP16 in vitro and VP16-induced positive control in vivo is a single glutamic acid residue at position 22 in the first ~-helix of the Oct-1 homeo domain, but the acidic properties of this residue are not critical for association with VP16 in vitro or in vivo, because it can be replaced by glutamine with little or no deleterious effect. Mere replacement of the single corresponding alanine residue in the Oct-2 homeo domain with the key glutamic acid residue is sufficient to confer on the Oct-2 homeo domain the ability to associate with VPI6 in vitro and respond to VP16-induced positive control in vivo. Thus, the specificity of homeo domain positive control can be conferred by a single amino acid difference.

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Section: A Single Amino Acid Difference Is Sufficient For Selective Pmentioning

confidence: 99%

A single amino acid exchange transfers VP16-induced positive control from the Oct-1 to the Oct-2 homeo domain.

Lai¹,

Cleary²,

Herr³

1992

Genes Dev.

135

125

View full text Add to dashboard Cite

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“…As shown in Figure 5A, the murine Oct-1 homeo domain differs from that of h u m a n Oct-1 at four positions (Goldsborough et al 1990;Stepchenko 1992). Two of these differences (E30D and M33L) are at positions that are important for association with VP16 (Stern et al 1989;Lai et al 1992;Pomerantz et al 1992).…”

Section: Murine Oct-1 Associates With Vp16 Less Effectively Than Humamentioning

confidence: 99%

“…This variation can be seen when the human and rodent Oct-1 homeo domains are compared. As illustrated in Figure 5, the murine Oct-1 homeo domain contains four differences with respect to its human counterpart (Goldsborough et al 1990;Stepchenko 1992). Even within rodents the Oct-1 homeo domain varies because a partial rat Oct-1 homeo domain sequence shows that this homeo domain contains the same four murine differences and, in addition, a pair of differences at the amino-terminal end of helix 1 (Lillycrop and Latchman 1991).…”

Section: Implications Of Oct-1 Homeo Domain Variability In Mice and Hmentioning

confidence: 99%

Differential positive control by Oct-1 and Oct-2: activation of a transcriptionally silent motif through Oct-1 and VP16 corecruitment.

Cleary

Stern²,

Tanaka³

et al. 1993

Genes Dev.

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Transcriptional regulation by the ubiquitous human POU homeo domain protein Oct-1 and the related B-cell protein Oct-2 is a model for understanding how proteins that recognize the same regulatory site elicit different programs of gene transcription. Here, we describe a mechanism for differential promoter activation whereby only Oct-l, through selective corecruitment with the herpesvirus trans-activator VPI6, acquires the ability to stimulate transcription from a TAATGARAT-containing site that responds to neither Oct-1 nor Oct-2 alone. To measure differential in vivo activation by human Oct-1 and Oct-2 in response to VP16, we have developed a transient assay in murine NIH-3T3 cells. Surprisingly, murine Oct-1 associates with VP16 much less effectively than its human counterpart, most likely because the murine Oct-1 homeo domain differs at four positions from the human Oct-1 homeo domain. The murine cell transient assay shows directly that human Oct-l, but not human Oct-2, can respond to VP16 in vivo. The Oct-1 DNA-binding POU domain is sufficient and the Oct-1 homeo domain is critical for this response, because an Oct-1 POU domain containing the Oct-2 homeo domain fails to respond to the VP16-induced positive control of transcription. Thus, by selective homeo domain interaction and corecruitment to an otherwise silent regulatory element, VP16 expands the repertoire of sites responsive to Oct-1 without affecting the activity of its close relative Oct-2.

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“…The mouse Chr 1 Committee report (Seldin, 1998), long-range restriction mapping in human and mouse (Oakey et al, 1992), and the human transcript map (Deloukas et al, 1998;Schuler et al, 1996) place several known genes near dr, including POU domain, class 2, transcription factor 1 (Pou2f1/POU2F1) (Jaffe et al, 1995;Stepchenko, 1992); retinoid X receptor gamma (Rxrg/RXRG) (Hoopes et al, 1992); and neurotrophic tyrosine kinase, receptor, related 3 (Ntrkr3/NTRKR3) (Karn et al, 1993;Lai and Lemke, 1994;Murdoch et al, 1997). The human orthologs of these genes lie within the chromosomal region 1q21-q23 (Fig.…”

Section: Assessment Of Candidate Genes and Human Orthologsmentioning

confidence: 99%