The nucleotide sequence of cowpea mosaic virus B RNA

Abstract

The complete sequence of the bottom component RNA (B RNA) of cowpea mosaic virus (CPMV) has been determined. Restriction enzyme fragments of double‐stranded cDNA were cloned in M13 and the sequence of the inserts was determined by a combination of enzymatic and chemical sequencing techniques. Additional sequence information was obtained by primed synthesis on first strand cDNA. The complete sequence deduced is 5889 nucleotides long excluding the 3′ poly(A), and contains an open reading frame sufficient to code for a polypeptide of mol. wt. 207 760. The coding region is flanked by a 5′ leader sequence of 206 nucleotides and a 3′ non‐coding region of 82 residues which does not contain a polyadenylation signal.

Nucleotide Sequence of Barley Yellow Mosaic Virus RNA 1: A Close Evolutionary Relationship with Potyviruses

“…Similar distribution patterns of amino acid matches were observed when the BaYMV 270K protein was compared with the polyproteins of three other potyviruses: tobacco etch (TEV; Allison et al, 1986), tobacco vein mottling (TVMV; Domier et al, 1986) and plum pox viruses (PPV; Maiss et al, 1989). The BaYMV 270K protein as well as the four potyvirus polyproteins displayed few extended matches with the polyproteins encoded by cowpea mosaic virus B component RNA (Lomonossoff & Shanks, 1983), tobacco black ring virus RNA 1 (Greif et al, 1988), poliovirus (Kitamura et al, 1981) or other picornavirus RNAs (Carroll et al, 1984;Palmenberg et al, 1984).…”

The Complete Nucleotide Sequence of Plum Pox Virus RNA

“…70 to 90 nucleotides upstream of the poly(A) tail in TVMV, TEV, PeMV and PPV-NAT may also represent a polyadenylation signal as demonstrated for yeasts, where this region is located 30 to 40 nucleotides in front of the poly(A) tail (Zaret & Sherman, 1982). This sequence was also found in the 3' non-coding region of tomato black ring virus RNA-1 (Greif et al, 1988) and RNA-2 (Meyer et al, 1986), and CPMV (van Wezenbeek et al, 1983;Lomonossoff & Shanks, 1983); these viruses also contain plus-sense, monocistronic polyadenylated RNAs.…”

The nucleotide sequence of parsnip yellow fleck virus: a plant picorna-like virus

“…1), which is in good agreement with an earlier estimate of 9900 + 290 nts (Hemida & Murant, 1989a). Sequence heterogeneity was found at four positions and may have arisen from variation in the virus population, or from errors introduced by reverse transcriptase in the synthesis of the cDNA first strand, as reported by Lomonossoff & Shanks (1983) and Meyer et al (1986). All the variation was located in the large open reading frame (ORF; see below).…”

“…The first AUG codon is probably the initiation codon for the PYFV polyprotein because (i) this AUG has an A residue in position -3 which is unlikely to be skipped by a scanning ribosomal 40S subunit (Kozak, 1987), (ii) the base composition from nts 1 to 278 (34-2% U, 24.8% C, 13.3% G and 27.7% A) has a high U + A and low G residue content consistent with leader sequences in RNA of other plant viruses (Gallie et al, 1987) and if the 5' untranslated region (UTR) is extended to nt 590 the G content rises to 20.0 %; and (iii) an oligopyrimidine tract precedes the first AUG codon. Oligopyrimidine tracts also precede the initiation codons of tomato black ring virus (TBRV) RNA 1 (Greif et al, 1988) and cowpea mosaic virus (CPMV) B RNA (Lomonossoff & Shanks, 1983), and are important in translation initiation of picornavirus RNA (reviewed by Agol, 1991). Collett et al, 1988) and yellow fever flavivirus (YFV; Rice et al, 1985).…”