The Nucleotide Sequence of cDNA Complementary to the C₁ Component of Rat Prostatic Binding Protein

Abstract

The mRNA for component C1 of rat prostatic binding protein has been cloned and characterized. A partially purified mRNA fraction for this complex protein was reverse‐transcribed into double‐stranded cDNA and cloned into the PstI site of plasmid pBR 322. The 426‐base‐pair insert of the recombinant plasmid pC1A75 was completely sequenced. The coding region corresponds precisely to the 88 amino acid residues of C1 and in addition contains the information of a signal peptide of 23 residues. The 5′non‐coding region counts only 19 nucleotides and is incomplete but the 3′‐terminal non‐coding part of 60 nucleotides extends into the poly(A) tail. Sequence analysis of other C1‐positive clones indicates the presence of sequence rearrangements which must have occurred during the cloning procedure. Possible mechanisms for the generation of these cloning artefacts are discussed.

Société Belge De Biochimie Belgische Vereniging Voor Biochemie

“…The most abundant mRNA fraction codes for "prostatic binding protein" (PBP) (HEYNS et al, 1978). cDNA clones for the three PBP mRNA's have been isolated (PARKER et al, 1980;DELAEY et al, 1983) and used as probes for screening a rat genomic library (gift from Dr. T. SARGENT, NIH) in X-phage Charon 4A. PBP can also bind a "proline-rich polypeptide" (PRP), of which there exist several variants (HEYNS et al, 1982).…”