The nucleotide sequence of a major glutamine tRNA from rat liver

Yang, Julia A.; Tai, Lee W.; Agris, Paul F.; Gehrke, Charles W.; Wong, Ting-Kam Leonard

doi:10.1093/nar/11.7.1991

Cited by 17 publications

(6 citation statements)

References 26 publications

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“…This coincides with the presence of Ψ54 in certain tRNAs of animals, and its absence from the tRNAs of bacteria and yeast (Jühling et al 2009). Certain isoacceptors of mammalian tRNAs for Gln, Trp, Pro, Thr, and Arg and of chicken tRNAs for Pro and Trp contain Ψ, instead of T, at position 54 (Harada et al 1975(Harada et al , 1979(Harada et al , 1989Fournier et al 1978;Harada and Nishimura 1980;Yang et al 1983;Keith 1984;Kuchino et al 1987;Harada 1989;Jühling et al 2009).…”

Section: Introductionmentioning

confidence: 54%

The human ortholog of archaeal Pus10 produces pseudouridine 54 in select tRNAs where its recognition sequence contains a modified residue

Deogharia

Mukhopadhyay

Joardar

et al. 2018

RNA

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The nearly conserved U54 of tRNA is mostly converted to a version of ribothymidine (T) in Bacteria and eukaryotes and to a version of pseudouridine (Ψ) in Archaea. Conserved U55 is nearly always modified to Ψ55 in all organisms. Orthologs of TrmA and TruB that produce T54 and Ψ55, respectively, in Bacteria and eukaryotes are absent in Archaea. Pus10 produces both Ψ54 and Ψ55 in Archaea. Pus10 orthologs are found in nearly all sequenced archaeal and most eukaryal genomes, but not in yeast and bacteria. This coincides with the presence of Ψ54 in most archaeal tRNAs and some animal tRNAs, but its absence from yeast and bacteria. Moreover, Ψ54 is found in several tRNAs that function as primers for retroviral DNA synthesis. Previously, no eukaryotic tRNA Ψ54 synthase had been identified. We show here that human Pus10 can produce Ψ54 in select tRNAs, including tRNA Lys3 , the primer for HIV reverse transcriptase. This synthase activity of Pus10 is restricted to the cytoplasm and is distinct from nuclear Pus10, which is known to be involved in apoptosis. The sequence GUUCAm 1 AAUC (m 1 A is 1-methyladenosine) at position 53-61 of tRNA along with a stable acceptor stem results in maximum Ψ54 synthase activity. This recognition sequence is unique for a Ψ synthase in that it contains another modification. In addition to Ψ54, SF9 cells-derived recombinant human Pus10 can also generate Ψ55, even in tRNAs that do not contain the Ψ54 synthase recognition sequence. This activity may be redundant with that of TruB.

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Section: Introductionmentioning

confidence: 54%

The human ortholog of archaeal Pus10 produces pseudouridine 54 in select tRNAs where its recognition sequence contains a modified residue

Deogharia

Mukhopadhyay

Joardar

et al. 2018

RNA

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“…where termination usually occurs. Examination of the nucleotide sequence in the anticodon loop of the normal bovine and rat liver Gln-tRNA (16,38) and of the amber suppressor tRNA (19) indicates that there is the possibility for interaction between two of the nucleotides of the mRNA with two of the nucleotides of the anticodon loop. In particular, the two bases immediately 3' to the amber codon in the message could potentially participate in hydrogen bonding with the two nucleotides 5' to the anticodon; Fig.…”

Section: Resultsmentioning

confidence: 99%

Retroviral gag gene amber codon suppression is caused by an intrinsic cis-acting component of the viral mRNA

Panganiban

1988

J Virol

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In some type C retroviruses, translation of the pol gene appears to require translational suppression of the proximal gag amber codon. To identify the region of the viral nucleic acid responsible for synthesis of the pol gene products, a 300-base-pair DNA fragment containing the stop codon from a type C murine virus (AK virus) was inserted into the Escherichia coli lacZ gene such that the translational reading frame was maintained.

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“…It is not clear whether any of these MuLV-related proviruses possess the capacity to replicate as a retrovirus. In a previous study (21), we found that a MuLV-related proviral DNA clone from BALB/c mice contained a distinctly different tRNA primer binding site that was not complementary to the 3'-terminal sequence of proline tRNA but showed a 17-of-18 match to that of a published glutamine tRNA structure (38). Here we present experimental evidence that this glutamine tRNA shows a perfect match to the primer binding site in three of the four other MuLV-related proviruses examined.…”

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confidence: 95%

“…Three points are evident from these sequence data. First, the 3'-terminal, 18-nucleotide sequence of a rat liver major isoacceptor glutamine tRNA (38), shown at the bottom of ... .…”

mentioning

confidence: 99%

“…Plus-stranded nucleotide sequences of the LTR uS, inverted repeat (IR), the tRNA primer binding site, and the immediate downstream variable (V) and conserved regions in five endogenous MuLV-related proviral DNA clones. The 3'-terminal 18 nucleotides of a major tRNAGIn isoacceptor (38) and tRNA"I°are shown at the bottom for comparison. With the pRFM-1 sequence as reference, identical bases of other sequences are shown as dots.…”

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confidence: 99%

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Nucleotide sequence analysis of endogenous murine leukemia virus-related proviral clones reveals primer-binding sites for glutamine tRNA

Nikbakht

Boone

et al. 1985

J Virol

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Nucleotide sequences of the region that corresponds to the site of tRNA primer binding for a functional retrovirus were determined in five murine leukemia virus-related sequence clones from mouse chromosomal DNA, which contain a unique 170 to 200-base-pair additional internal segment in the long terminal repeats. The 3'-terminal 18-nucleotide sequence of a major glutamine tRNA isoacceptor was found to match well with the putative primer binding site: 18 of 18 in three clones, 17 of 18 in one clone, and 16 of 18 in one clone. This implies that most of these endogenous proviral sequences of the mouse genome, if replicated as retroviruses, will be different from ecotropic murine leukemia viruses and most mammalian type C retroviruses in using glutamine tRNA, rather than proline tRNA, as a primer.

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The nucleotide sequence of a major glutamine tRNA from rat liver

Abstract: A major glutamine tRNA from rat l iver was purified. Post-label ing techniques showed its nucleotide sequence to be:

Cited by 17 publications

References 26 publications

The human ortholog of archaeal Pus10 produces pseudouridine 54 in select tRNAs where its recognition sequence contains a modified residue

The human ortholog of archaeal Pus10 produces pseudouridine 54 in select tRNAs where its recognition sequence contains a modified residue

Retroviral gag gene amber codon suppression is caused by an intrinsic cis-acting component of the viral mRNA

Nucleotide sequence analysis of endogenous murine leukemia virus-related proviral clones reveals primer-binding sites for glutamine tRNA

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