The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region

Burt, David W.; Mullins, L. J.; George, Helen; Smith, Gary Scott; Brooks, Jeanie I.; Pioli, David; Brammar, William J.

doi:10.1016/0378-1119(89)90143-1

Cited by 32 publications

(20 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Clearly, the identification of any sequences which specify position-independent, copy-number related expression is fundamental to future studies of renin gene control. In order to gain insight into the sequences controlling renin gene-expression, we have also (11,14). In order to distinguish the Ren-1D transgene and its transcript from the endogenous Ren-1C gene, we inserted a synthetic 30mer into exon 2.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of this transgene is also inducible with androgens (8). Sequence and heteroduplex analyses of cloned Ren-1C, Ren-lD and Ren-2 DNA demonstrate a. high degree of homology within the region of the structural gene (9,10,11). Differences between the flanking sequences of Ren-1C/D and Ren-2 can, however, be discerned and include a proviral intracisternal A particle located downstream of Ren-2 and a B2 repetitive element situated upstream of Ren-2 (12,13).…”

Section: Introductionmentioning

confidence: 99%

“…Such sequence variations represent putative cis-acting DNA elements which may be responsible for the different expression patterns observed between the Ren-1 and Ren-2 genes. In contrast, the flanking sequences of Ren-1C and Ren-1D are highly conserved and based upon this homology, the 98.4% homology of their coding sequences (11,14) and similar expression pattern in kidney and SMG, there is a general assumption that Ren-1 C and Ren-1 D display the same tissue-specificities and regulation. In this report, we have analysed renin gene expression in various extra-renal sites of oneand two-gene inbred mouse strains.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential extra-renal expression of the mouse renin genes

Miller¹,

Carter²,

Brooks³

et al. 1989

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

We have used RNase-protection analyses to study renin gene expression in one-and two-gene mouse strains. The RNase-protection assay is capable of discriminating between the transcripts from the different renin genes. In a two-gene strain containing Ren-1D and Ren-2, we demonstrate transcriptional activity from Ren-ID in kidney, submandibular gland (SMG), testes, liver, brain and heart. Ren-2 is clearly expressed in kidney, SMG and testes. Similar analyses of one gene strains (containing show expression in kidney, SMG, testes, brain and heart. We cannot detect renin mRNA in the liver of these mice.Ren-1C and Ren-1D thus display quite different tissue-specificities. In order to determine whether the different tissue-specificities of the highly homologous Ren-lC and Ren-1D genes are due to different trans-acting factors in the different mouse strains or to different cis-acting DNA elements inherent to the genes, we introduced a Ren-11D transgene (Ren-l*) into a background strain containing only the Ren-1C gene. The transgene exhibits the same tissue-specificity as the Ren-1D gene of two-gene strains suggesting the presence of different cis-acting DNA elements in Ren-1C and Ren-1D. INTRODUCTIONThe aspartyl protease renin catalyses the initial and rate-limiting step in the conversion of angiotensinogen to the vasoactive hormone angiotensin II and thus plays a key role in the regulation of blood pressure (1). The primary site of renin biosynthesis is the juxtaglomerular apparatus of the kidney.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential extra-renal expression of the mouse renin genes

Miller¹,

Carter²,

Brooks³

et al. 1989

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Two alleles from each of the 2 murine renin loci were compared throughout the sequenced region of the Ren-2 h allele ( Figure 2). Sequences for Ren-1 c (L78789) and Ren-1 d (M32352) were retrieved from GenBank, 14,15 whereas the sequence for Ren-2 h was derived from pGEMR2H-5, and the sequence for Ren-2 d was derived from pCATNOT R2-4.6. As noted above, the M3 element is present in both Ren-1 and Ren-2 loci, whereas the B2 element is found in the alleles of Ren-2 and not in the alleles of Ren-1.…”

Section: Abonia Et Al Nre Mediated Expression Of Murine Renin Genes 107mentioning

confidence: 99%

Evaluating a Model of an NRE Mediated Tissue-Specific Expression of Murine Renin Genes

et al. 2001

View full text Add to dashboard Cite

Abstract-We have used comparative sequence analysis to evaluate a putative silencer element that has been proposed to be involved in the differential tissue-expression of the murine renin genes: Ren-1 and Ren-2. In the mouse, these genes share a similar pattern of tissue-specific renin expression. One significant difference is seen in the submandibular gland (SMG) where renin expression from the Ren-2 locus is 100-fold greater than the expression from the Ren-1 locus. One model proposes that this differential expression arises from the interplay among a negative regulatory element and a cAMP responsive element, their respective binding factors, and the disruption of the negative regulatory element by an insertion (M2) that is found in Ren-2 but not in Ren-1. enin is an important regulatory enzyme involved in blood pressure maintenance and electrolyte homeostasis. It catalyzes the first reaction in the production of angiotensin II, a potent vasoactive hormone that is directly involved in both the circulating and tissue-specific renin-angiotensin systems. Many inbred strains of mice, such as DBA/2, which are derived from M. domesticus and M. musculus, possess an additional copy of the renin gene. This second locus arose as a tandem duplication to Ren-1. 1,2 Precisely the same duplication event is observed in the closely related subspecies M. hortulanus and M. spretus. 3 In these mouse species, the 2 renin genes, Ren-1 and Ren-2, share overlapping but nonidentical patterns of tissue-specific expression.The primary site of synthesis for circulating renin in all mouse strains is the kidney. Multiple alleles from both Ren-1hortulanus allele) and Ren-2 (Ren-2 d ϭ Renin-2 DBA/2J allele, Ren-2 h ϭ Renin-2 M. hortulanus allele) are expressed at approximately equal levels in the kidneys from 1-or 2-gene strains of mice. 3,4 In addition, a limited spectrum of extrarenal sites of tissue-renin production, including the adrenal gland, testes, ovaries, coagulating gland, liver, and submandibular gland (SMG), has also been identified. 5 The most dramatic difference in Ren-1 and Ren-2 expression has been noted in the SMG. In 2-gene strains of mice (DBA2/J and M. hortulanus), Ren-2 expression is Ͼ100-fold higher than expression of Ren-1 transcripts. Overall, the Ren-2 d and Ren-2 h alleles show a similar expression pattern as judged by the examination of a number of tissues, including SMG, kidney, testes, coagulating gland, and liver. Ren-1 d and Ren-1 h also share a similar pattern of renin expression, differing only in the increased expression of Ren-1 h in the liver relative to Ren-1 d . 5 Dzau and his colleagues 6 -9 have proposed that the similarities seen for Ren-2 and Ren-1 expression in the kidney, versus the differences seen in the SMG of DBA/2J, result from the complex interplay among a negative regulatory element (NRE), a cAMP responsive element (CRE), and their

show abstract

“…Ren-1 is located 3Ј to Ren-2, (see Figure 1) and is 97% homologous to Ren-2 at the nucleotide level. 17 The P1 clone, P1-1251 (Figure 1), was isolated and used to investigate the reproducibility of the targeting recombination reaction by introducing the ␤-Geo reporter construct 18 into the coding sequence of the Ren-1 gene. The reporter construct, ␤-Geo, is a fusion of the E. coli lacZ gene and the neomycin resistance gene.…”

mentioning

confidence: 99%

Manipulating large genomic clones via in vivo recombination in bacteria

Payne

Mullins

1999

J Hum Hypertens

View full text Add to dashboard Cite

Transgenesis is proving to be a powerful technique in studying the molecular genetics of hypertension. The ability to target specific mutations resulting in either loss of function, by gene deletion, the insertion of reporter sequences, or the subtle change of function by nucleotide replacement, can facilitate the understanding of gene function and its role in the manifestation of diseases. However an inherent problem associated with transgenic studies is the lack of consistent expression observed between independent lines of animals which have integrated the same transgene, a phenomenon known as 'position effect'. Small transgenes are almost invariably subject to position effect due to the absence of essential regulatory elements required to maintain an open chromatin structure. This phenomenon may be

show abstract

The nucleotide sequence of a mouse renin-encoding gene, Ren-1d, and its upstream region

Cited by 32 publications

References 44 publications

Differential extra-renal expression of the mouse renin genes

Differential extra-renal expression of the mouse renin genes

Evaluating a Model of an NRE Mediated Tissue-Specific Expression of Murine Renin Genes

Manipulating large genomic clones via in vivo recombination in bacteria

Contact Info

Product

Resources

About