The nucleotide sequence of a linear plasmid of Borrelia burgdorferi reveals similarities to those of circular plasmids of other prokaryotes

Barbour, Alan G.; Carter, C J; Bundoc, Virgilio; Hinnebusch, J

doi:10.1128/jb.178.22.6635-6639.1996

Cited by 40 publications

(40 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2. The terminal 26 bp of the B31 right chromosomal telomere sequence (C. Fraser, L. Lathigra and S. Casjens, unpublished) is more similar to previously analysed plasmid telomeres than it is to the other known chromosomal telomeres, and it has an additional 35 bp of homology to the previously characterized lp16 left telomere (Hinnebusch et al, 1990;Barbour et al, 1996) immediately internal to the 26 bp terminal inverted repeat.…”

Section: Lyme Agent Chromosomal Telomeresmentioning

confidence: 70%

Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres

Casjens

Murphy

DeLange

et al. 1997

Molecular Microbiology

109

View full text Add to dashboard Cite

SummaryBacteria of the spirochaete genus Borrelia have linear chromosomes about 950 kbp in size. We report here that these linear chromosomes have covalently closed hairpin structures at their termini that are similar but not identical to those reported for linear plasmids carried by these organisms. Nucleotide sequence analysis of the chromosomal telomeric regions indicates that unique, apparently functional genes lie within a few hundred bp of each of the telomeres, and that there is an imperfect 26 bp inverted repeat at the two telomeres. In addition, we characterize a major chromosomal length polymorphism within the right telomeric regions of various Borrelia isolates, and show that sequences similar to those near the right telomere are often found on linear plasmids in B. burgdorferi (sensu stricto) isolates from nature. Sequences similar to a number of other regions of the chromosome, including those near the left telomere, were not found on B. burgdorferi plasmids. These observations suggest that there has been historical exchange of genetic information between the linear plasmids and the right end of the linear chromosome.

show abstract

Section: Lyme Agent Chromosomal Telomeresmentioning

confidence: 70%

Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres

Casjens

Murphy

DeLange

et al. 1997

Molecular Microbiology

109

View full text Add to dashboard Cite

show abstract

“…However, in their analysis of the B. burgdorferi plasmid sequences, Casjens et al (2000) noted that clusters of two to four genes, composed of members of paralogous families 32, 49, 50, 57, and 62, occur on all 21 linear and circular plasmids. The family 32 genes are similar to the known partition protein gene parA (Barbour et al 1996;Zuckert and Meyer 1996) and occur on all B. burgdorferi replicons except the small lp5 and cp9 (Fraser et al 1997;Casjens et al 2000). The predicted proteins encoded by the plasmid-borne family 32 paralogs also have a 51% to 62% similarity to Soj.…”

Section: Genome Research 1597mentioning

confidence: 92%

“…Barbour et al (1996) and Zuckert and Meyer (1996) noted the soj/parA-like genes on lp17 and cp32 of B. burgdorferi, and Stevenson et al (1998) suggested that the heterogeneity of these genes among a family of closely related cp32 plasmids could account for their mutual compatibility. This may be more generally true, because each B. burgdorferi replicon appears to contain a unique origin sequence and different alleles of a soj/ parA homolog and other paralogous gene families that may be involved in partitioning.…”

Section: B Burgdorferi Plasmid Replication Originsmentioning

confidence: 99%

Analyzing DNA Strand Compositional Asymmetry to Identify Candidate Replication Origins of Borrelia burgdorferi Linear and Circular Plasmids

Picardeau¹,

Lobry²,

Hinnebusch³

2000

Genome Res.

View full text Add to dashboard Cite

The Lyme disease agent Borrelia burgdorferi has a genome composed of a linear chromosome and a series of linear and circular plasmids. We previously mapped the oriC of the linear chromosome to the center of the molecule, where a pronounced switch in CG skew occurs. In this study, we analyzed B. burgdorferi plasmid sequences for AT and CG skew in an effort to similarly identify plasmid replication origins. Cumulative skew diagrams of the plasmids suggested that they, like the linear chromosome, replicate bidirectionally from an internal origin. The B. burgdorferi linear chromosome contains homologs to partitioning protein genes soj and spoOJ, which are closely linked to oriC at the minimum cumulative skew point of the 1-Mb molecule. A soj/parA homolog also maps to cumulative skew minima of the B. burgdorferi linear and circular plasmids, further suggesting that these regions contain the replication origin. The heterogeneity in these genes and in the nucleotide sequences of the putative origin regions could account for the mutual compatibility of the multiple DNA elements in B. burgdorferi.

show abstract

“…Second, loss of plasmids during continuous in vitro cultivation results in diminished infectivity (35,42,61,63). B. burgdorferi plasmids are also of interest because they contain multiple paralogous genes which appear to have undergone extensive recombination during their evolution (2,5,8,10,15,20,39,45,49,64).…”

mentioning

confidence: 99%

Molecular and Evolutionary Characterization of the cp32/18 Family of Supercoiled Plasmids in Borrelia burgdorferi 297

et al. 2000

View full text Add to dashboard Cite

In this study, we characterized seven members of the cp32/18 family of supercoiled plasmids in Borrelia burgdorferi 297. Complete sequence analysis of a 21-kb plasmid (cp18-2) confirmed that the strain 297 plasmids are similar in overall content and organization to their B31 counterparts. Of the 31 open reading frames (ORFs) in cp18-2, only three showed sequence relatedness to proteins with known functions, and only one, a ParA/SopA ortholog, was related to nonborrelial polypeptides. Besides the lipoproteins, none of the ORFs appeared likely to encode a surface-exposed protein. Comparison with the B31 genomic sequence indicated that paralogs for most of the ORFs in cp18-2 can be identified on other genetic elements. cp18-2 was found to lack a 9-to 10-kb fragment present in the 32-kb homologs which, by extrapolation from the B31 cp32 sequences, contains at least 15 genes presumed to be unnecessary for plasmid maintenance. Sequence analysis of the lipoprotein-encoding variable loci provided evidence that recombinatorial processes within these regions may result in the acquisition of exogenous DNA. Pairwise analysis with random shuffling revealed that the multiple lipoproteins (Mlp; formerly designated 2.9 LPs) fall into two distinct homology groups which appear to have arisen by gene fusion events similar to those recently proposed to have generated the three OspE, OspF, and Elp lipoprotein families

show abstract

The nucleotide sequence of a linear plasmid of Borrelia burgdorferi reveals similarities to those of circular plasmids of other prokaryotes

Cited by 40 publications

References 40 publications

Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres

Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres

Analyzing DNA Strand Compositional Asymmetry to Identify Candidate Replication Origins of Borrelia burgdorferi Linear and Circular Plasmids

Molecular and Evolutionary Characterization of the cp32/18 Family of Supercoiled Plasmids in Borrelia burgdorferi 297

Contact Info

Product

Resources

About