The nucleotide sequence of a glutamate tRNA from rat liver

Abstract

A glutamate tRNA from rat liver was purified. By means of post-labeling techniques, its nucleotide sequence was shown to be: pU-C-C-C-A-C-A-U-m1G-G-U-C-psi-A-G-C- G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-psi-U-mcm5s2U-U-C-A-C-C-C-A-G-G-C-G- G-C-m5C-m5C-G-G-G-Tm-psi-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH. The sequence is remarkably similar to that of tRNA4Glu from Drosophila melanogaster. Only 10 out of 75 nucleotides in the two tRNAs are different.

Genome-wide analysis of N1-methyl-adenosine modification in human tRNAs

“…The identity of m 1 A58 hypomodified tRNA species is similar in five human cell lines examined, suggesting that human cells share a common pattern of m 1 A58 hypomodification. As positive controls for our genomic approach, we identified that the initiator-tRNA Met is fully modified, and the tRNA Asp and tRNA Glu species are hypomodified in all five cell lines, in agreement with the literature results (Kuchino et al 1981;Chan et al 1982). It is an open question as to whether m 1 A58 modification is essential for the maturation of the majority of human tRNAs or whether fully modified tRNAs are simply more efficient substrates for the m 1 A58 methyltransferase.…”

“…These isoacceptors have very similar sequences and cannot be separately detected in our array method (Dittmar et al 2006). Three tRNAs with detectable signals are tRNA Asp and two isoacceptor families of tRNA Glu ; these tRNAs have been shown previously to be undermodified at A58 in rat cells (Kuchino et al 1981;Chan et al 1982), and therefore likely to be hypomodified in human cells as well. On the other hand, no signal was detected for the initiator-tRNA Met ( Fig.…”

“…Among the over 30 tRNAs sequenced, only two, rat tRNA Asp and tRNA Glu , have been reported to be unmodified at position 58 (Kuchino et al 1981;Chan et al 1982). The human homolog of the yeast tRNA m 1 A methyltransferase has been identified and it has been shown to have the same two subunit composition as the methyltransferase from yeast (Ozanick et al 2005).…”

tRNA base methylation identification and quantification via high-throughput sequencing

“…3C), the only other tRNA in the low group is GluCTC. Both Asp and Glu tRNA results are consistent with literature (Kuchino et al 1981;Chan et al 1982) that these tRNAs are hypomodified at m 1 A58. We also validate the low m 1 A58 modification in tRNA Asp by primer extension (Fig.…”

“…These tRNAs may have different modification patterns depending on sequence, anticodon stem-loop context, and other factors. Although it is commonly assumed that many modification sites in tRNA are fully modified, previous literature provides scattered evidence that certain modification sites in specific tRNAs can be fractionally modified (Kuchino et al 1981;Chan et al 1982;Saikia et al 2010;Vinayak and Pathak 2010;Hauenschild et al 2015), suggesting that dynamic differences in tRNA modification may be a potentially useful parameter for biological regulation.…”