The nucleotide sequence of 3-isopropylmalate dehydrogenase gene fromBacillus subtilis

Regulatory Region of Expression ofThiobacillus ferrooxidans leuBGene inEscherichia coli

“…In bacteria, leucine is biosynthesized by the actions of four enzymes, 2-isopropylmalate synthase encoded by leuA, isopropylmalate isomerase encoded by leuC and feuD, 3-isopropylmalate dehydrogenase encoded by leuB, and aminotransferase. 3-lsopropylmalate dehydrogenase [EC 1.1.1.85J, a key enzyme in leucine biosynthesis, catalyzes the oxidative decarboxylation of the substrate 3-isopropylmalate simultaneously with dehydrogenation.…”

“…The enzyme has been found in a wide variety of bacteria and the amino acid sequence of the enzyme from various microorganisms including yeast shows high similarity. [1][2][3][4][5][6][7][8][9][10] In the case of Escherichia coli. 11) Salmonel/a typhimurium, 12) and Lactococcus lactis,5) the biosynthesis of leucine is controlled by a cluster of four structural genes and an adjacent operator region with the gene order ~lcuA leuB ZeuC leuD-.…”

Cloning of genes for proline and leucine biosynthesis from Brucella abortus by functional complementation in Escherichia coli

“…In most cases, only the cloning was noted, but a few of the genes have been studied in some detail. DNA sequences have been obtained for proA from Serratia marcescens (Omori et al, 1991) and E. coli (Deutch et al, 1984), and for leuB from several organisms (Sekiguchi et al, 1986(Sekiguchi et al, , 1987Imai et al 1987;Kirino & Oshima, 1991). We have isolated B. abortus DNA clones that restore enzyme activity for y-glutamylphosphate reductase and p-isopropylmalate dehydrogenase in E. coli strains deficient in these enzymes.…”

Domain Motion in 3-isopropylmalate dehydrogenase: A Strategy to Enhance its Thermal Stability

“…The genes of the enzyme have been cloned and sequenced from various organisms with a variety of growth temperatures including psychrotrophic (cold-loving), mesotrophic, and thermotrophic organisms (1)(2)(3)(4)(5)(6)(7)(8).…”