The nuclear factor kappa B (NF-κB) activation is required for phagocytosis of staphylococcus aureus by RAW 264.7 cells

Zhu, Faming; Yue, Wan‐Fu; Wang, Yongxia

doi:10.1016/j.yexcr.2014.04.018

Cited by 38 publications

(29 citation statements)

References 55 publications

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“…Mammalian target of rapamycin (mTOR), which is an atypical serine/threonine protein kinase, serves as the phosphoinositide 3‐kinase (PI3K)‐related kinase family and forms two kinds of different complexes named mTORC1 and mTORC2 (Okamoto et al, ). Nuclear factor‐kappa B (NF‐κB), a ubiquitous and momentous transcription factor with various stimulants including growth factors and cytokines, which can control the expressions of a variety of genes referring to immune responses (Miller et al, ; Zhu, Yue, & Wang, ). Ahmad et al () also showed that NF‐κB pathway could function as a key central pathway interacting with various downstream and upstream signaling pathways, including the mTOR pathway.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Effects of mTOR/NF‐κB signaling pathway and high thoracic epidural anesthesia on myocardial ischemia‐reperfusion injury via autophagy in rats

Huang

Wen

Lin

et al. 2018

Journal Cellular Physiology

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We investigated the role of mammalian target of rapamycin/nuclear factor-kappa B (mTOR/NF-κB) signaling pathway in high thoracic epidural anesthesia (HTEA) against myocardial ischemia-reperfusion (I/R) injury in rats. The rat model of myocardial I/R injury was established. Ninety rats were divided into the normal, sham, I/R, eHTEA, the PDTC, and HTEA + PDTC groups. ELISA was applied to detect cardiac function indexes. HE staining was conducted to observe histopathological changes of myocardial tissues, and TTC staining was performed to detect the myocardial infarction size. TUNEL staining was adopted to detect the cell apoptosis rate. The mRNA and protein levels of mTOR, NF-κB, Fasl, Bcl-2 and Bax, and LC3-I, LC3-II, BNIP3, and Atg5 were detected by RT-qPCR and Western blotting, respectively. The findings indicated that compared with the normal and sham groups, the I/R, PDTC, and HTEA groups showed the larger myocardial infarction size and increased cell apoptosis rate, while the results in the HTEA + PDTC group were opposite. Compared with the normal and sham groups, the I/R group showed reduced mRNA and protein levels of Bcl-2, LC3, BNIP3, and Atg5, and elevated mRNA and protein levels of mTOR, p50, p65, Bax, and Fasl, while the HTEA + PDTC group revealed the opposite results, and the PDTC and HTEA group revealed the increased mRNA and protein levels of Bcl-2, LC3, BNIP3, Atg5, mTOR, p50, p65, Bax, and Fasl. These results prove that the inhibition of mTOR/NF-κB signaling pathway potentiates HTEA against myocardial IR injury by autophagy and apoptosis in rats.

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Section: Introductionmentioning

confidence: 99%

Retracted: Effects of mTOR/NF‐κB signaling pathway and high thoracic epidural anesthesia on myocardial ischemia‐reperfusion injury via autophagy in rats

Huang

Wen

Lin

et al. 2018

Journal Cellular Physiology

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“…TNFα, an activator of NFκB, induced Tudor-SN expression [ 14 ]. NFκB is a ubiquitous transcription factor involved in cell proliferation and differentiation [ 42 , 43 ]. Our previous study found that Met and E triggered NFκB1 phosphorylation and enhanced the binding of p-NFκB1 to the Stat5 gene promoter (data not shown), and in this study we also validated the interaction of p-NFκB1 and Tudor-SN gene promoter.…”

Section: Resultsmentioning

confidence: 99%

Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells

Wei

et al. 2015

IJMS

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Tudor staphylococcal nuclease (Tudor-SN) is a highly conserved and ubiquitously expressed multifunctional protein, related to multiple and diverse cell type- and species-specific cellular processes. Studies have shown that Tudor-SN is mainly expressed in secretory cells, however knowledge of its role is limited. In our previous work, we found that the protein level of Tudor-SN was upregulated in the nucleus of bovine mammary epithelial cells (BMEC). In this study, we assessed the role of Tudor-SN in milk synthesis and cell proliferation of BMEC. We exploited gene overexpression and silencing methods, and found that Tudor-SN positively regulates milk synthesis and proliferation via Stat5a activation. Both amino acids (methionine) and estrogen triggered NFκB1 to bind to the gene promoters of Tudor-SN and Stat5a, and this enhanced the protein level and nuclear localization of Tudor-SN and p-Stat5a. Taken together, these results suggest the key role of Tudor-SN in the transcriptional regulation of milk synthesis and proliferation of BMEC under the stimulation of amino acids and hormones.

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“…Nuclear factor kappa B (NF-kB) may regulate phagocyte activation via the induction of cytokine production. 13 Khan et al 14 studied the time course of the effects of 10 and 50 nm diameter naked GNPs on the expression of proinflammatory cytokines, including interleukin (IL)-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α), in the liver and kidneys of rats. In the liver, the GNPs of both sizes (10 and 50 nm) significantly increased cytokine gene expression on Day 1, which subsided by Day 5; however, the 50 nm GNPs produced a more severe inflammatory response compared to the 10 nm GNPs.…”

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confidence: 99%

Effect of inhibitors of endocytosis and NF-kB signal pathway on folate-conjugated nanoparticle endocytosis by rat Kupffer cells

Tang¹,

Chen²,

Jia³

et al. 2017

IJN

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The regular accumulation of nanoparticles in the liver makes them hepatotoxic and decreases the circulation time, thus reducing their therapeutic effect. Resolving this problem will be significant in improving bioavailability and reducing side effects. In this study, we reduced the phagocytosis of epirubicin (EPI)-loaded folic acid-conjugated pullulan acetate (FPA/EPI) nanoparticles by Kupffer cells (KCs) through internalization and nuclear factor kappa B (NF-kB) signal pathway inhibitors, thus allowing development of FPA/EPI nanoparticles as a nanodrug delivery system (NDDS) based on our previous study. FPA/EPI nanoparticles were prepared by the dialysis method. Rat KCs were preincubated with the following individual or compound inhibitors: chlorpromazine (CPZ), nystatin (NY), colchicine (Col), amiloride (AMR), and pyrrolidine dithiocarbamate (PDTC). Dose- and time-dependent cellular uptake effects of inhibitors on FPA/EPI nanoparticles were determined through fluorometry. The cytokine levels of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and IL-6 were tested in culture supernatants by bead-based multiplex flow cytometry. The uptake study demonstrated that inhibitors had an obvious inhibitory effect ( P <0.05 or P <0.01), with NY, AMR and Col all showing time-dependent inhibitory effects. PDTC + NY had the strongest inhibitory effect, with an uptake rate of 14.62%. The levels of the three proinflammatory cytokines were changed significantly by the compound inhibitors. TNF-α was significantly inhibited ( P <0.05 or P <0.01), but IL-1β and IL-6 showed smaller decreases. These results suggested that clathrin- and caveolae-mediated endocytosis were the main routes via which nanoparticles entered KCs and that the NF-kB signal pathway was very important too. In summary, multiple mechanisms, including clathrin- and caveolae-mediated endocytosis, contribute to cytokine production in macrophages following exposure to folic acid-conjugated pullulan acetate nanoparticles. Thus, the endocytosis inhibition strategy has great potential for improving therapy and reducing toxicity of an NDDS in the treatment of cancer.

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The nuclear factor kappa B (NF-κB) activation is required for phagocytosis of staphylococcus aureus by RAW 264.7 cells

Cited by 38 publications

References 55 publications

Retracted: Effects of mTOR/NF‐κB signaling pathway and high thoracic epidural anesthesia on myocardial ischemia‐reperfusion injury via autophagy in rats

Retracted: Effects of mTOR/NF‐κB signaling pathway and high thoracic epidural anesthesia on myocardial ischemia‐reperfusion injury via autophagy in rats

Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells

Effect of inhibitors of endocytosis and NF-kB signal pathway on folate-conjugated nanoparticle endocytosis by rat Kupffer cells

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