The Nuclear Envelope – Structure and Protein Interactions

Graumann, Katja; Evans, David

doi:10.1002/9781119312994.apr0498

Cited by 6 publications

(4 citation statements)

References 137 publications

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“…The nucleus itself is a dynamic structure, organized largely by its enclosing membrane system - the nuclear envelope (NE). The NE is a double-membraned structure composed of an outer nuclear membrane (ONM) and an inner nuclear membrane (INM) connected at nuclear pores (reviewed by Hetzer, 2010 ; Graumann and Evans, 2017 ). The functional properties of the NE are mediated by protein complexes linked to myriad cellular and nuclear processes, including cell division and gene expression ( Kim et al, 2015 ; Meier et al, 2017 ; De Magistris and Antonin, 2018 ; Pradillo et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability

et al. 2021

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In eukaryotes, the nuclear envelope (NE) encloses chromatin and separates it from the rest of the cell. The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex physically bridges across the NE, linking nuclear and cytoplasmic components. In plants, these LINC complexes are beginning to be ascribed roles in cellular and nuclear functions, including chromatin organization, regulation of nuclei shape and movement, and cell division. Homologs of core LINC components, KASH and SUN proteins, have previously been identified in maize. Here, we characterized the presumed LINC-associated maize nucleoskeletal proteins NCH1 and NCH2, homologous to members of the plant NMCP/CRWN family, and MKAKU41, homologous to AtKAKU4. All three proteins localized to the nuclear periphery when transiently and heterologously expressed as fluorescent protein fusions in Nicotiana benthamiana. Overexpression of MKAKU41 caused dramatic changes in the organization of the nuclear periphery, including nuclear invaginations that stained positive for non-nucleoplasmic markers of the inner and outer NE membranes, and the ER. The severity of these invaginations was altered by changes in LINC connections and the actin cytoskeleton. In maize, MKAKU41 appeared to share genetic functions with other LINC components, including control of nuclei shape, stomatal complex development, and pollen viability. Overall, our data show that NCH1, NCH2, and MKAKU41 have characteristic properties of LINC-associated plant nucleoskeletal proteins, including interactions with NE components suggestive of functions at the nuclear periphery that impact the overall nuclear architecture.

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Section: Introductionmentioning

confidence: 99%

Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability

et al. 2021

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“…The NE has many functions beyond the one of a simple barrier (Graumann and Evans, 2013;Méjat and Misteli, 2010). It regulates exchanges between the nucleus and the cytoplasm via the nuclear pore complex (Adams and Wente, 2013;Tamura et al, 2010), organises telomeres, connects the centromere to the centrosome during cell division, and bridges nucleus and cytoskeleton via the linker of nucleoskeleton and cytoskeleton (LINC) complex (Crisp et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants

Poulet

Duc

Voisin

et al. 2017

Journal of Cell Science

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The linker of nucleoskeleton and cytoskeleton (LINC) complex is an evolutionarily well-conserved protein bridge connecting the cytoplasmic and nuclear compartments across the nuclear membrane. While recent data support its function in nuclear morphology and meiosis, its involvement in chromatin organisation has not been studied in plants. Here, 3D imaging methods have been used to investigate nuclear morphology and chromatin organisation in interphase nuclei of the model plant Arabidopsis thaliana in which heterochromatin clusters in conspicuous chromatin domains called chromocentres. Chromocentres form a repressive chromatin environment contributing to transcriptional silencing of repeated sequences, a general mechanism needed for genome stability. Quantitative measurements of the 3D position of chromocentres indicate their close proximity to the nuclear periphery but that their position varies with nuclear volume and can be altered in specific mutants affecting the LINC complex. Finally, we propose that the plant LINC complex contributes to proper heterochromatin organisation and positioning at the nuclear periphery, since its alteration is associated with the release of transcriptional silencing as well as decompaction of heterochromatic sequences.

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“…Nuclear pore complexes (NPC), also known as SUN-KASH (INM Sad1/UNC-84 and Klarsicht/ANC-1/Syne-1 homology) nuclear envelope (NE) bridges are responsible for connecting the nucleoplasm and cytoplasm in eukaryotes (Graumann and Evans 2017;Yang et al 2017;Zhou et al 2012Zhou et al , 2015b. Nuclear transport of immune receptors, signal transducers and transcription factors is an essential regulatory mechanism for immune activation (Gu et al 2016).…”

Section: Immunity and Development Battle For Nucleocytoplasmic Transp...mentioning

confidence: 99%

A necessary considering factor for breeding: growth-defense tradeoff in plants

et al. 2023

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Crop diseases cause enormous yield losses and threaten global food security. Deployment of resistant cultivars can effectively control the disease and to minimize crop losses. However, high level of genetic immunity to disease was often accompanied by an undesired reduction in crop growth and yield. Recently, literatures have been rapidly emerged in understanding the mechanism of disease resistance and development genes in crop plants. To determine how and why the costs and the likely benefit of resistance genes caused in crop varieties, we re-summarized the present knowledge about the crosstalk between plant development and disease resistance caused by those genes that function as plasma membrane residents, MAPK cassette, nuclear envelope (NE) channels components and pleiotropic regulators. Considering the growth-defense tradeoffs on the basis of current advances, finally, we try to understand and suggest that a reasonable balancing strategies based on the interplay between immunity with growth should be considered to enhance immunity capacity without yield penalty in future crop breeding.

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The Nuclear Envelope – Structure and Protein Interactions

Cited by 6 publications

References 137 publications

Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability

Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability

The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants

A necessary considering factor for breeding: growth-defense tradeoff in plants

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