The NS3 protein of <i>Rice hoja blanca tenuivirus</i> suppresses RNA silencing in plant and insect hosts by efficiently binding both siRNAs and miRNAs

Hemmes, Hans; Lakatos, L; Goldbach, Rob; Burgyán, József; Prins, Marcel

doi:10.1261/rna.444007

Cited by 98 publications

(87 citation statements)

References 57 publications

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“…(Csorba et al 2007;Harries et al 2008;Hemmes et al 2007;Kubota et al 2003;Lakatos et al 2006;Merai et al 2005Merai et al , 2006Silhavy et al 2002). Amongst these, probably the best known is the tombusviral p19 protein .…”

Section: Vsirna Sequestrationmentioning

confidence: 99%

Antiviral Silencing and Suppression of Gene Silencing in Plants

Csorba

Burgyán

2016

Current Research Topics in Plant Virology

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RNA silencing is an evolutionary conserved sequence-specific gene inactivation mechanism that contributes to the control of development, maintains heterochromatin, acts in stress responses, DNA repair and defends against invading nucleic acids like transposons and viruses. In plants RNA silencing functions as one of the main immune systems. RNA silencing process involves the small RNAs and trans factor components like Dicers, Argonautes and RNA-dependent RNA polymerases. To deal with host antiviral silencing responses viruses evolved mechanisms to avoid or counteract this, most notably through expression of viral suppressors of RNA silencing. Due to the overlap between endogenous and antiviral silencing pathways while blocking antiviral pathways viruses also impact endogenous silencing processes. Here we provide an overview of antiviral silencing pathway, host factors implicated in it and the crosstalk between antiviral and endogenous branches of silencing. We summarize the current status of knowledge about the viral counter-defense strategies acting at various steps during virus infection in plants with the focus on representative, well studied silencing suppressor proteins. Finally we discuss future challenges of the antiviral silencing and counter-defense research field.

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Section: Vsirna Sequestrationmentioning

confidence: 99%

Antiviral Silencing and Suppression of Gene Silencing in Plants

Csorba

Burgyán

2016

Current Research Topics in Plant Virology

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“…In addition to interfere with IFN signaling pathway, viruses in general (Hemmes et al, 2007) and HCV in particular are able to interfere with RNAi-dependent antiviral effect. Using cell lines that express constitutively green fluorescent protein (GFP) and in which HCV protein expression is inducible, Ji et al demonstrated that silencing of GFP gene by siRNA was inhibited in the presence of the full length HCV polyprotein ; HCV E2 protein was shown to be responsible for this inhibition (Ji et al, 2008).…”

Section: Interferon Signaling Pathway and Viral Resistancementioning

confidence: 99%

Cellular models for the screening and development of anti-hepatitis C virus agents

Gondeau

Pichard-Garcia

Maurel

2009

Pharmacology & Therapeutics

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Investigations on the biology of hepatitis C virus (HCV) have been hampered by the lack of small animal models. Efforts have therefore been directed to designing practical and robust cellular models of human origin able to support HCV replication and production in a reproducible, reliable and consistent manner. Many different models based on different forms of virions and hepatoma or other cell types have been described including virus-like particles, pseudotyped particles, subgenomic and full length replicons, virion productive replicons, immortalised hepatocytes, fetal and adult primary human hepatocytes. This review focuses on these different cellular models, their advantages and disadvantages at the biological and experimental levels, and their respective use for evaluating the effect of antiviral molecules on different steps of HCV biology including virus entry, replication, particles generation and excretion, as well as on the modulation by the virus of the host cell response to infection.

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“…RHBV is transmitted by insect vector and observed to be having life cycles in both plants and insects. RHBV nonstructural protein NS3, encoded by RNA3, has been reported to be an RNA silencing suppressor that functions to evade the antiviral silencing mechanism in plants, insect cells and mammalian cells (Bucher et al 2003;Hemmes et al 2007;Schnettler et al 2008Schnettler et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus

Xia

Tan²,

Teh³

et al. 2011

RNA

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Rice Hoja Blanca Tenuivirus (RHBV), a negative strand RNA virus, has been identified to infect rice and is widely transmitted by the insect vector. NS3 protein encoded by RHBV RNA3 was reported to be a potent RNAi suppressor to counterdefense RNA silencing in plants, insect cells, and mammalian cells. Here, we report the crystal structure of the N-terminal domain of RHBV NS3 (residues 21-114) at 2.0 Å . RHBV NS3 N-terminal domain forms a dimer by two pairs of a-helices in an anti-parallel mode, with one surface harboring a shallow groove at the dimension of 20 Å 3 30 Å for putative dsRNA binding. In vitro RNA binding assay and RNA silencing suppression assay have demonstrated that the structural conserved residues located along this shallow groove, such as Arg50, His51, Lys77, and His85, participate in dsRNA binding and RNA silencing suppression. Our results provide the initial structural implications in understanding the RNAi suppression mechanism by RHBV NS3.

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The NS3 protein of Rice hoja blanca tenuivirus suppresses RNA silencing in plant and insect hosts by efficiently binding both siRNAs and miRNAs

Cited by 98 publications

References 57 publications

Antiviral Silencing and Suppression of Gene Silencing in Plants

Antiviral Silencing and Suppression of Gene Silencing in Plants

Cellular models for the screening and development of anti-hepatitis C virus agents

Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus

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