The NPR1 family of transcription cofactors in papaya: insights into its structure, phylogeny and expression

Peraza-Echeverría, Santy; Santamaría, Jorge M.; Fuentes, Gabriela RamÃ­rez; Menéndez-Cerón, Mariana de los Ángeles; Vallejo-Reyna, Miguel Ángel; Herrera-Valencia, Virginia Aurora

doi:10.1007/s13258-011-0218-7

Cited by 18 publications

(20 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phylogenetic analysis reveals that all five PaNPR1-like proteins group with other known NPR1-like sequences, clustering into three distinct clades ( Peraza-Echeverria et al, 2012 ). PaNPR1 and PaNPR2 fall within the same clade as AtNPR1, which is a known positive regulator of SAR ( Cao et al, 1997 , 1998 ).…”

Section: Discussionmentioning

confidence: 99%

“…Multiple NPR1-like proteins seem to be present in most, if not all, plant species. Phylogenetic analysis of this family suggests the existence of three functionally distinct clades ( Hepworth et al, 2005 ; Zhang et al, 2006 ; Peraza-Echeverria et al, 2012 ). Members of the first clade, AtNPR1 and AtNPR2, are mostly associated with positive SAR regulation ( Cao et al, 1997 , 1998 ).…”

Section: Introductionmentioning

confidence: 99%

“…The third clade, AtBOP1, and AtBOP2, is associated with the development of lateral organs ( Hepworth et al, 2005 ). Phylogenetic analysis has since included NPR1-like proteins from multiple plant species ( Le Henanff et al, 2009 ; Peraza-Echeverria et al, 2012 ; Shao et al, 2013 ), and although phylogenetic analysis alone is insufficient for functional annotation it may provide a basis for understanding functional variation ( Liu et al, 2005 ; Zhang et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)

et al. 2015

View full text Add to dashboard Cite

The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1) forms an integral part of the salicylic acid (SA) pathway in plants and is involved in cross-talk between the SA and jasmonic acid/ethylene (JA/ET) pathways. Therefore, NPR1 is essential to the effective response of plants to pathogens. Avocado (Persea americana) is a commercially important crop worldwide. Significant losses in production result from Phytophthora root rot, caused by the hemibiotroph, Phytophthora cinnamomi. This oomycete infects the feeder roots of avocado trees leading to an overall decline in health and eventual death. The interaction between avocado and P. cinnamomi is poorly understood and as such limited control strategies exist. Thus uncovering the role of NPR1 in avocado could provide novel insights into the avocado – P. cinnamomi interaction. A total of five NPR1-like sequences were identified. These sequences were annotated using FGENESH and a maximum-likelihood tree was constructed using 34 NPR1-like protein sequences from other plant species. The conserved protein domains and functional motifs of these sequences were predicted. Reverse transcription quantitative PCR was used to analyze the expression of the five NPR1-like sequences in the roots of avocado after treatment with salicylic and jasmonic acid, P. cinnamomi infection, across different tissues and in P. cinnamomi infected tolerant and susceptible rootstocks. Of the five NPR1-like sequences three have strong support for a defensive role while two are most likely involved in development. Significant differences in the expression profiles of these five NPR1-like genes were observed, assisting in functional classification. Understanding the interaction of avocado and P. cinnamomi is essential to developing new control strategies. This work enables further classification of these genes by means of functional annotation and is a crucial step in understanding the role of NPR1 during P. cinnamomi infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)

et al. 2015

View full text Add to dashboard Cite

show abstract

“…For example, in avocado ( Persea a mericana ) three out of five AtNPR1 -like genes showed a possible role in plant defense 74 . In papaya ( Carica papaya ), four homologous genes of AtNPR1 were identified and their expression pattern in different tissues was evaluated 75 .…”

Section: Use Of Atnpr1 and Its Orthologs For Crop mentioning

confidence: 99%

NPR1 as a transgenic crop protection strategy in horticultural species

et al. 2018

View full text Add to dashboard Cite

The NPR1 (NONEXPRESSOR OF PATHOGENESIS RELATED GENES1) gene has a central role in the long-lasting, broad-spectrum defense response known as systemic acquired resistance (SAR). When overexpressed in a transgenic context in Arabidopsis thaliana, this gene enhances resistance to a number of biotic and abiotic stresses. Its position as a key regulator of defense across diverse plant species makes NPR1 a strong candidate gene for genetic engineering disease and stress tolerance into other crops. High-value horticultural crops face many new challenges from pests and pathogens, and their emergence exceeds the pace of traditional breeding, making the application of NPR1-based strategies potentially useful in fruit and vegetable crops. However, plants overexpressing NPR1 occasionally present detrimental morphological traits that make its application less attractive. The practical utility of NPR-based approaches will be a balance of resistance gains versus other losses. In this review, we summarize the progress on the understanding of NPR1-centered applications in horticultural and other crop plants. We also discuss the effect of the ectopic expression of the A. thaliana NPR1 gene and its orthologs in crop plants and outline the future challenges of using NPR1 in agricultural applications.

show abstract

“…Recently, the complete NBS-LRR family of disease resistance genes of papaya was characterized in silico [12]. We have also taken advantage of the papaya genome sequence by characterizing the structure and expression of the full set of NPR1 and TGA transcription factors in this crop [13,14]. In the case of the papaya AP2/ ERF family, 146 of these genes were predicted by Ming et al [11], however, these genes were not further characterized.…”

Section: Introductionmentioning

confidence: 99%

Identification of novel ERF transcription factor genes in papaya and analysis of their expression in different tissues and in response to the plant defense inducer benzothiadiazole (BTH)

Vallejo-Reyna

Santamaría

Rodríguez-Zapata

et al. 2015

Physiological and Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

The NPR1 family of transcription cofactors in papaya: insights into its structure, phylogeny and expression

Cited by 18 publications

References 75 publications

Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)

Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.)

NPR1 as a transgenic crop protection strategy in horticultural species

Identification of novel ERF transcription factor genes in papaya and analysis of their expression in different tissues and in response to the plant defense inducer benzothiadiazole (BTH)

Contact Info

Product

Resources

About