The novel six LIM and one PET domain-containing protein Lmpt is involved in the immune response through activation of the NF-κB signalling pathway in the crustacean, Macrobrachium nipponense

Shrimp immune response: A transcriptomic perspective

“…Using BLAST 15 and BLAST2GO, 16,17 they classified 11,153 unigenes into 25 categories of cluster of orthologous groups, 8171 unigenes were assigned to 51 functional groups of GO and 18,154 unigenes were divided into 220 KEGG pathways. Since then, a series of important processes related to the immune system in penaeid shrimps have been identified, among which we can mention the role of the haemocytes, [18][19][20][21][22][23][24][25] autophagy, 26,27 phagocytosis, 28 antimicrobial peptides (AMPs) including anti-lipopolysaccharide factors (ALF4) and crustins (CRU1, CRU2), 29 and the prophenoloxidase (PPO) system, [30][31][32][33] as well as important enzymes such as transglutaminase (TGM) and lysozyme (LYS), 30,[33][34][35] alpha 2-macroglobulin (A2M), receptor for activated C kinase (RACK), caspase (CASP), RAB7, 30,35 chitinase (CHIT), 30,32,36 ras-related C3 botulinum (RAC1), 37 mucin (MUC), chitin deacetylase, signal transducer and activator of transcription (STAT), Toll, immune deficiency (IMD), and tank-binding kinase 1 (TBK1), 32 anti-lipopolysaccharide factors (ALFs), penaeidins 3-5-13, astakine, cathepsin C (CTSC), catalase (CAT), peroxinectin, haemocyanin, glutathione peroxidase (GPX), glutathione-s-transferase (GST), superoxide dismutase (SOD), 30 lectins, β-1,3-glucan binding protein (LGBP), scavenger receptors (SRs), Down syndrome cell adhesion molecule (DSCAM), thioester-containing proteins (TEPs), serine proteinase and its homolog, 33 and ferritin (FER). 38,39 Important factors associated with processes such as apoptosis, 40,41 melanization, <...…”

“…Important factors associated with processes such as apoptosis, 40,41 melanization, 31 Toll‐like receptor (TLR) pathway, 11,40,42,43 including three major domains: a conserved region called Toll/interleukin‐1 receptor (TIR), a transmembrane area (TM) and a leucine‐rich repeat (LRR) domain, 44 as well as WNT, p38 mitogen‐activated protein kinase (MAPK), 20,40 Hippo, NOD‐like receptor, nuclear factor‐κB (NF‐κB), 20,31,43 Dorsal and Relish transcription factors, 43 IMD pathways, 31,43 VEGF signalling pathways, 45 interferon regulatory factors (IRFs), 11 Janus kinase signal transducer activator of transcription (JAK/STAT) pathways, pattern recognition receptors (PRRs), 33,46 Limpet (LMPT) 29 and antigen processing and presentation pathway, have also been identified 40 …”