The novel repressor Rce2 competes with Ace3 to regulate cellulase gene expression in the filamentous fungus <i>Trichoderma reesei</i>

Chen, Yumeng; Wang, Wei; Liu, Pei; Lin, Aibo; Fan, Xingjia; Wu, Chuan; Wei, Liujing; Wei, Dongzhi

doi:10.1111/mmi.14825

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…Recently, the biological function of a novel repressor RCE2 was characterized to repress expression of a majority of cellulase genes, and transcription factors such as cre1 , xyr1 and ace3 . A binding competition between RCE2 and ACE3 was also observed, indicating a synergetic regulation among RCE2 and other transcription factors (Chen et al 2021c ).…”

Section: Cellulase Induction Is Regulated At the Transcription Levelmentioning

confidence: 99%

From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Xiang

2021

Bioresour. Bioprocess.

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The filamentous fungus Trichoderma reesei has been widely used for cellulase production that has extensive applications in green and sustainable development. Increasing costs and depletion of fossil fuels provoke the demand for hyper-cellulase production in this cellulolytic fungus. To better manipulate T. reesei for enhanced cellulase production and to lower the cost for large-scale fermentation, it is wise to have a comprehensive understanding of the crucial factors and complicated biological network of cellulase production that could provide new perspectives for further exploration and modification. In this review, we summarize recent progress and give an overview of the cellular process of cellulase production in T. reesei, including the carbon source-dependent cellulase induction, complicated transcriptional regulation network, and efficient protein assembly and trafficking. Among that, the key factors involved in cellulase production were emphasized, shedding light on potential perspectives for further engineering.

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Section: Cellulase Induction Is Regulated At the Transcription Levelmentioning

confidence: 99%

From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Xiang

2021

Bioresour. Bioprocess.

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“…In another study, Rce2, a protein recently identified by a pull-down and mass spectrometry analysis was found to have similar binding sites in the promoter regions of target genes as Ace3, and its overexpression led to reduced cellulase expression i.e. Rce2 acted antagonistic to Ace3 in T. reesei with regards to cellulase induction and led to repressed cellulase and hemicellulase expression [ 147 ].…”

Section: Recent Developments In Identification Of Other Transcription...mentioning

confidence: 99%

Factors regulating cellulolytic gene expression in filamentous fungi: an overview

2022

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The growing demand for biofuels such as bioethanol has led to the need for identifying alternative feedstock instead of conventional substrates like molasses, etc. Lignocellulosic biomass is a relatively inexpensive feedstock that is available in abundance, however, its conversion to bioethanol involves a multistep process with different unit operations such as size reduction, pretreatment, saccharification, fermentation, distillation, etc. The saccharification or enzymatic hydrolysis of cellulose to glucose involves a complex family of enzymes called cellulases that are usually fungal in origin. Cellulose hydrolysis requires the synergistic action of several classes of enzymes, and achieving the optimum secretion of these simultaneously remains a challenge. The expression of fungal cellulases is controlled by an intricate network of transcription factors and sugar transporters. Several genetic engineering efforts have been undertaken to modulate the expression of cellulolytic genes, as well as their regulators. This review, therefore, focuses on the molecular mechanism of action of these transcription factors and their effect on the expression of cellulases and hemicellulases.

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“…In addition, Cre1 represses the expression of the activators of cellulase expression, such as Xyr1 [13]. Also, Rce1 and Rce2 are cellulase-specific repressors that compete with Xyr1 and Ace3, respectively, for binding to the cel7a promoter [14,15]. Despite extensive studies, several TFs and different regulatory mechanisms that-in a coordinated way-regulate the expression of all the arsenal to degrade the plant cell wall are still unknown.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production in Trichoderma reesei

Maués,

Maraschin,

Duarte

et al. 2023

JoF

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The fungus Trichoderma reesei is an essential producer of enzymes that degrade lignocellulosic biomass to produce value-added bioproducts. The cellulolytic system of T. reesei is controlled by several transcription factors (TFs) that efficiently regulate the production of these enzymes. Recently, a new TF named Azf1 was identified as a positive regulator of cellulase expression. Here, we investigated novel regulatory functions of Azf1 by its overexpression. In the mutant strain OEazf1, overexpression of azf1 was achieved under both repression and induction conditions. Although azf1 was more abundant in transcript and protein, overexpression of this TF did not activate transcription of the cellulase gene in the presence of the repressor glucose, suggesting that Azf1 may be subject to posttranslational regulation. In cellulose, the expression of swo, encoding the accessory protein swollenin, and the β-glucosidases cel1a, cel1b, cel3b, and cel3g increases in the early stages of cultivation. The increased production of these β-glucosidases increases the hydrolysis rate of cellobiose and sophorose, which activates carbon catabolite repression (CCR) and causes repression of cellulase genes and the regulator Xyr1 in the later stages of cultivation. Moreover, overexpression of azf1 led to increased cellulase activity in T. reesei during long-term cultivation in cellulose and sugarcane bagasse. Our results provide new insights into the mechanisms regulating Azf1 and novel genes that are important targets of this TF. This work contributes to a better understanding of the complex mechanisms regulating cellulase expression in T. reesei. It will contribute to the development of strains with higher production of these essential enzymes.

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The novel repressor Rce2 competes with Ace3 to regulate cellulase gene expression in the filamentous fungus Trichoderma reesei

Cited by 7 publications

References 45 publications

From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Factors regulating cellulolytic gene expression in filamentous fungi: an overview

Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production in Trichoderma reesei

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