The novel primers for mammal species identification-based mitochondrial cytochrome
            <i>b</i>
            sequence: implication for reserved wild animals in Thailand and endangered mammal species in Southeast Asia

Muangkram, Yuttamol; Wajjwalku, Worawidh; Amano, Atsushi; Sukmak, Manakorn

doi:10.1080/24701394.2016.1238902

Cited by 10 publications

(6 citation statements)

References 58 publications

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“…DNA meta-barcoding has been successfully applied to the illegal orchid trade as well as in the identification of endangered species in complex samples. − DNA mini-barcoding was utilized for the identification of highly processed animal skin and fur, wildlife skin samples, and samples of animal claws. − The forensic analysis of cytochrome b (cyt b), a mtDNA gene, has become an essential tool for species identification in routine practice, and for this reason, a number of different primers were designed with species identification capabilities. − Another study combined a morphometric approached with DNA analysis using cyt b and 16S rRNA genes to identify seized samples of tiger claws in India . Illegal trading of ivory is responsible for the decrease in elephant populations.…”

Section: Endangered Species and Wildlife Forensicsmentioning

confidence: 99%

Forensic DNA Analysis

McCord

Gauthier

Cho³

et al. 2018

Anal. Chem.

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Section: Endangered Species and Wildlife Forensicsmentioning

confidence: 99%

Forensic DNA Analysis

McCord

Gauthier

Cho³

et al. 2018

Anal. Chem.

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“…The suitability of quantitative PCR for wildlife research was discussed by Powell et al (2019) in their study, in which they found that quantitative RT-PCR requires minimal sample input and can analyze low-quality samples and no post-PCR analysis ensures that samples are free of contaminants. Muangkram et al (2018) identified Asian tapir by sequencing a PCR-amplified 199bp fragment of the cytochrome gene using the NCBI BLAST tool. They describe the test as a reliable and sensitive molecular assay that can be used to analyze low-quality DNA material, which may be useful in investigating wildlife crime in endangered species.…”

Section: Dna-based Methods For Wildlife Conservationmentioning

confidence: 99%

“…Muangkram et al. (2018) identified Asian tapir by sequencing a PCR‐amplified 199‐bp fragment of the cytochrome gene using the NCBI BLAST tool. They describe the test as a reliable and sensitive molecular assay that can be used to analyze low‐quality DNA material, which may be useful in investigating wildlife crime in endangered species.…”

Section: Dna‐based Methods For Wildlife Conservationmentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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“…This problem is solved by sequencing specific regions amplified with universal primers. The resulting sequences are compared with reference sequences using the BLAST search algorithm [12,13]. The SNP has become one of the most reliable markers for identifying animals from a biological sample since these mutations are numerous and widespread in the genomes of most animal species.…”

Section: Introductionmentioning

confidence: 99%

Optimization of polymerase chain reaction for the identification of Roe deer, Saiga, and Siberian stag living in Kazakhstan

et al. 2022

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Background and Aim: One of the reasons for the decline in the number of wild species of artiodactyls is poaching and the illegal trading of animal products. Molecular genetic identification of animals from a biological sample effectively proves poaching cases and illegal trade of animal products. This study aimed to develop a polymerase chain reaction (PCR) test that allows for species identification of artiodactyl animals that are most often subject to poaching. Materials and Methods: Genomic DNA was extracted from meat and blood samples of animals killed by poachers using commercial kits. Three pairs of primers were designed and used to amplify the cytochrome b gene fragment of Roe deer, Saiga antelope, and Siberian stag. Results: The proposed protocol allows amplification of specific PCR products of 542 bp with Roe deer DNA, 587 bp with Saiga DNA, and 525 bp with Siberian stag DNA. Specificity analysis showed no cross activity with DNA from other animal species. The detection limit of PCR ranged from 15.6 pg to 1.9 pg of DNA in 25 μL of the reaction mixture. Conclusion: Sequencing the amplified products and subsequent comparison with the corresponding reference sequence showed a similarity ranging from 99.99% to 100%. The PCR based on the developed primers demonstrated high sensitivity and specificity when using DNA from homogeneous and heterogeneous animals.

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The novel primers for mammal species identification-based mitochondrial cytochrome b sequence: implication for reserved wild animals in Thailand and endangered mammal species in Southeast Asia

Cited by 10 publications

References 58 publications

Forensic DNA Analysis

Forensic DNA Analysis

A systematic review of DNA‐based methods in authentication of game and less common meat species

Optimization of polymerase chain reaction for the identification of Roe deer, Saiga, and Siberian stag living in Kazakhstan

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