The novel capripoxvirus vector lumpy skin disease virus efficiently boosts modified vaccinia Ankara human immunodeficiency virus responses in rhesus macaques

Burgers, Wendy A.; Ginbot, Zekarias; Shen, Yen-Ju; Chege, Gerald K.; Soares, Andreia; Müller, Tracey L.; Bunjun, Rubina; Kiravu, Agano; Munyanduki, Henry; Douglass, Nicola; Williamson, Anna‐Lise

doi:10.1099/vir.0.067835-0

Cited by 17 publications

(21 citation statements)

References 25 publications

(30 reference statements)

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“…Based on our data we speculate that LSDV may be more suitable for a T-cell based vaccine and CNPV more suitable for the induction of an antibody response. This reflects the published data on LSDV [ 3 ] and CNPV [ 61 ].…”

Section: Discussionsupporting

confidence: 89%

“…LSDV has a host-range restricted to ruminants and is currently being investigated as an HIV vaccine vector [ 3 , 56 ]. LSDV caused the most significant response in mice compared to the other poxviruses, both in terms of the number of up-regulated genes and the magnitude and breadth of the type I Interferon response (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In a study done in Rhesus macaques rLSDV vector expressing an HIV polyprotein was immunogenic at a dose 1000-fold lower than that of rMVA. Both CD4+ and CD8+ responses were induced, rather than a predominance of CD4+ T cells observed typically for poxvirus vectors [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

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Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model

et al. 2015

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BackgroundHost-range restricted poxviruses make promising vaccine vectors due to their safety profile and immunogenicity. An understanding of the host innate immune responses produced by different poxvirus vectors would aid in the assessment, selection and rational design of improved vaccines for human and veterinary applications. Novel avipoxviruses are being assessed to determine if they are different from other poxvirus vectors. Analysis of the transcriptome induced in a mouse model would aid in determining if there were significant differences between different poxvirus vectors which may reflect different adjuvant potential as well as establish if they should be further evaluated as vaccine vectors.ResultsWe compared host transcript abundance in the spleens of BALB/c mice twenty four hours after intravenous infection (105 pfu/mouse) with six host-restricted poxvirus species from three genera, namely Lumpy Skin Disease virus (LSDV), Canarypox virus (CNPV), Fowlpox virus (FWPV), modified vaccinia Ankara (MVA) and two novel South African avipoxviruses, Feral Pigeonpox virus (FeP2) and Penguinpox virus (PEPV). These six viruses produced qualitatively and quantitatively distinct host responses with LSDV, followed by MVA, inducing the greatest interferon (IFN) response. FeP2 and PEPV caused very little change to host transcript abundance compared to the other 4 viruses tested. CNPV and FWPV induced the up regulation of two immunoglobulin genes (Ighg and Ighg3 (IgG3)) with CNPV inducing a third, Ighm (IgM). HIV-1–specific IgG3 antibodies have been correlated with decreased risk of HIV-1 infection in the RV144 trial, which included a CNPV-based vector (Yates et al. (Sci Transl Med, 6(228) p228, 2014). Up regulation of IgG3 by CNPV and FWPV but not the other poxviruses tested in vivo, implies that these two avipoxvirus-vector backbones may be involved in stimulation of the clinically important IgG3 antibody subclass. Differential transcript abundance associated with the different poxviruses is further discussed with particular emphasis on responses related to immune responses.ConclusionSix, genetically diverse host-restricted poxviruses produce different responses in a mouse model early after infection. These differences may affect the immune response induced to vaccine antigen in vectors based on these viruses. The two novel avipoxviruses were clearly distinguishable from the other viruses.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1659-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model

et al. 2015

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“…T cell activation was measured by expression of the anti-apoptotic marker, Bcl-2, and the proliferation marker, Ki67. Combined analysis of these two markers on T cells has recently been used to identify highly activated, proliferating T cells, defined as Ki67 hi Bcl-2 lo , in humans [2,[13][14][15][16] and non-human primates [17,18]. The expression kinetics of Ki-67 and Bcl-2 on CD8+ T cells mirrored those of more commonly-used activation markers, CD38 and HLA-DR, in response to yellow fever and smallpox vaccination in humans [16].…”

Section: Resultsmentioning

confidence: 85%

Transient global T cell activation after vaccination of rhesus macaques with a DNA-poxvirus vaccine regimen for HIV

Soares

Müller

Chege

et al. 2015

Vaccine

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“…Заболевание проявляется в теплое время года, в пе риод биологической активности кровососущих насе комых, быстро распространяется и протекает в виде энзоотии и эпизоотии [2,9].…”

Section: болезни крупного рогатого скота Cattle Diseases введениеunclassified

Test-System Based on Indirect “Sandwich” Elisa to Detect the Lumpy Skin Disease Virus Antigen

Рябикина¹,

Диев²,

Бьядовская³

et al. 2018

Veterinariâ segodnâ

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Lumpy skin disease is an economically significant disease as it results in decrease in weight gain and milk yield, abortions, mastitis, reproduction disorders, animal emaciation, lesions of respiratory organs and in some cases – death. Today the disease is included in the OIE list and is subject to obligatory notification. The emergence and spread of the disease in the Russian Federation necessitated performance of tests in the framework of laboratory diagnosis method improvement. The test-system based on the indirect “Sandwich” ELISA for diagnosis of lumpy skin disease allowing performance of biomaterial tests within 24 hours was for the first time developed in Russia. The test-system development included antibody preparation in laboratory animals (rabbits and guinea pigs), selection of optimal dilution of capture and detection antibodies, composition of a buffer solution and conditions of the reaction procedure. 50 samples of initial culture antigens of the lumpy skin disease virus as well virus-containing suspensions collected on different stages of purification and concentration were tested using this technique. To confirm the ELISA results all analyzed samples were tested using RT-PCR. Besides, the virus infectivity titer was determined by titration in YaDK-04 (Goat gonad cells). The test specificity was 100%, and analytical sensitivity – 3.5 lg TCD50. The developed “Sandwich” ELISA allows performing tests of 24 antigen samples at 1:2–1:16 dilution simultaneously using 96-well plate and it can be used for lumpy skin disease diagnosis.

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The novel capripoxvirus vector lumpy skin disease virus efficiently boosts modified vaccinia Ankara human immunodeficiency virus responses in rhesus macaques

Cited by 17 publications

References 25 publications

Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model

Six host-range restricted poxviruses from three genera induce distinct gene expression profiles in an in vivo mouse model

Transient global T cell activation after vaccination of rhesus macaques with a DNA-poxvirus vaccine regimen for HIV

Test-System Based on Indirect “Sandwich” Elisa to Detect the Lumpy Skin Disease Virus Antigen

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