The non‐proteolytic house dust mite allergen Der p 2 induce NF‐κB and MAPK dependent activation of bronchial epithelial cells

Österlund, Camilla; Grönlund, Hans; Polović, Natalija; Sundström, Sara; Gafvelin, Guro; Bucht, Anders

doi:10.1111/j.1365-2222.2009.03284.x

Cited by 89 publications

(83 citation statements)

References 46 publications

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“…36) This trend could be observed in the present study, because the downregulatory effect of SWCNT exposure was not observed in most of the selected genes. Moreover, the different responses observed between A549 cells and FaDu cells might be partly explained by differences in the cell differentiation, because considerable heterogeneity in the expression of heat-shock proteins, one of the best-characterized stress proteins, is observed in different tumor cells from lung carcinoma.…”

Section: Discussionsupporting

confidence: 59%

Single-Walled Carbon Nanotubes Downregulate Stress-Responsive Genes in Human Respiratory Tract Cells

Hitoshi

Katoh

Suzuki

et al. 2012

Biological & Pharmaceutical Bulletin

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Carbon nanotubes (CNTs) are currently key promising materials of nanotechnology. However, elucidation of the possible effects of CNTs on the respiratory tract is urgently needed. The present study aimed to clarify the effect of single-walled CNTs (SWCNTs) on the expression of stress-responsive genes, using primary cultured normal human bronchial epithelial (NHBE), diseased HBE (DHBE) cells, and the human carcinoma cell lines A549 and FaDu. Purified SWCNTs were applied at concentrations of 0.1 or 1.0 mg/mL for 6 h, and a polymerase chain reaction (PCR) array was conducted to examine 84 stress-responsive genes. NHBE cell exposure to SWCNTs resulted in global downregulation of genes involved in inflammation, oxidative stress, and apoptosis. Further analysis using DHBE cells and carcinoma cell lines indicated a similar trend, although differences in sensitivity were observed. Downregulation of stress-responsive genes may be involved in the mechanism by which stress response protects against lung injury.Key words carbon nanotube; stress-responsive gene; human bronchial epithelial cell Nanotechnology has drastically emerged over the past 10 years, and a vast variety of nanomaterials, which can be employed in a wide range of applications in nanoscience, medicine, and engineering, have been created. Carbon nanotubes (CNTs), discovered by Iijima, are currently key promising materials of nanotechnology.

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Section: Discussionsupporting

confidence: 59%

Single-Walled Carbon Nanotubes Downregulate Stress-Responsive Genes in Human Respiratory Tract Cells

Hitoshi

Katoh

Suzuki

et al. 2012

Biological & Pharmaceutical Bulletin

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“…TLR4 is expressed in many immune and structural cells. Indeed, Der p 2 activates dendritic cells and respiratory EC to produce pro-inflammatory mediators [21,22]. Der p 2 may trigger inflammatory responses by stimulating innate immune response in a TLR4-dependent manner and promote sensitization and development of allergic diseases.…”

Section: Toll-like Receptorsmentioning

confidence: 99%

What makes an allergen?

Scheurer

Toda

Vieths

2015

Clin Experimental Allergy

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SummaryAllergic diseases are an immune disorder reacting to certain type of allergen(s). Remarkably only a small number of proteins of the plant and animal proteome act as allergens. Therefore, allergens have been clustered according to their common structural, biochemical and functional features. Evidence has accumulated that some allergens possess intrinsic adjuvant properties to stimulate the innate immunity. The adjuvant properties appear to contribute to the allergenicity of the respective proteins, namely the ability to cause allergic sensitization in susceptible subjects or allergic reactions in sensitized individuals. Here, we discuss how allergens interact with the innate immune cells, in particular dendritic cells and epithelial cells, via binding to pattern recognition receptors, exhibiting proteolytic activities and/or inducting type 2 innate lymphoid cells (ILC2), thereby contributing to the sensitization and development of allergic diseases.

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“…Their research showed that desensitisation may cause the activation of processes related to PAR-2 receptors (protease-activated receptor) located on the surface of the respiratory epithelial cells. On the other hand, the treatment with Dermatophagoides pteronyssinus resulted in NF-kappaB and mitogen-activated protein kinase (MAPK)-dependent secretion of interleukin, cytokines, and chemokines in bronchial BEAS-2B and human bronchial epithelial cells (NHBE) [21]. In turn, Xiang et al observed the dose-dependent toxic effect of a dust mite on PHCE cell line.…”

Section: F-actinmentioning

confidence: 95%

The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549

Hałas‐Wiśniewska

Zielińska

Matulewicz

et al. 2018

Medical Research Journal

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The non‐proteolytic house dust mite allergen Der p 2 induce NF‐κB and MAPK dependent activation of bronchial epithelial cells

Abstract: These results show that Der p 2 activates respiratory epithelial cells, indicating that this non-proteolytic allergen, in addition to its immunogenic properties, can aggravate respiratory airway disease by adjuvant-like activation of the lung epithelium.

Cited by 89 publications

References 46 publications

Single-Walled Carbon Nanotubes Downregulate Stress-Responsive Genes in Human Respiratory Tract Cells

Single-Walled Carbon Nanotubes Downregulate Stress-Responsive Genes in Human Respiratory Tract Cells

What makes an allergen?

The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549

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