The Nomarski interference‐contrast microscope. An experimental basis for image interpretation*

Padawer, Jacques

doi:10.1111/j.1365-2818.1968.tb00616.x

Cited by 77 publications

(40 citation statements)

References 7 publications

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“…22 This allows episcopic illumination to produce differential interference contrast (DIC) 23,24 images, and as such enables the system to rapidly and sensitively visualize opaque or semi-opaque surfaces. Long working distance lenses allow highly convoluted and complex structures to be visualized without coverslips or oil, and remove the possibility of cross-contamination from infectious specimens.…”

Section: Microscopymentioning

confidence: 99%

Rapid method for the sensitive detection of protein contamination on surgical instruments

Lipscomb

Sihota

Botham

et al. 2006

Journal of Hospital Infection

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Section: Microscopymentioning

confidence: 99%

Rapid method for the sensitive detection of protein contamination on surgical instruments

Lipscomb

Sihota

Botham

et al. 2006

Journal of Hospital Infection

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“…Epithelial scrapings were placed in a drop of homogenizing medium on glass slides, covered with cover slips, and examined unstained in a Zeiss Photomicroscope fitted with a 40x/0.65 objective for Nomarksi interference microscopy [22,33]. The homogenates were examined by the same method at the same time.…”

Section: Morphologymentioning

confidence: 99%

Isolation of radio-iodinated apical and basal-lateral plasma membranes of toad bladder epithelium

Rodriguez

Edelman

1979

J. Membrain Biol.

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The apical and basal-lateral plasma membranes of toad bladder epithelium were radio-iodinated with the glucose-glucose oxidase-lactoperoxidase system. The covalently bound radio iodine was used as a marker during subcellular fractionation and membrane isolation. Homogenization conditions that ensured rupture of more than 80% of the cells without substantial nuclear damage were defined by Normarski optics. The nuclei were separated by differential centrifugation and the apical and basal-lateral components were resolved by differential and sucrose density gradient centrifugation. The apical components yielded two radioactive bands that were identified as glycocalyx and plasma membrane labeled with 125I. The basal-lateral components yielded a hetero-disperse pattern made up of at least 3 radioactive bands, but the bulk of the activity of ouabain-sensitive ATPase comigrated with only one of these bands. The mitochondia, identified by assays for cytochrome oxidase and NADH cytochrome c reductase activities, were separated from the radio-iodine labeled by centrifugation in sucrose density gradients under isokinetic conditions. The labeled glycocalyx and the slowly migrating components of basal-lateral labeling were separated from the radio-iodinated membranes by centrifugation at 100,000 x g x 1 hr after removal of the mitochrondria by the isokinetic method. The labeled membranes were then subjected to ultracentrifugation in sucrose density gradients under isopycnic conditions; the basal-lateral membranes containing ouabain-sensitive ATP-ase were well resolved from the apical membranes by this method. These results provide a relatively rapid method of attaining partial purification of the apical and basal-lateral plasma membranes of toad bladder epithelium.

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“…For both Protozoa and algae, we have normally used a Leitz Dialux 22 microscope equipped with differential interference contrast (Padawer, 1968). This system improves image quality, revealing a number of cellular structures, thus obviating the need for staining cells (Fig.…”

mentioning

confidence: 99%

Preservation and computer-aided microscopic analysis of planktonic Protozoa and algae

Legner

Sprules

1993

J Aquat Ecosyst Stress Recov

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Samples of microplankton and larger nanoplankton (5 to 200 gm) are preserved with a combination of Lugol's solution and DaFano's fixative. Organisms are then settled on a gelatin-coated slide, dried and embedded in 40 percent glycerin. Counting and sizing is performed under a microscope using a drawing tube, which facilitates measuring the organisms with a microcomputer-interfaced caliper. An interactive computer program, written in BASIC, allows for estimating the volumes of cells in up to 40 shape/species categories. The program then saves data on a disk, retrieves them, and calculates the results either for individual species (abundance, biomass, and mean cell volume) or as a pooled size spectrum of all organisms measured.

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The Nomarski interference‐contrast microscope. An experimental basis for image interpretation*

Cited by 77 publications

References 7 publications

Rapid method for the sensitive detection of protein contamination on surgical instruments

Rapid method for the sensitive detection of protein contamination on surgical instruments

Isolation of radio-iodinated apical and basal-lateral plasma membranes of toad bladder epithelium

Preservation and computer-aided microscopic analysis of planktonic Protozoa and algae

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