The nodD locus from Azorhizobium caulinodans is flanked by two repetitive elements

Geelen, Danny; Goethals, Koen; Montagu, Marc Van; Holsters, Marcelle

doi:10.1016/0378-1119(95)00456-g

Cited by 12 publications

(5 citation statements)

References 18 publications

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“…The shifts in GC content correspond to the location of repeated elements that are flanked by insertion sequences or tRNAs (Figures 1 and 6 ). The constitutively expressed nod D gene (AZC_3792) [ 45 , 46 ] codes for a LysR-type regulator that activates transcription of the two other flavonoid-inducible nod loci. The inducible operon nod ABCSUIJZ noe CHOP (AZC_3818-AZC_3807) [ 47 - 49 ] encodes most of the enzymatic machinery for NF backbone synthesis, decoration, and secretion.…”

Section: Resultsmentioning

confidence: 99%

The genome of the versatile nitrogen fixer Azorhizobium caulinodans ORS571

et al. 2008

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Background: Biological nitrogen fixation is a prokaryotic process that plays an essential role in the global nitrogen cycle. Azorhizobium caulinodans ORS571 has the dual capacity to fix nitrogen both as free-living organism and in a symbiotic interaction with Sesbania rostrata. The host is a fastgrowing, submergence-tolerant tropical legume on which A. caulinodans can efficiently induce nodule formation on the root system and on adventitious rootlets located on the stem.

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Section: Resultsmentioning

confidence: 99%

The genome of the versatile nitrogen fixer Azorhizobium caulinodans ORS571

et al. 2008

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“…We believe that A. caulinodans acquired its nod genes from a Bradyrhizobium, Rhizobium or Sinorhizobium strain by horizontal gene transfer because the GC content of the nod genes is lower than that of the genome and because nod genes are flanked by repetitive elements (Goethals et al, 1992a;Geelen et al, 1995b). The low GC content of the nodABCSUIJZnoeC operon extends until the border of the known sequence and no repetitive element was found in this sequence.…”

Section: Discussionmentioning

confidence: 99%

**Fucosylation and arabinosylation of Nod factors in Azorhizobium caulinodans: involvement of nolKnodZ as well as noeC and/or downstream genes**

Mergaert

D’Haeze

Fernández‐López

et al. 1996

Molecular Microbiology

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The DNA region downstream of the nodABCSUIJ operon of Azorhizobium caulinodans was further characterized and two new genes, nodZ and noeC were identified in the same operon. The A. caulinodans wild-type strain produces a population of Nod factors that, at the reducing end, are either unmodified or carry a D-arabinosyl and/or an L-fucosyl branch. Nod factors produced by Tn5-insertion mutants in nodZ, noeC, and the separate nolK locus, were analysed by thin-layer chromatography and mass spectrometry. Fucosylation of Nod factors depended on both nodZ and nolK. Arabinosylation depended on noeC and/or downstream genes. Protein extracts of A. caulinodans contained an enzymatic activity for fucose transfer from GDP-fucose to chitooligosaccharides and to Nod factors. By mutant analysis and expression of nodZ in Escherichia coli, the fucosyltransferase activity was ascribed to the protein encoded by nodZ. In addition, a Nod factor fucosyltransferase activity, independent of nodZ or other known nod genes, was detected in A. caulinodans. Finally, on the basis of sequence similarity of the nolK gene product, and mass spectrometric analysis of Nod factors produced by a nolK mutant, we propose that this gene is involved in the synthesis of GDP-fucose.

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“…Rhizobium leguminosarum and Azorhizobium caulinodans secreted by type I secretion systems were found to be cell-surface associated (Geelen et al, 1995 ;Mateos et al, 1992). Therefore, we investigated whether ExpE1 can also become attached to the outer membrane of the cell.…”

Section: Some Proteins Like Endoglycanases and Cellulases Frommentioning

confidence: 99%

The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan

et al. 2000

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In Sinorhizobium meliloti the biosynthesis of the exopolysaccharide galactoglucan (EPS II) is directed by the exp genes. The expD1 and expD2 gene products are homologous to components of type I secretion systems. ExpE1, the gene of which is located adjacent to expD1 and expD2, was detected in S. meliloti cells and culture supernatants. ExpD1 and ExpD2 were required for the secretion of ExpE1, indicating that ExpE1 is secreted by a type I secretion system involving ExpD1 and ExpD2. ExpE1 contains 15 aspartate-and glycinerich nonapeptide repeats that were suggested to bind Ca 2M . The ability to bind Ca 2M was demonstrated for a recombinant ExpE1 protein. Extracellular EPS II was not detected in cultures of non-polar expD1, expD2 and expE1 deletion mutants implying that these three genes are required for biosynthesis or secretion of galactoglucan.

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The nodD locus from Azorhizobium caulinodans is flanked by two repetitive elements

Cited by 12 publications

References 18 publications

The genome of the versatile nitrogen fixer Azorhizobium caulinodans ORS571

The genome of the versatile nitrogen fixer Azorhizobium caulinodans ORS571

**Fucosylation and arabinosylation of Nod factors in Azorhizobium caulinodans: involvement of nolKnodZ as well as noeC and/or downstream genes**

The Sinorhizobium meliloti ExpE1 protein secreted by a type I secretion system involving ExpD1 and ExpD2 is required for biosynthesis or secretion of the exopolysaccharide galactoglucan

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