The NK-2 class homeodomain factor CEH-51 and the T-box factor TBX-35 have overlapping function in<i>C. elegans</i>mesoderm development

Broitman-Maduro, Gina; Owraghi, Melissa; Hung, Wendy; Kuntz, Steven; Sternberg, Paul W.; Maduro, Morris

doi:10.1242/dev.038307

Cited by 57 publications

(89 citation statements)

References 73 publications

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“…This has been observed for lineages whose specification is well studied, such as E (Maduro and Rothman 2002), MS (Broitman-Maduro et al 2009), and C (Baugh et al 2005). We observed many new examples of this type of cascade ( Fig.…”

Section: Cascades Of Lineage-specific Expression Predict Embryonic Resupporting

confidence: 72%

Multidimensional regulation of gene expression in the C. elegans embryo

Murray¹,

Boyle²,

Preston³

et al. 2012

Genome Res.

163

247

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How cells adopt different expression patterns is a fundamental question of developmental biology. We quantitatively measured reporter expression of 127 genes, primarily transcription factors, in every cell and with high temporal resolution in C. elegans embryos. Embryonic cells are highly distinct in their gene expression; expression of the 127 genes studied here can distinguish nearly all pairs of cells, even between cells of the same tissue type. We observed recurrent lineage-regulated expression patterns for many genes in diverse contexts. These patterns are regulated in part by the TCF-LEF transcription factor POP-1. Other genes' reporters exhibited patterns correlated with tissue, position, and left–right asymmetry. Sequential patterns both within tissues and series of sublineages suggest regulatory pathways. Expression patterns often differ between embryonic and larval stages for the same genes, emphasizing the importance of profiling expression in different stages. This work greatly expands the number of genes in each of these categories and provides the first large-scale, digitally based, cellular resolution compendium of gene expression dynamics in live animals. The resulting data sets will be a useful resource for future research.

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Section: Cascades Of Lineage-specific Expression Predict Embryonic Resupporting

confidence: 72%

Multidimensional regulation of gene expression in the C. elegans embryo

Murray¹,

Boyle²,

Preston³

et al. 2012

Genome Res.

163

247

View full text Add to dashboard Cite

show abstract

“…To determine whether the time series data was of sufficient resolution and sensitivity to detect these events occurring in just a fraction of the cells, we used the change-point analysis on the unified embryonic data set to determine the peak of expression of all genes as well as the rate of change across the time course for a maximum of four intervals. The resultant data readily detected the med-1-end-3-end-1-elt-7-elt-2 cascade that specifies the intestinal fate as well as the tbx-35/pal-1-(hnd-1/hlh-1)-unc-120 cascade involved in muscle specification (Supplemental Table S7; Baugh et al 2003;Broitman-Maduro et al 2009;Raj et al 2010;Krause and Liu 2012). Thus, the data have adequate time resolution and sensitivity to order these critical events.…”

Section: Gene Expression Ordermentioning

confidence: 92%

The time-resolved transcriptome of C. elegans

et al. 2016

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We generated detailed RNA-seq data for the nematode Caenorhabditis elegans with high temporal resolution in the embryo as well as representative samples from post-embryonic stages across the life cycle. The data reveal that early and late embryogenesis is accompanied by large numbers of genes changing expression, whereas fewer genes are changing in mid-embryogenesis. This lull in genes changing expression correlates with a period during which histone mRNAs produce almost 40% of the RNA-seq reads. We find evidence for many more splice junctions than are annotated in WormBase, with many of these suggesting alternative splice forms, often with differential usage over the life cycle. We annotated internal promoter usage in operons using SL1 and SL2 data. We also uncovered correlated transcriptional programs that span >80 kb. These data provide detailed annotation of the C. elegans transcriptome.

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“…Nematodes were cultured and handled as described (Brenner, 1974 Maduro et al, 2009), LP54 PH::mCherry; NMY-2::GFP. LP53 and LP54 were constructed by crossing OD70 PH::mCherry males with PF100 GFP::MOE or JJ1473 NMY-2::GFP hermaphrodites, respectively.…”

Section: Strains and Worm Maintenancementioning

confidence: 99%

In vivo roles for Arp2/3 in cortical actin organization during C. elegans gastrulation

Roh-Johnson

Goldstein

2009

Journal of Cell Science

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The Arp2/3 complex is important for morphogenesis in various developmental systems, but specific in vivo roles for this complex in cells that move during morphogenesis are not well understood. We have examined cellular roles for Arp2/3 in the Caenorhabditis elegans embryo. In C. elegans, the first morphogenetic movement, gastrulation, is initiated by the internalization of two endodermal precursor cells. These cells undergo a myosin-dependent apical constriction, pulling a ring of six neighboring cells into a gap left behind on the ventral surface of the embryo. In agreement with a previous report, we found that in Arp2/3-depleted C. elegans embryos, membrane blebs form and the endodermal precursor cells fail to fully internalize. We show that these cells are normal with respect to several key requirements for gastrulation: cell cycle timing, cell fate, apicobasal cell polarity and apical accumulation and activation of myosin-II. To further understand the function of Arp2/3 in gastrulation, we examined F-actin dynamics in wild-type embryos. We found that three of the six neighboring cells extend short, dynamic F-actin-rich processes at their apical borders with the internalizing cells. These processes failed to form in embryos that were depleted of Arp2/3 or the apical protein PAR-3. Our results identify an in vivo role for Arp2/3 in the formation of subcellular structures during morphogenesis. The results also suggest a new layer to the model of C. elegans gastrulation: in addition to apical constriction, internalization of the endoderm might involve dynamic Arp2/3-dependent F-actin-rich extensions on one side of a ring of cells.

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The NK-2 class homeodomain factor CEH-51 and the T-box factor TBX-35 have overlapping function inC. elegansmesoderm development

Cited by 57 publications

References 73 publications

Multidimensional regulation of gene expression in the C. elegans embryo

Multidimensional regulation of gene expression in the C. elegans embryo

The time-resolved transcriptome of C. elegans

In vivo roles for Arp2/3 in cortical actin organization during C. elegans gastrulation

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