2020
DOI: 10.23986/afsci.89101
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The nitrogen-to-protein conversion factor of two cricket species - Acheta domesticus and Gryllus bimaculatus

Abstract: The Kjeldahl method is the most utilized method for total protein content analysis in food. Using the universal nitrogen-to-protein conversion factor of 6.25, the protein content of insects is likely to be overestimated due to their chitin content. We have calculated nitrogen-to-protein conversion factors for two crickets, house cricket (Acheta domesticus) and field cricket (Gryllus bimaculatus), which are used for food and feed in Europe. By analyzing their nitrogen and amino acid content we were able to show… Show more

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Cited by 40 publications
(35 citation statements)
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“…An incorrect nitrogen-to-protein conversion factor results in an overestimated protein content in edible insects [ 33 ]. Due to the exoskeleton of arthropods, composed of, inter alia, chitin, glucosamine polysaccharides, and nitrogen-rich N-acetylglucosamine [ 34 ], the use of an appropriate conversion factor is essential [ 35 ]. The fat content increased by 23%, 59%, and 105% for BCP2, BCP6, and BCP10, respectively, compared to reference bread (RB).…”
Section: Resultsmentioning
confidence: 99%
“…An incorrect nitrogen-to-protein conversion factor results in an overestimated protein content in edible insects [ 33 ]. Due to the exoskeleton of arthropods, composed of, inter alia, chitin, glucosamine polysaccharides, and nitrogen-rich N-acetylglucosamine [ 34 ], the use of an appropriate conversion factor is essential [ 35 ]. The fat content increased by 23%, 59%, and 105% for BCP2, BCP6, and BCP10, respectively, compared to reference bread (RB).…”
Section: Resultsmentioning
confidence: 99%
“…Determination of the moisture was carried out in accordance with the AACC 44-19.01 method [ 86 ]. Total nitrogen content was determined by the Kjeldahl method according to ISO 20483 [ 87 ] and was used to calculate the protein content by multiplying the result by the conversion factor of 5.7, suitable for wheat [ 88 ] and recommended by Ritvanen et al [ 89 ] for crickets. The fat content was determined (Soxhlet method) according to AACC 30-25.01 [ 90 ], and ash content according to AACC Method 08-12.01 [ 91 ].…”
Section: Methodsmentioning
confidence: 99%
“…Amino acid composition data of edible insects and meat (mg/100 g edible portion)[28,35,[43][44][45]47,48].…”
mentioning
confidence: 99%