Differences in resistance of varieties of peas to the pea aphid, Acyrthosiphon pisum (Harr.), have long ago been shown in the field and bv controlled rearings under glass, Russell and ilforrison (1924, p. 63) apparentl) being the First to observe differences. Trjrly investiprinns at Wisconsin by Searb ( 1932) showed that pea varieties differ in the number of aphids horn upon tl~ern. R~Ialtais ( 1937) corraborated these early findings and reported ( 1950) the development of a pure-line strain of apkrd-resistant pea. The influence of resistance in peas upoil aphid development, reproduction, and l o n g e v i~ was investigated by Harrington ( 1941 ) with individual plants under controlled conditions in the greenhouse. His results confirnled the jnabilitrr of the insect to develop and reproduce as fast on a resistant plant as on a susceptible one. Tt has been postulated that the composition of the available food in the host plant plays possibly the most important role in determining the resistance to aphid ittack (Evans, 1938;Painter, 1941). Maltais (1948), Auclair and !\laltais (1 9.701, and Alnltais (1951) gave preliminary reports on the probable factors in varietal resistance in peas.This is a report on the determination of total nitrogen, soluble nitrosen, and sugar contents of three susceptible varieties (Perfection, Daim-, and Lincoln) and three resistant varieties (Laurier, Champion of England, and Melting Sugar) of cultivated peas (Pisum sativum L.). Results of aphid population counts in the field (Maltais, 1937(Maltais, , 1950(Maltais, , 1951(Maltais, , and unpublished technical report, 19.50-1954 for a period of 13 years gave the following average number of aphids per plant sample for each variety: Perfection, 39.6; Ilaisy, 32.6; Lincoln, 3 5.0; Laurier (H-103), 9.8; Champion of England, 1 1.8; ~\+Ielting Sugar, 16.8.
MethodsThe plot system adopted for the growing of the pea varieties in the field was the latin square. Each plat was 15 feet long with four rows 24 inches apart. T h e plocs 11-ere separated in all directions by a space 48 inches wide.T h e seed, treated liglltlv ~v i t h Spergon (zetrachloro-pamhenzoquinone) was sown between 114ay I r a d 20. A light application of 2-12-6 fertilizer was made on tlie surface of the rows afcer seeding. T h e peas were also grown in the greenhouse, n-here the seed was treated with Spergon and placed in wet sand for germination. After one week, in earlv Februarv, rile well-developed and healthy seedlings wwe placed in loamy soif in &inch flower pots. Observations on growth were made periodicallv and plmt samples were coUected for analysis at the beginning of April. The &ages of plant growth (Table I) were established by counting the internodes of whole plants collected at random at replar intervals, the terminal bud heins counted as the first internode. Uninfested plant samples, collected from the field and the greenhouse, consisted of the temzinal growth, from the terminal bud down to the base of the fourth internode; and of the middle growth, from the fi...