The Nine Genes of the
            <i>Nocardia lactamdurans</i>
            Cephamycin Cluster Are Transcribed into Large mRNAs from Three Promoters, Two of Them Located in a Bidirectional Promoter Region

Enguita, Francisco J.; Coque, Juan José R.; Liras, Paloma; Martı́n, Juan F.

doi:10.1128/jb.180.20.5489-5494.1998

Cited by 19 publications

(10 citation statements)

References 24 publications

(19 reference statements)

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“…Coupling to the xylE reporter gene. To study if expression from latp and pcbABp was regulated by diaminopropane, DNA fragments containing each promoter (11) were coupled in the appropriate orientation to the xylE in the promoter-probe vector pIJ4083 (3).…”

Section: Methodsmentioning

confidence: 99%

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Inducing Effect of Diamines on Transcription of the Cephamycin C Genes from the lat and pcbAB Promoters in Nocardia lactamdurans

et al. 1999

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The diamines putrescine, cadaverine, and diaminopropane stimulate cephamycin biosynthesis in Nocardia lactamdurans, in shake flasks and fermentors, without altering cell growth. Intracellular levels of the P7 protein (a component of the methoxylation system involved in cephamycin biosynthesis) were increased by diaminopropane, as shown by immunoblotting studies. Lysine-6-aminotransferase and piperideine-6-carboxylate dehydrogenase activities involved in biosynthesis of the α-aminoadipic acid precursor were also greatly stimulated. The diamine stimulatory effect is exerted at the transcriptional level, as shown by low-resolution S1 protection studies. The transcript corresponding to the pcbAB gene and to a lesser extent also the lat transcript were significantly increased in diaminopropane-supplemented cultures, whereas transcription from the cefD promoter was not affected. Coupling of the lat and pcbABpromoters to the reporter xylE gene showed that expression from the lat and pcbAB promoters was increased by addition of diaminopropane in Streptomyces lividans. Intracellular accumulation of diamines in Nocardia may be a signal to trigger antibiotic production.

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Section: Methodsmentioning

confidence: 99%

“…The N. lactamdurans genes encoding cephamycin biosynthesis enzymes have been cloned and sequenced and shown to be clustered (6) in a 30-kb region of the genome of this microorganism (22). The genes of the cephamycin cluster are expressed as polycistronic mRNAs from three promoters, latp, pcbABp, and cefDp (11).…”

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confidence: 99%

Inducing Effect of Diamines on Transcription of the Cephamycin C Genes from the lat and pcbAB Promoters in Nocardia lactamdurans

et al. 1999

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“…Genes belonging to the same metabolic pathway are very often found clustered and organized in operons in bacterial genomes. Examples of gene clusters resulting in large polycistronic transcripts 17.5 and 16 kb have been previously described in Clostridium difficile (Hammond et al, 1997) and Nocardia lactamdurans (Enguita et al, 1998) respectively. This is also true for the genes involved in photosynthesis (PS).…”

Section: Introductionmentioning

confidence: 84%

Organization and expression of photosynthesis genes and operons in anoxygenic photosynthetic proteobacteria

Liotenberg

Steunou

Picaud

et al. 2008

Environmental Microbiology

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Genes belonging to the same metabolic route are usually organized in operons in microbial genomes. For instance, most genes involved in photosynthesis were found clustered and organized in operons in photosynthetic Alpha- and Betaproteobacteria. The discovery of Gammaproteobacteria with a conserved photosynthetic gene cluster revives the questions on the role and the maintenance of such organization in proteobacteria. In this paper, we report the analysis of the structure and expression of the 14 kb cluster (crtEF-bchCXYZ-pufBALMC-crtADC) in the photosynthetic betaproteobacterium Rubrivivax gelatinosus, with the purpose of understanding the reasons and the biological constraints that might have led to the clustering of photosynthesis genes. The genetic analyses are substantiated by reverse transcription-PCR data which reveal the presence of a transcript encompassing the 14 genes and provide evidence of a polycistronic 'super-operon' organization starting at crtE and ending 14 kb downstream at the crtC gene. Furthermore, genetic analyses suggest that one of the selection pressures that may have driven and maintained the photosynthesis operons/super-operons in proteobacteria could very likely be the coexpression and regulation of the clustered genes/operon.

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“…The ribosome slow-down may detach the RNA polymerase from the DNA reducing expression of the ceaS2 gene and modulating expression of the ccaR and claR genes. Attenuation of antibiotic resistance genes as a mechanism of control of gene expression was proposed a few years ago by Weisblum and coworkers (Mayford and Weisblum, 1990;Kwak et al, 1991) but this mechanism has not been studied in detail, although mechanisms of attenuation (termination/ antitermination) were proposed to explain the different overlapping transcripts of the cephamycin gene cluster (Enguita et al, 1998). The rplK gene is linked to nusG encoding a transcriptional antiterminator-like protein in different Streptomyces species and is, at least in part, cotranscribed with nusG (J.P. Gomez-Escribano, unpubl.…”

Section: Discussionmentioning

confidence: 99%

An rplK^{Δ29‐PALG‐32} mutation leads to reduced expression of the regulatory genes ccaR and claR and very low transcription of the ceaS2 gene for clavulanic acid biosynthesis in Streptomyces clavuligerus

Gomez-Escribano¹,

Liras²,

Pisabarro³

et al. 2006

Molecular Microbiology

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An rplK SummaryThe transcriptional and translational control of the biosynthesis of the b-lactamase inhibitor clavulanic acid is a subject of great scientific and industrial interest. To study the role of the ribosomal protein L11 on control of clavulanic acid gene transcription, the DNA region aspC-tRNA trp -secE-rplK-rplA-rplJ-rplL of Streptomyces clavuligerus was cloned and characterized. An S. clavuligerus rplK DPALG mutant, with an internal 12 nucleotides in-frame deletion in the rplK gene, encoding the L11 (RplK) ribosomal protein lacking amino acids 29 PALG 32 , was constructed by gene replacement. This deletion alters the L11 N-terminal domain that interacts with the RelA and class I releasing factors-mediated translational termination. The mutant grew well, showed threefold higher resistance to thiostrepton, did not form spores and lacked diffusible brown pigments, as compared with the wild-type strain. The wild-type phenotype was recovered by complementation with the native rplK gene. S. clavuligerus rplK DPALG produced reduced levels of clavulanic acid (15-26% as compared with the wild type) and cephamycin C (40-50%) in cultures grown in defined SA and complex TSB media. The decreased yields resulted from an impaired transcription of the regulatory genes ccaR and claR and the cefD and ceaS2 genes for cephamycin and clavulanic acid biosynthesis respectively. Expression of ceaS2 encoding carboxyethylarginine synthase (CEAS), the precursor-committing enzyme for clavulanic acid biosynthesis, was particularly affected in this mutant. In the wild-type strain polyphosphorylated nucleotides peaked at 36-48 h of growth in SA cultures whereas expression of the cephamycin and clavulanic acid genes occurred 12-24 h earlier than the increase in ppGpp indicating that there is no strict correlation between the peak of ppGpp and the onset of transcription of the clavulanic acid and cephamycin C biosynthesis. The drastic effect of the rplK DPALG mutation on the onset of expression of the ceaS2 and the regulatory ccaR and claR genes and the lack of correlation with ppGpp levels suggest that the onset of transcription of these genes is modulated by the conformational alteration of the N-terminal region of L11 probably by interaction with the nascent peptide releasing factors and with RelA.

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The Nine Genes of the Nocardia lactamdurans Cephamycin Cluster Are Transcribed into Large mRNAs from Three Promoters, Two of Them Located in a Bidirectional Promoter Region

Cited by 19 publications

References 24 publications

Inducing Effect of Diamines on Transcription of the Cephamycin C Genes from the lat and pcbAB Promoters in Nocardia lactamdurans

Inducing Effect of Diamines on Transcription of the Cephamycin C Genes from the lat and pcbAB Promoters in Nocardia lactamdurans

Organization and expression of photosynthesis genes and operons in anoxygenic photosynthetic proteobacteria

An rplK^{Δ29‐PALG‐32} mutation leads to reduced expression of the regulatory genes ccaR and claR and very low transcription of the ceaS2 gene for clavulanic acid biosynthesis in Streptomyces clavuligerus

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The Nine Genes of the Nocardia lactamdurans Cephamycin Cluster Are Transcribed into Large mRNAs from Three Promoters, Two of Them Located in a Bidirectional Promoter Region

Cited by 19 publications

References 24 publications

Inducing Effect of Diamines on Transcription of the Cephamycin C Genes from the lat and pcbAB Promoters in Nocardia lactamdurans

Inducing Effect of Diamines on Transcription of the Cephamycin C Genes from the lat and pcbAB Promoters in Nocardia lactamdurans

Organization and expression of photosynthesis genes and operons in anoxygenic photosynthetic proteobacteria

An rplKΔ29‐PALG‐32 mutation leads to reduced expression of the regulatory genes ccaR and claR and very low transcription of the ceaS2 gene for clavulanic acid biosynthesis in Streptomyces clavuligerus

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An rplK^{Δ29‐PALG‐32} mutation leads to reduced expression of the regulatory genes ccaR and claR and very low transcription of the ceaS2 gene for clavulanic acid biosynthesis in Streptomyces clavuligerus