The Nicotiana tabacum L. major latex protein-like protein 423 (NtMLP423) positively regulates drought tolerance by ABA-dependent pathway

Yuan²,

Xing³

et al. 2023

Front. Plant Sci.

The HVA22 family of genes, induced by abscisic acid and stress, encodes a class of stress response proteins with a conserved TB2/DP1/HVA22 domain that are unique among eukaryotes. Previous studies have shown that HVA22s play an important role in plant responses to abiotic stresses. In the present study, 34, 32, 16, and 17 HVA22s were identified in G. barbadense, G. hirsutum, G. arboreum, and G. raimondii, respectively. These HVA22 genes were classified into nine subgroups, randomly distributed on the chromosomes. Synteny analysis showed that the amplification of the HVA22s were mainly due to segmental duplication or whole genome replication (WGD). Most HVA22s promoter sequences contain a large number of drought response elements (MYB), defense and stress response elements (TC-rich repeats), and hormone response elements (ABRE, ERE, SARE, etc.), suggesting that HVA22s may respond to adversity stresses. Expression profiling demonstrated that most GhHVA22s showed a constitutive expression pattern in G. hirsutum and could respond to abiotic stresses such as salt, drought, and low temperature. Overexpression of GhHVA22E1D (GH_D07G0564) in Arabidopsis thaliana enhances salt and drought tolerance in Arabidopsis. Virus-induced gene silencing of GhHVA22E1D reduced salt and drought tolerance in cotton. This indicates that GhHVA22E1D plays an active role in the plant response to salt stress and drought stress. GhHVA22E1D may act in plant response to adversity by altering the antioxidant capacity of plants. This study provides valuable information for the functional genomic study of the HVA22 gene family in cotton. It also provides a reference for further elucidation of the functional studies of HVA22 in plant resistance to abiotic stress response.

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the HVA22 gene family in cotton and functional analysis of GhHVA22E1D in drought and salt tolerance

Yuan²,

Xing³

et al. 2023

Front. Plant Sci.

“…It has been reported that overexpression of the AtMLP43 gene can mediate ABA signaling to improve drought tolerance [31]. Overexpression in transgenic tobacco plants of the major latex-like protein gene NtMLP423 resulted in greater sensitivity to abscisic acid (ABA)-mediated seed germination and ABA-induced stomatal closure, and enhanced drought tolerance in tobacco by increasing ABA levels [32]. Based on this, we speculated that the expression of ABA-responsive genes might be altered in overexpressed plants.…”

Section: Discussionmentioning

confidence: 91%

OsMLP423 is a positive regulator of tolerance to drought and salt stress in rice

Zhou

Fan

et al. 2022

Preprint

Drought and salinity is one of the important abiotic factors that adversely affects plant growth and production.We screened a drought-inducing gene containing about 158 amino acid residues from the transcriptome library of rice exposed to drought treatment, and found ABA-related cis-acting elements and multiple drought-stress-related cis-acting elements in its promoter sequence. The physiological and biochemical phenotype analysis of transgenic plants confirmed that overexpressed OsMLP423 enhanced the tolerance of rice to drought and salt stress. The expression of the OsMLP423-GFP fusion protein showed that OsMLP423 was localized in both the cell membrane and nucleus. Compared with the wild-type, the plants over-expressing OsMLP423 showed the enhanced sensitivity to ABA. These results indicate that OsMLP423 is a positive regulator of drought and salinity tolerance in rice, governing the tolerance of rice to abiotic stress through an ABA-dependent pathway. This paper provides basic knowledge on the drought- and salt-tolerant transgenic rice with a potential application value.

“…When exposed to the osmotic adjustment substance PEG or a water deficit, overexpression lines showed greater tolerance to water stress and had significant plant heights, survival rates, and water retention statuses [43]. It was reported that overexpression in transgenic tobacco plants of the major latex-like protein gene NtMLP423 resulted in greater sensitivity to abscisic acid (ABA)-mediated seed germination and ABA-induced stomatal closure, and it enhanced drought tolerance in tobacco by increasing endogenous ABA levels [44]. Ectopic overexpression of the cotton GhMLP28 gene in Arabidopsis enhanced tolerance to salt stress [45].…”

Section: Discussionmentioning

confidence: 99%

OsMLP423 Is a Positive Regulator of Tolerance to Drought and Salt Stresses in Rice

Zhou

Fan²,

et al. 2022

Plants

Rice (Oryza sativa L.) is one of the main food crops for human survival, and its yield is often restricted by abiotic stresses. Drought and soil salinity are among the most damaging abiotic stresses affecting today’s agriculture. Given the importance of abscisic acid (ABA) in plant growth and abiotic stress responses, it is very important to identify new genes involved in ABA signal transduction. We screened a drought-inducing gene containing about 158 amino acid residues from the transcriptome library of rice exposed to drought treatment, and we found ABA-related cis-acting elements and multiple drought-stress-related cis-acting elements in its promoter sequence. The results of real-time PCR showed that OsMLP423 was strongly induced by drought and salt stresses. The physiological and biochemical phenotype analysis of transgenic plants confirmed that overexpression of OsMLP423 enhanced the tolerance to drought and salt stresses in rice. The expression of OsMLP423-GFP fusion protein indicated that OsMLP423 was located in both the cell membrane system and nucleus. Compared with the wild type, the overexpressed OsMLP423 showed enhanced sensitivity to ABA. Physiological analyses showed that the overexpression of OsMLP423 may regulate the water loss efficiency and ABA-responsive gene expression of rice plants under drought and salt stresses, and it reduces membrane damage and the accumulation of reactive oxygen species. These results indicate that OsMLP423 is a positive regulator of drought and salinity tolerance in rice, governing the tolerance of rice to abiotic stresses through an ABA-dependent pathway. Therefore, this study provides a new insight into the physiological and molecular mechanisms of OsMLP423-mediated ABA signal transduction participating in drought and salt stresses.