The NF-YA transcription factor OsNF-YA7 confers drought stress tolerance of rice in an abscisic acid independent manner

Lee, Dong-Keun; Kim, Hyung Il; Jang, Geupil; Chung, Phil Hyun; Jeong, Jena; Kim, Youn Shic; Bang, Seung Woon; Jung, Harin; Choi, Yang Do; Kim, Ju-Kon

doi:10.1016/j.plantsci.2015.10.006

Cited by 92 publications

(71 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These target genes belong to different clades in a phylogenetic tree. While Os02g53620 ( NF-YA11 ) is not reported before, Os03g07880 ( NF-YA1 ), Os03g29760 ( NF-YA2 ), and Os07g41720 ( NF-YA6 ) are classified into class 1 clade, Os03g48970 ( NF-YA4 ) belongs to class 2, and Os03g44540 ( NF-YA3 ) and Os12g42400 ( NF-YA10 ) belong to class 3 clade (Petroni et al, 2012; Lee et al, 2015). It is interesting that the genes in the same clade displayed similar transcription patterns upon M. oryzae infection in LTH and IRBL9-W, suggesting that the NF-YAs in the different clade may play different roles in rice immunity against M. oryzae .…”

Section: Discussionmentioning

confidence: 77%

“…Os03g29760 ( NF-YA2 ) and Os07g41720 ( NF-YA6 ) are rapidly down-regulated upon high salinity treatment, while the other NF-YA genes are not obviously changed (Zhao et al, 2009), indicating different NF-YA genes are differentially responsive to salt stress. In addition, different NF-YA genes are differentially induced by drought, salt and temperature stresses, but only OsNF-YA7 ( Os08g09690 ), a non-target of miR169, is highly induced by drought and salt treatment (Lee et al, 2015). In the present study, seven target NF-YA genes of miR169a were also differentially responsive to M. oryzae infection in LTH and IRBL9-W ( Figure 7A ).…”

Section: Discussionmentioning

confidence: 99%

“…In wheat, the expression of miR169 is reduced, whereas the expression of NF-YAs , are induced by N and P starvation (Qu et al, 2015). In rice, OsNF-YA7 is induced by drought stress and the transgenic plants overexpressing OsNF-YA7 exhibit ABA-independent tolerance to drought stress (Lee et al, 2015). These literatures indicate that miR169/NF-YA modules regulate tolerance to abiotic stresses in both monocots and dicots.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Osa-miR169 Negatively Regulates Rice Immunity against the Blast Fungus Magnaporthe oryzae

et al. 2017

View full text Add to dashboard Cite

miR169 is a conserved microRNA (miRNA) family involved in plant development and stress-induced responses. However, how miR169 functions in rice immunity remains unclear. Here, we show that miR169 acts as a negative regulator in rice immunity against the blast fungus Magnaporthe oryzae by repressing the expression of nuclear factor Y-A (NF-YA) genes. The accumulation of miR169 was significantly increased in a susceptible accession but slightly fluctuated in a resistant accession upon M. oryzae infection. Consistently, the transgenic lines overexpressing miR169a became hyper-susceptible to different M. oryzae strains associated with reduced expression of defense-related genes and lack of hydrogen peroxide accumulation at the infection site. Consequently, the expression of its target genes, the NF-YA family members, was down-regulated by the overexpression of miR169a at either transcriptional or translational level. On the contrary, overexpression of a target mimicry that acts as a sponge to trap miR169a led to enhanced resistance to M. oryzae. In addition, three of miR169’s target genes were also differentially up-regulated in the resistant accession upon M. oryzae infection. Taken together, our data indicate that miR169 negatively regulates rice immunity against M. oryzae by differentially repressing its target genes and provide the potential to engineer rice blast resistance via a miRNA.

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Osa-miR169 Negatively Regulates Rice Immunity against the Blast Fungus Magnaporthe oryzae

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Rice (Oryza sativa L.) is one of the world's most important food crops as well as a model species for monocot molecular biology investigation (Itoh et al, 2005). In rice, various NF-Y subunits are involved in specific developmental processes or responses to developmental signals, including seed nutrient accumulation, flowering time regulation, ABA response and chloroplast development (Lee et al, 2015;Li et al, 2015;Romier et al, 2003;Siefers et al, 2009;Sinha et al, 1995;Xu et al, 2016;Yamamoto et al, 2009). Rice genome contains 10 NF-YA, 11 NF-YB and 12 NF-YC genes, among which NF-YBs are the most well-documented subunits (Gusmaroli et al, 2001;Petroni et al, 2012;Thirumurugan et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

Bello

Hou

Zhao

et al. 2019

Plant Biotechnology Journal

109

103

View full text Add to dashboard Cite

Summary Identification of seed development regulatory genes is the key for the genetic improvement in rice grain quality. NF ‐Ys are the important transcription factors, but their roles in rice grain quality control and the underlying molecular mechanism remain largely unknown. Here, we report the functional characterization a rice NF ‐Y heterotrimer complex NF ‐ YB 1‐ YC 12‐ bHLH 144, which is formed by the binding of NF ‐ YB 1 to NF ‐ YC 12 and then bHLH 144 in a sequential order. Knock‐out of each of the complex genes resulted in alteration of grain qualities in all the mutants as well as reduced grain size in crnf‐yb1 and crnf‐yc12 . RNA ‐seq analysis identified 1496 genes that were commonly regulated by NF ‐ YB 1 and NF ‐ YC 12 , including the key granule‐bound starch synthase gene Wx . NF ‐ YC 12 and bHLH 144 maintain NF ‐ YB 1 stability from the degradation mediated by ubiquitin/26S proteasome, while NF ‐ YB 1 directly binds to the ‘G‐box’ domain of Wx promoter and activates Wx transcription, hence to regulate rice grain quality. Finally, we revealed a novel grain quality regulatory pathway controlled by NF ‐ YB 1‐ YC 12‐ bHLH 144 complex, which has great potential for rice genetic improvement.

show abstract

“…Modifying their activity may provide a means to increase the capacity of a plant to avoid drought stress. For example, a number of TFs from rice (Oryza sativa) have been shown to enhance drought tolerance using an overexpression strategy (Hu et al, 2006;Jeong et al, 2010Jeong et al, , 2013Lee et al, 2015Lee et al, , 2016Lee et al, , 2017Oh et al, 2009;Park et al, 2015;Redillas et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of OsTF1L, a rice HD‐Zip transcription factor, promotes lignin biosynthesis and stomatal closure that improves drought tolerance

Bang

Lee

Jung

et al. 2018

Plant Biotechnology Journal

Self Cite

110

View full text Add to dashboard Cite

Drought stress seriously impacts on plant development and productivity. Improvement of drought tolerance without yield penalty is a great challenge in crop biotechnology. Here, we report that the rice (Oryza sativa) homeodomain-leucine zipper transcription factor gene, OsTF1L (Oryza sativa transcription factor 1-like), is a key regulator of drought tolerance mechanisms. Overexpression of the OsTF1L in rice significantly increased drought tolerance at the vegetative stages of growth and promoted both effective photosynthesis and a reduction in the water loss rate under drought conditions. Importantly, the OsTF1L overexpressing plants showed a higher drought tolerance at the reproductive stage of growth with a higher grain yield than nontransgenic controls under field-drought conditions. Genomewide analysis of OsTF1L overexpression plants revealed up-regulation of drought-inducible, stomatal movement and lignin biosynthetic genes. Overexpression of OsTF1L promoted accumulation of lignin in shoots, whereas the RNAi lines showed opposite patterns of lignin accumulation. OsTF1L is mainly expressed in outer cell layers including the epidermis, and the vasculature of the shoots, which coincides with areas of lignification. In addition, OsTF1L overexpression enhances stomatal closure under drought conditions resulted in drought tolerance. More importantly, OsTF1L directly bound to the promoters of lignin biosynthesis and drought-related genes involving poxN/PRX38, Nodulin protein, DHHC4, CASPL5B1 and AAA-type ATPase. Collectively, our results provide a new insight into the role of OsTF1L in enhancing drought tolerance through lignin biosynthesis and stomatal closure in rice.

show abstract

The NF-YA transcription factor OsNF-YA7 confers drought stress tolerance of rice in an abscisic acid independent manner

Cited by 92 publications

References 70 publications

Osa-miR169 Negatively Regulates Rice Immunity against the Blast Fungus Magnaporthe oryzae

Osa-miR169 Negatively Regulates Rice Immunity against the Blast Fungus Magnaporthe oryzae

NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

Overexpression of OsTF1L, a rice HD‐Zip transcription factor, promotes lignin biosynthesis and stomatal closure that improves drought tolerance

Contact Info

Product

Resources

About