The new vertebrate CYP1C family: Cloning of new subfamily members and phylogenetic analysis

Godard, Céline A. J.; Goldstone, Jared V.; Said, Maya R.; Dickerson, R.; Woodin, Bruce R.; Stegeman, John J.

doi:10.1016/j.bbrc.2005.03.231

Cited by 63 publications

(57 citation statements)

References 38 publications

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“…Here we present a comprehensive analysis of the expression of the CYP1s in the zebrafish model, including the recently discovered CYP1Cs, showing that CYP1A, CYP1B1, CYP1C1 and CYP1C2 all are expressed in multiple organs of control adult zebrafish, and also appear to be constitutively expressed in zebrafish embryos. CYP1A has been widely studied in fish (e.g., Arinç et al, 2000) but there are very few studies of fish CYP1B (Leaver and George, 2000;El-kady et al, 2004b;El-kady et al, 2004a; or the CYP1Cs (Godard et al, 2005;Itakura et al, 2005;Wang et al, 2006). Our results showing appreciable basal levels of expression of the CYP1Cs and CYP1B1 in some organs and in embryos, suggest that there are endogenous roles for the CYP1B1 and CYP1Cs.…”

Section: Discussionmentioning

confidence: 54%

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Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Jönsson

Orrego

Woodin

et al. 2007

Toxicology and Applied Pharmacology

133

143

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The cytochrome P4501C (CYP1C) gene subfamily was recently discovered in fish, and zebrafish (Danio rerio) CYP1C1 transcript has been cloned. Here we cloned the paralogous CYP1C2, showing that the amino acid sequence is 78% identical to CYP1C1, and examined gene structure and expression of CYP1A, CYP1B1, CYP1C1, and CYP1C2. Xenobiotic response elements were observed upstream of the coding regions in all four genes. Zebrafish adults and embryos were exposed (24 hours) to 100 nM 3,3',4,4',5-polychlorinated biphenyl (PCB126) or 20 ppm acetone and subsequently held in clean water for 24 hours (adults) or 48 hours (embryos). All adult organs examined (eye, gill, heart, liver, kidney, brain, gut, and gonads) and embryos showed basal expression of the four genes. CYP1A was most strongly expressed in liver, whereas CYP1B1, CYP1C1, and CYP1C2 were most strongly expressed in heart and eye. CYP1B1 and the CYP1C genes showed an expression pattern similar to one another and to mammalian CYP1B1. In embryos CYP1C1 and CYP1C2 tended to have a higher basal expression than CYP1A and CYP1B1. PCB126 induced CYP1A in all organs, and CYP1B1 and CYP1C1 in all organs except gonads, or gonads and brain, respectively. CYP1C2 induction was significant only in the liver. However, in embryos all four genes were induced strongly by PCB126. The results are consistent with CYP1C1 and CYP1C2, as well as CYP1A and CYP1B1, being regulated by the aryl hydrocarbon receptor. While CYP1A may have a protective role against AHR agonists in liver and gut, CYP1B1, CYP1C1, and CYP1C2 may also play endogenous roles in eye and heart and possibly other organs, as well as during development.

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Section: Discussionmentioning

confidence: 54%

“…In parallel, we repeated the cloning and sequencing of CYP1C1 from both TL and PS fish, using primers based on the CYP1C1 sequence published by Godard et al (2005). The aligned zebrafish TL CYP1C1 and CYP1C2 protein sequences (Fig 1) had a 78 % amino acid sequence identity.…”

Section: Cloned Cyp1c1 and Cyp1c2 Transcriptsmentioning

confidence: 99%

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Jönsson

Orrego

Woodin

et al. 2007

Toxicology and Applied Pharmacology

133

143

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“…Possibly another P450 isoform, also induced by 3-MC and susceptible to inhibition by ␣-naphthoflavone, was responsible for the increased production of OH-MXC. Multiple CYP1 family isoforms in the 1B and 1C subfamilies have been cloned from other fish species (Godard et al, 2005). If present in catfish, one of these other CYP1 family isoforms may metabolize methoxychlor.…”

Section: Discussionmentioning

confidence: 99%

DEMETHYLATION OF THE PESTICIDE METHOXYCHLOR IN LIVER AND INTESTINE FROM UNTREATED, METHOXYCHLOR-TREATED, AND 3-METHYLCHOLANTHRENE-TREATED CHANNEL CATFISH (ICTALURUS PUNCTATUS): EVIDENCE FOR ROLES OF CYP1 AND CYP3A FAMILY ISOZYMES

Stuchal¹,

Kleinow²,

Stegeman³

et al. 2006

Drug Metab Dispos

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“…CYP1Cs are found in all non-mammalian vertebrate lineages (Goldstone et al 2007). Fish have CYP1C1 and CYP1C2 paralogs arising from a gene duplication event (Godard et al 2005, Goldstone et al 2007. The CYP1D subfamily, a non-mammalian subfamily which has been recently identified, contains a single CYP1D1 gene (Goldstone et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P450-mediated 17β-estradiol metabolism in zebrafish (Danio rerio)

Scornaienchi

Thornton²,

Willett³

et al. 2010

Journal of Endocrinology

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Cytochrome P4501 (CYP1) and CYP3A proteins are primarily responsible for the metabolism of 17b-estradiol (E 2 ) in mammals. We have cloned and heterologously expressed CYP1A, CYP1B1, CYP1C1, CYP1C2, CYP1D1, and CYP3A65 from zebrafish (Danio rerio) to determine the CYP-mediated metabolism of E 2 in a non-mammalian species. Constructs of each CYP cDNA were created using a leader sequence from the bacterial ompA gene to allow appropriate expression in Escherichia coli without 5 0 modification of the gene. Membrane vesicles were purified, and functional CYP protein was verified using carbon monoxide difference spectra and fluorescent catalytic assays with the substrates 7-ethoxyresorufin and 7-benzyloxy-4-(trifluoromethyl)-coumarin. Rates of in vitro E 2 metabolism into 4-hydroxyE 2 (4-OHE 2 ), 2-hydroxyE 2 (2-OHE 2 ), and 16a-hydroxyE 1 (16a-OHE 1 ) metabolites were determined by gas chromatography/mass spectrometry. The 2-OHE 2 metabolite was produced by all CYPs tested, while 4-OHE 2 was only detected following incubation with CYP1A, CYP1B1, CYP1C1, and CYP1C2. The 16a-OHE 1 metabolite was only produced by CYP1A. The highest rates of E 2 metabolism were from CYP1A and CYP1C1, followed by CYP1C2. CYP1B1, CYP1D1, and CYP3A65 had low rates of E 2 metabolism. E 2 metabolism by zebrafish CYP1A, CYP1C1, and CYP1C2 produced similar ratios of 4-OHE 2 to 2-OHE 2 as previous studies with mammalian CYP1As. CYP1B1 formed the highest ratio of 4-OHE 2 to 2-OHE 2 metabolites. Contrary to mammals, these results suggest that fish CYP1A and CYP1C proteins are primarily responsible for E 2 metabolism, with only minor contributions from CYP3A65 and CYP1B1. Similar to mammals, 2-OHE 2 is the predominant metabolite from CYP-mediated E 2 metabolism in fish, suggesting that all vertebrate species produce the same major E 2 metabolite.

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The new vertebrate CYP1C family: Cloning of new subfamily members and phylogenetic analysis

Cited by 63 publications

References 38 publications

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

DEMETHYLATION OF THE PESTICIDE METHOXYCHLOR IN LIVER AND INTESTINE FROM UNTREATED, METHOXYCHLOR-TREATED, AND 3-METHYLCHOLANTHRENE-TREATED CHANNEL CATFISH (ICTALURUS PUNCTATUS): EVIDENCE FOR ROLES OF CYP1 AND CYP3A FAMILY ISOZYMES

Cytochrome P450-mediated 17β-estradiol metabolism in zebrafish (Danio rerio)

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