The new sheep ovarian cell culture and the prospect its application in virology

Abstract

The new sheep ovarian cell culture named as SO was derived and its reproduction has been successful carried out using available growth mediums such as mixture of modified Eagle’s medium, lactalbumin hydrolysate and bovine serum. The monolayer was formed at a seeding concentration of 100-120 thousand cells/ml in 3-5 days. The proliferation index was 2.81-3.24. Fibroblast-like cells predominated at early generations of the culture. During high passages, domination of epithelial-like cells was observed. While passaging, the modal class of 54 chromosomes was retained but the number of chromosome dispersion increased and the number of cells with a diploid set decreased. The cell viability was 82-75% during long-term storage in liquid nitrogen. The monolayer was retained for at least two weeks without changing the medium at 37°C. The culture produced estradiol and progesterone. Cell culture was highly sensitive to bovine herpesvirus 1, parainfluenza-3, nodular dermatitis and pustular stomatitis (Orf) viruses, but had low sensitivity to Orthopneumovirus. It didn’t allow reproduction of pestivirus – causative agent of bovine viral diarrhea – mucosal disease.