The new ophthalmic formulation for infection control by combining collagen/gelatin/alginate biomaterial with liposomal chloramphenicol

Abstract

Eye drops are a conventional method of drug delivery to the eye, accounting for 90% of currently accessible ophthalmic formulations. The major problem with eye drop treatments is rapid pre-corneal drug loss. Furthermore, the need for frequent administration of eye drops can profoundly affect the quality of life of ophthalmological patients. In the current study, we developed a liposomal nanoparticle encapsulated with chloramphenicol mixed with biodegradable materials against ophthalmological disease. We first established a protocol for chloramphenicol (CAP) loaded into liposomal nanoparticle (LipoCAP). We also established the collagen/gelatin/sodium alginate (CGA) as the component of biodegradable polymers and calibrated the novel drug-releasing formulation. Finally, we combined LipoCAP with CGA to generate an 8-h degradable ophthalmic chloramphenicol gel, CGA-LipoCAP-8. CGA-LipoCAP-8 reached the effective working concentration in 75 min and prolonged the drug-releasing time for at least 12 h. In addition, CGA-LipoCAP-8 could stably and continuously inhibit E. coli proliferation. The inhibiting phenomenon was more pronounced over time. Furthermore, there were no significant toxicities observed when CGA-LipoCAP-8 co-cultured with ocular epithelial cells. In conclusion, CGA-LipoCAP-8 achieved effective CAP dose concentrations in a short time and sustained CAP release for a prolonged period. Our results provide an innovative concept in relation to novel drug-release formulations, with safety and efficiency supporting use in future treatments for ophthalmological diseases.

Polymeric hydrogel as a vitreous substitute: current research, challenges, and future directions

“…Alginate is an anionic copolymer with Lguluronic acid and D-mannuronic blocks acid [93], which has been also extensively used to construct biocompatible hydrogels. Some studies demonstrated that alginate might induce myocardial repair and regeneration, and stem cell delivery [94,95]. Alginate could quickly react to divalent cations (e.g.…”

Liposomal dexamethasone–moxifloxacin nanoparticle combinations with collagen/gelatin/alginate hydrogel for corneal infection treatment and wound healing

“…Our first step at this stage was to establish the appropriate formulation of CGA-Lipo-MFX/DEX for ophthalmic use. Based on our degradation curve measurement, we developed a formula to determine the relationship between the molar ratio of sodium citrate to sodium alginate with the CGA decomposition time: Y = 12.3314X 2 − 38.6691X + 29.2180 (where Y is the CGA decomposition time, and X is the molar ratio of sodium citrate to sodium alginate) [24]. Based on this degradation formula, we designed an 8 h degradable formulation by adjusting the sodium citrate/sodium alginate ratio in our investigation.…”

“…Based on our previous investigation, we established the formula to determine the relationship between the molar ratio of sodium citrate to sodium alginate with CGA decomposition time [24]. The CGA complex completely degraded in 0.01% sodium citrate.…”

“…The use of the conventional liposomal formulation or hydrogel that is applied in the case of ophthalmological illnesses and especially in that of infective diseases has been investigated for decades [10,22,23]. In our previous study, a novel ophthalmic formulation was developed by combining a collagen/gelatin/alginate (CGA) biomaterial with liposomal chloramphenicol in order to control infection [24]. In the current study, we aimed to develop a delivery system consisting of a dexamethasone (DEX) and MFX encapsulated liposomal nanoparticle-containing biodegradable film (CGA-Lipo-MFX/DEX) for application in ophthalmological disease treatment.…”

Microneedle-based ocular drug delivery systems – recent advances and challenges

“…Therefore, apart from designing the vehicle/base composition, drugs are often incorporated into appropriate carriers or introduced into systems whose purpose is to provide the expected concentration in the treated tissue for the desired time period. The most frequently studied and described are liposomes [60,61], micelles [60,62], microparticles [63][64][65], nanoparticles [66,67], micro- [68,69], and nanoemulsions [70,71]. Unfortunately, it has to be noticed that most technological solu-tions on the nanoscale are only promising at the laboratory stage.…”