The new method for studying neuronal activity: Optogenetics

Ерофеев, А. И.; Matveev, M. V.; Terekhin, S. G.; Захарова, О. А.; Plotnikova, P. V.; Власова, О. Л.

doi:10.1016/j.spjpm.2015.12.001

Cited by 2 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another common method is delivering the materials by adeno‐associated viruses (AAV) and the Cre‐dependent AAV expression system, for which the optimal gene expression time is 3 weeks after injection. The disadvantages of the three delivery methods are similar and present insufficient specificity and a low level of expression for several cell‐specific promoters 9,10 …”

Section: Neurobiologymentioning

confidence: 99%

“…The beam parameters required for studying fast oscillations in thin brain slices using several opsins in vitro are different from those for studying the effects of prolonged in vivo stimulation of certain regions of the animal brain. 10 The two most commonly used opsin genes that have been injected into the mouse brain are ChR2 (derived from C. reinhardtii) and NpHR (derived from N. pharaonis). When ChR2 is expressed within a neuron, it produces a cation channel that is sensitive to blue light; when activated by blue light, the ion channel opens, allowing Na + ions to enter the neuron to cause an excitatory depolarization.…”

Section: Neurobiologymentioning

confidence: 99%

“…11,12 For ChR2, ChR2(H134R), ChR1/2 chimeras, and ChE-TA (derived from C. reinhardtii) opsins family, the excitation wavelength is 470 nm, and the main function of these opsins is cellular membrane depolarization. 10 In addition, they are capable of fast on/off switching, which is why these opsins are best suited for the precise activation of neurons. NpHR inhibition is frequently used in neurons activity studies once it was able to induce a stable photocurrent with an excitation wavelength at 590 nm.…”

Section: Neurobiologymentioning

confidence: 99%

“…21 VChR1 (derived from Volvox carteri) has an excitation wavelength of 535 nm with a cellular membrane depolarization function and a redshift in the activation spectrum (relative to ChR2) allows exercising combined control. 10 Finally, both opto-α1AR and opto-β2AR (obtained synthetically) have an excitation wavelength of 500 nm with a biochemical control function over the cellular membrane. This group of opsins includes light-activated GPCRs (activated via the G-protein).…”

Section: Neurobiologymentioning

confidence: 99%

“…This group of opsins includes light-activated GPCRs (activated via the G-protein). 10 Different illumination parameters are used for controlled optical activation of opsin-expressing cells. The wavelength of the light source is tuned to the maximum activation peak of the opsin.…”

Section: Neurobiologymentioning

confidence: 99%

See 4 more Smart Citations

The state of the art of biomedical applications of optogenetics

Neghab

Soheilifar²,

Grusch

et al. 2021

Lasers Surg Med

View full text Add to dashboard Cite

Background and objective Optogenetics has opened new insights into biomedical research with the ability to manipulate and control cellular activity using light in combination with genetically engineered photosensitive proteins. By stimulating with light, this method provides high spatiotemporal and high specificity resolution, which is in contrast to conventional pharmacological or electrical stimulation. Optogenetics was initially introduced to control neural activities but was gradually extended to other biomedical fields. Study design In this paper, firstly, we summarize the current optogenetic tools stimulated by different light sources, including lasers, light‐emitting diodes, and laser diodes. Second, we outline the variety of biomedical applications of optogenetics not only for neuronal circuits but also for various kinds of cells and tissues from cardiomyocytes to ganglion cells. Furthermore, we highlight the potential of this technique for treating neurological disorders, cardiac arrhythmia, visual impairment, hearing loss, and urinary bladder diseases as well as clarify the mechanisms underlying cancer progression and control of stem cell differentiation. Conclusion We sought to summarize the various types of promising applications of optogenetics to treat a broad spectrum of disorders. It is conceivable to expect that optogenetics profits a growing number of patients suffering from a range of different diseases in the near future.

show abstract

Section: Neurobiologymentioning

confidence: 99%

Section: Neurobiologymentioning

confidence: 99%

Section: Neurobiologymentioning

confidence: 99%

Section: Neurobiologymentioning

confidence: 99%

Section: Neurobiologymentioning

confidence: 99%

See 3 more Smart Citations

The state of the art of biomedical applications of optogenetics

Neghab

Soheilifar²,

Grusch

et al. 2021

Lasers Surg Med

View full text Add to dashboard Cite

show abstract

Optogenetic and chemogenetic modulation of astroglial secretory phenotype

Салмина

Gorina

Ерофеев

et al. 2021

Reviews in the Neurosciences

View full text Add to dashboard Cite

Astrocytes play a major role in brain function and alterations in astrocyte function that contribute to the pathogenesis of many brain disorders. The astrocytes are attractive cellular targets for neuroprotection and brain tissue regeneration. Development of novel approaches to monitor and to control astroglial function is of great importance for further progress in basic neurobiology and in clinical neurology, as well as psychiatry. Recently developed advanced optogenetic and chemogenetic techniques enable precise stimulation of astrocytes in vitro and in vivo, which can be achieved by the expression of light-sensitive channels and receptors, or by expression of receptors exclusively activated by designer drugs. Optogenetic stimulation of astrocytes leads to dramatic changes in intracellular calcium concentrations and causes the release of gliotransmitters. Optogenetic and chemogenetic protocols for astrocyte activation aid in extracting novel information regarding the function of brain’s neurovascular unit. This review summarizes current data obtained by this approach and discusses a potential mechanistic connection between astrocyte stimulation and changes in brain physiology.

show abstract

The new method for studying neuronal activity: Optogenetics

Cited by 2 publications

References 43 publications

The state of the art of biomedical applications of optogenetics

The state of the art of biomedical applications of optogenetics

Optogenetic and chemogenetic modulation of astroglial secretory phenotype

Contact Info

Product

Resources

About